Antibiotic resistance pattern of E. coli and Salmonella spp. isolated from chicken feces

닭 분변유래 E. coli 및 Salmonella spp.의 항생제 내성패턴

  • Lee, Young-Ju (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Ae-Ran (National Veterinary Research and Quarantine Service, MAF) ;
  • Jung, Suk-Chan (National Veterinary Research and Quarantine Service, MAF) ;
  • Song, Si-Wook (National Veterinary Research and Quarantine Service, MAF) ;
  • Kim, Jae-Hong (National Veterinary Research and Quarantine Service, MAF)
  • Accepted : 2005.02.13
  • Published : 2005.03.25

Abstract

The purpose of this study was to investigate the antibiotic resistance pattern of E. coli and Salmonella spp. isolated from chicken feces. One hundred and forty-seven E. coli isolates showed resistance to tetracycline (95.2%), erythromycin (89.2%), ampicillin (70.1%), streptomycin (59.2%), cephalothin (56.5%), sulfamethoxazole/trimethoprim (53.7%), ciprofloxacin (57.1%), enrofloxacin (59.2%) and norfloxacin (57.1%). The multiple resistance was seen in 144 isolates (97.9%) and the rate of five, six and seven drugs resistance pattern were 20.4%, 18.4% and 16.3%, respectively. Also, the multiple resistance of E. coli to twelve drugs were seen in 1 isolates (0.7%). Fourteen Salmonella spp. showed resistance to ampicillin (50.0%), streptomycin (57.1%), erythromycin (64.3%) and tetracycline (57.1%) and the rate of two and three drugs resistance pattern were 4 isolates (28.6%), respectively. The prevalence of resistant organisms in Korea probably reflects lack of proper antibiotic policy resulting in prolonged and indiscriminate use of antimicrobial agents.

