Isolation of an Oocyte Stimulatory Peptide from the Ovarian Follicular Fluid of Water Buffalo (Bubalus bubalis)

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Gupta, P.S.P.;Ravindra, J.P.;Nandi, S.;Raghu, H.M.;Ramesha, K.P.

  • 투고 : 2004.09.26
  • 심사 : 2005.05.04
  • 발행 : 2005.11.01

초록

Ovarian follicular fluid contains both stimulatory and inhibitory agents that influence the growth and maturation of oocyte. In the present study, an attempt was made to isolate and study the biological properties of ovarian follicular fluid peptide(s) in buffaloes. Bubaline ovarian follicular was made steroid- and cell-free. A protein fraction was obtained by saturation (30-35% level) of the follicular fluid with ammonium sulfate. The protein fraction was purified with Sephadex-G 50 gel filtration chromatography and a single peak was obtained in the eluant volume, which was lyophilized. SDS-PAGE of the lyophilized fraction revealed a single band and the molecular weight of the peptide was 26.6 kDa. The peptide stimulated the cumulus cell expansion and in vitro maturation rate of oocytes in buffaloes in a dose dependent manner when it was incorporated at different dose levels (0, 10, 25, 50, 100 and 1,000 ng $ml^{-1}$ of maturation medium). The basic culture medium consisted of TCM 199 with Bovine serum albumin (0.3%). The in vitro maturation rates were comparable to those obtained with a positive control medium (TCM 199+20 ng EGF $ml^{-1}$+steer serum (20%)). Further purification and biological assays may throw more light on the nature and functions of this peptide.

키워드

Follicular Fluid;Peptide;Oocyte;IVM;Buffalo

참고문헌

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피인용 문헌

  1. 1. vol.193, pp.4, 2008, doi:10.5713/ajas.2005.1557
  2. 2. Effect of Fibroblast Growth Factor 2 (FGF2) and Insulin Transferrin Selenium (ITS) on In Vitro Maturation, Fertilization and Embryo Development in Sheep vol.58, pp.4, 2015, doi:10.5713/ajas.2005.1557
  3. 3. Proteome profiling of bovine follicular fluid-specific proteins and their effect on in vitro embryo development vol.79, pp.5, 2017, doi:10.5713/ajas.2005.1557

과제정보

연구 과제 주관 기관 : Indian Council of Agricultural Research