Primary dermal irritation study of oregano oil in rabbits

토끼에서 oregano oil의 일차 피부 자극시험

  • Shin, Jin-Young (College of Veterinary Medicine, Chonnam National University) ;
  • Shin, Dong-Ho (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University) ;
  • Bae, Chun-Sik (College of Veterinary Medicine, Chonnam National University) ;
  • Park, Seung-Chun (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Ki-Hyun (Biomist Co.) ;
  • Bae, Ju-Hyun (Korea Institute of Toxicology, KRICT) ;
  • Kim, Jong-Choon (College of Veterinary Medicine, Chonnam National University)
  • 신진영 (전남대학교 수의과대학) ;
  • 신동호 (전남대학교 수의과대학) ;
  • 김성호 (전남대학교 수의과대학) ;
  • 배춘식 (전남대학교 수의과대학) ;
  • 박승춘 (경북대학교 수의과대학) ;
  • 김기현 ((주)바이오미스트) ;
  • 배주현 (한국화학연구원 부설 안전성평가연구소) ;
  • 김종춘 (전남대학교 수의과대학)
  • Accepted : 2005.02.24
  • Published : 2005.03.25


The present study was carried out to investigate the skin irritation potential of oregano oil in rabbits. A volume of 0.5 ml of test article was applied to intact and abraded skins, respectively, for 24 h in 6 healthy male New Zealand White rabbits. Parameters measured during 72 h observation period were mortality, clinical signs, body weight changes, and local irritation. Treatment-related toxic symptoms, as evidenced by anorexia and decreased locomotor activity, were observed in all rabbits tested. Two rabbits out of the 6 total died on day 2 after the application of test article due to treatment-related toxicity. The mean body weight of test animals decreased progressively during the 72 h observation period. All animals showed very slight edema at 24 h and then recovered at 72 h. These animals also showed very slight to severe erythema and/or slight eschar formation at both 24 h and 72 h. The eschar formation induced by oregano oil was not recovered at the end of additional 11-day recovery period. Based on these results, it was concluded that a single dermal application of the undiluted oregano oil caused an increased incidence of clinical signs and death and a decrease in the body weight and showed a primary irritation index score of 2.79 indicating moderate irritation.


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