Antigenetic effects of the eluted proteins from house dust mite (Dermatophagoides pteronyssinus) in dogs infested with sarcoptic mite (Sarcoptes scabiei var. canis)

집먼지진드기에서 분리한 용출단백질의 개옴진드기 감염증에 대한 항원효과

  • Kim, Tae-Hun (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Jae-Won (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Jee, Cha-Ho (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • 김태훈 (충북대학교 수의과대학 및 동물의학연구소) ;
  • 김재원 (충북대학교 수의과대학 및 동물의학연구소) ;
  • 지차호 (충북대학교 수의과대학 및 동물의학연구소)
  • Accepted : 2005.01.19
  • Published : 2005.03.25

Abstract

Canine sarcoptic mite (Sarcoptes scabiei var. canis) is ectoparasite which burrow usually in the stratum corneum of the skin of dogs. Antigens from the burrowing mites induce humoral and cellmediated immune responses in the hosts. The effect of antigenecity induced by somatic antigens of house dust mite (Dermatophagoides pteronyssinus) isolated by continuous elution has been evaluated in canine sarcoptic mites infestation. Continuous elution was carried out in 7.5% SDS-PAGE to isolate proteins of common antigens from somatic antigens of house dust mite. These eluted proteins from somatic antigens of house dust mite were confirmed by Western blotting in 7.5% SDS-PAGE, and eluted proteins (65, 60 kDa) were isolated. To evaluate the antigenetic effect of eluted proteins, eight dogs were divided as 4 groups such as non-vaccinated and non-challenged control (Group I), challenged control (Group II), vaccinated (Group III), and vaccinatedandchallenged (Group IV) groups. Group II and IV were artificially infested canine sarcoptic mites. Group III and IV were immunized with eluted proteins (65, 60 kDa). At the 6th week of the vaccination, the antibody titers of Group of IV were statistically significant higher than those of Group II (p<0.05). And antibody titers of Group III were also statistically significant higher than those of Group I (p<0.05). From these result, it is possible to replace somatic antigens of canine sarcoptic mites with eluted proteins from somatic antigens of house dust mites in order to diagnose and prevent the canine sarcoptic mite infestations.

Acknowledgement

Supported by : 충북대학교

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