Quantitation of L-carnitine in plasma by electrospray ionization tandem mass spectrometry

ESI/MS/MS를 이용한 혈장 중 카르니틴 정량분석

  • Kang, Seung Woo ;
  • Kim, Ho Hyun ;
  • Lee, Kyung Ryul ;
  • Yoon, Hye-Ran
  • 강승우 ;
  • 김호현 ;
  • 이경률 ;
  • 윤혜란
  • Received : 2005.01.12
  • Accepted : 2005.03.08
  • Published : 2005.04.25

Abstract

In this study, a novel analytical method has been developed for the rapid determination of L-carnitine in human plasma using electrospray ionization tandem mass spectrometry. Free carnitine (FC) was analyzed after extraction with 80% methanol and total carnitine (TC) was analyzed after hydrolysis and extraction. Acyl carnitine (AC) was subtracted FC from TC. Analytical methods used multiple reaction monitoring (MRM) scan modes. A correlation coefficient of linear regression ($r^2$) was 0.9995, recovery was 97%, reproducibility was less than 10%, and limit of detection (LOD) was $0.0016{\mu}mol/L$. This method reduced sample preparation time and showed high resolution and good reproducibility compared to that with liquid chromatographic methods. Normal control showed AC was lower than FC. Clinical management of patients with inborn error of metabolism showed FC was lower than AC. Thus, carnitine fraction level was very important to monitoring patients with metabolic disorder.

Keywords

IR;NMR;MMPs;Zymography;SK-Hept-1;MTT

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