Acknowledgement

Supported by : 식품의약품안전청

References

  1. 김종만, 진남섭, 김종완, 진영화, 이희수, 권창희, 우승룡, 이해천, 박종명, 김재학, 이재진. 가축의 설사변에서 분리한 대장균과 살모넬라균의 항균물질 감수성과 마우스에서의 치료효과. 대한수의학회지. 1997, 37, 389-404
  2. Chulasiri M, Suthienkul O. Antimicrobial resistance of Escherichia coli isolated from chickens. Vet Microbiol 1989, 21, 189-194 https://doi.org/10.1016/0378-1135(89)90032-1
  3. Lanz R, Kuhnert P, Boerlin P. Antimicrobial resistance and resistance gene determinants in clinical Escherichia coli from different animal species in Switzerland. Vet Microbiol 2003, 91, 73-84 https://doi.org/10.1016/S0378-1135(02)00263-8
  4. Longlois BE, Dawson KA, Leak I, Aaron DK. Antimicrobial resistance of fecal coliforms from pigs in a herd not exposed to antimicrobial for 126 months. Vet Microbiol 1988, 18, 147-153 https://doi.org/10.1016/0378-1135(88)90060-0
  5. OIE, European Scientific Conference. The use of antibiotics in animals ensuring the protection of public health. 1999
  6. van den Bogaard AE, London N, Dreissem C, Stobberingh EE. Antibiotic resistance of faecal Escherichia coli in poultry, poultry farmers and poultry slaughters. J Antimicrob Chemother 2001, 47, 763-771 https://doi.org/10.1093/jac/47.6.763
  7. 하준일, 홍기성, 송시욱, 정석찬, 민영식, 신형철, 이기옥, 임경종, 박종명. 축산 및 수의분야의 항생물질 사용설태 조사. 한국수의공중보건학회지. 2003, 27, 205-217
  8. Chaslus DE, Gerbaud G, Lagorce M, Lafont JP, Courvalin P. Persistance of an antibiotic resistance plasmid in intestinal Escherichia coli of chickens in the absence of selective pressure. Antimicrob Agent and Chemother 1987, 31, 784-788 https://doi.org/10.1128/AAC.31.5.784
  9. National Committee for Clinical Laboratory Standards. Methods for Dilution Antimicrobial Susceptibility Test for Bacteria That Grow Aerobically; Aproved Standard, 5th ed. M7-A5. 2000
  10. Saenz Y, Zarazaga M, Brinas L, Lantero M, Ruiz-Larrea F, Torres C. Antibiotic resistance in Escherichia coli isolates obtained from animals, food and humans in Spain. Int J Antimicrob Agents 2001, 18, 353-358 https://doi.org/10.1016/S0924-8579(01)00422-8
  11. Albertini MT, Benoit C, Berardi L, Berrouane Y, Boisivon A, Cahen P, Cattoen C, Costa Y, Darchis P, Deliere E, Demontrond D, Golliot F, Grise G, Harel A, Koeck JL, Lepennec MP, Malbrunot C, Marcollin M, Maugat S, Nouvellon M, Pangon B. Surveillance of methicillin-resistant Staphylococcus aureus (MRSA) and Enterobacteriaceae producing extended-spectrum beta-lactamase (ESBLE) in Northern France a five-year multicentre incidence study. J Hosp Infect 2002, 52, 107-113 https://doi.org/10.1053/jhin.2002.1286
  12. Ewing WH. Edwards and Ewing's Identification of Enterobacterioaeae, 4th ed. pp. 181-318, Elsevier, Amsterdam, 1996
  13. 국립수의과학검역원. 국립수의과학검역원 고시 제 2002-3호 축산물의 가공기준 및 성분규격 . 2002. 150181
  14. Guerra B, Junker E, Schroeter A, Malorny B, Lehmann S, Helmuth R. Phenotypic and genotypic characterization of antimicrobial resistance in German Escherichia coli isolates from cattle, swine and poultry. J Antimicrob Chemother 2003, 52, 489-492 https://doi.org/10.1093/jac/dkg362
  15. Tanaka MK, Ishihara K, Morioka A, Kojima A, Ohzono T, Ogikubo K, Takahashi K, Tamur YA. National surveillance of antimicrobial resistance in Escherichia coli isolated from food-producing animals in Japan. J Antimicrob Chemother 2003, 51, 447-451 https://doi.org/10.1093/jac/dkg014
  16. 윤은선, 박석기, 오형희, 김태종. 동물원의 야생동물 분변에서 분리한 살모넬라균의 생물형, 혈청형 및 약제감수성. 대한수의학회지. 1994, 34, 267-273
  17. 이경원. 국내항생물질 내성균 모니터링. 국립독성연구원 용역최종보고서. 2002, 23-25
  18. Ozawa Y, Tanimoto K, Nomura T, Yoshinaga M, Arakawa Y, Ike Y. Vancomycin-resistant enterococci in humans and imported chickens in Japan. Appl Environ Microbiol 2002, 68, 6457-6461 https://doi.org/10.1128/AEM.68.12.6457-6461.2002
  19. Chiou CS, Jones AL. Expression and identification of strA-strB gene pair from streptomycin-resistant Erwinia amylovira. Gene 1995, 152, 47-51 https://doi.org/10.1016/0378-1119(94)00721-4
  20. Chung ME, Yeo SG. Distribution of antimicrobial resistance and properties of R-plasmid in E. coli isolated from pigs. Korean J Vet Res 1994, 34, 759-868
  21. Esaki H, Morioka A, Kojima A, Ishihara K, Asai T, Tamura Y, Izumiya H, Terajima J, Watanabe H, Takahashi T. Epidemiological Characterization of Salmonella Typhimurium DT104 Prevalent among Food-Producing Animals in the Japanese Veterinary Antimicrobial Resistance Monitoring Program (1999-2001). Microbiol Immunol 2004, 48, 553-556 https://doi.org/10.1111/j.1348-0421.2004.tb03542.x
  22. Schwarz S, Chaslus-Dancla E. Use of antimicrobials in veterinary medicine and mechanisms of resistance. Vet Res 2001, 32, 201-225 https://doi.org/10.1051/vetres:2001120
  23. Tomita H, Pierson C, Lim SK, Clewell DB, Ike Y. Possible connection between a widely dissemiated conjugative gentamicin resistance (pMG1-like) plasmid and the emergence of vancomycin resistance in Enterococcus faecium. J Clin Microbiol 2002, 40, 3326-3333 https://doi.org/10.1128/JCM.40.9.3326-3333.2002
  24. Mathew AG, Saxton AM, Upchurch WG, Chatin SE. Multiple Antibiotic Resistance Patterns of Escherichia Coli Isolated form Swine farms. Appl Environ Microbiol 1999, 65, 2770-2772
  25. Lee YJ, Kim KS, Kwon YK, Tak RB. Biochemical characteristics and antimicrobials susceptibility of Salmonella gallinarum isolated in Korea. J Vet Sci 2003, 4, 161-166
  26. Price KE, Defuria MD, Pursiano TA. Amikacin, an aminoglicoside with marked activity against antibioticresistant clinical isolates. J Infect Dis 1976, 134, 249-261 https://doi.org/10.1093/infdis/135.Supplement_2.S249
  27. Sorum H, Sunde M. Resistance to antibiotics in the normal flora of animals. Vet Res 2001, 32, 227-241 https://doi.org/10.1051/vetres:2001121
  28. Mastour S, Al-Ghamdi FE, Zaki H, Al Mustafa A, Mohammad H. Antibiotic resistance of Eschsrichia coli isolated from poultry workers, patients and chicken in the eastern province of Saudi Arabia. Tropical Medicine and International Health 1999, 4, 278-283 https://doi.org/10.1046/j.1365-3156.1999.00392.x
  29. Song JH. Emerging infectious disease due to microbial adaptation : Emergence and spread of antimicrobial resistance. Korean J Infect Dis 1999, 31, 79-87 https://doi.org/10.1080/00365549950161934
  30. Orden JA, Ruiz-Santa-Quiteria JA, Cid D, Diez R, Martinez S, de la Fuente R. Quinolone resistance in potentially pathogenic and non-pathogenic Escherichia coli strains isolated from healthy ruminants. J Antimicrob Chemother 2001, 48, 421-424 https://doi.org/10.1093/jac/48.3.421
  31. FDA. Center for drug evaluation and research. Antimicrobial Resistance. 2003
  32. Liebana E, Garcia-Migura L, Clouting C, Clifton-Hadley FA, Lindsay E, Threlfall EJ, McDowell SW, Davies RH. Multiple genetic typing of Salmonella enterica serotype typhimurium isolates of different phage types (DT104, U302, DT204b, and DT49) from animals and humans in England, Wales, and Northern Ireland. J Clin Microbiol 2002, 40, 4450-4456 https://doi.org/10.1128/JCM.40.12.4450-4456.2002
  33. Teshager T, Herrero IA, Porrero MC, Garde J, Moreno MA, Dominguea L. Surveillance of antimicrobial resistance in Escherichia coli strains isolated from pigs at Spanish slaughterhouses. Int J Antimicrob Agents 2000, 15, 137-142 https://doi.org/10.1016/S0924-8579(00)00153-9