Effects of Lactobacillus reuteri-based Direct-fed Microbial Supplementation for Growing-Finishing Pigs

  • Shon, K.S. (Dept. of Animal Resource and Sciences, Dankook University) ;
  • Hong, J.W. (Dept. of Animal Resource and Sciences, Dankook University) ;
  • Kwon, O.S. (Dept. of Animal Resource and Sciences, Dankook University) ;
  • Min, B.J. (Dept. of Animal Resource and Sciences, Dankook University) ;
  • Lee, W.B. (Dept. of Animal Resource and Sciences, Dankook University) ;
  • Kim, I.H. (Dept. of Animal Resource and Sciences, Dankook University) ;
  • Park, Y.H. (Probionic, Inc.) ;
  • Lee, I.S. (Probionic, Inc.)
  • Received : 2004.03.17
  • Accepted : 2004.11.03
  • Published : 2005.03.01


Two experiments were conducted to evaluate the effects of direct-fed microbial supplementation on the growth performance and nutrient digestibility in pigs. In experiment 1, forty eight pigs were used in a 42-d growth assay. There were four pigs per pen and three pens per treatment. Dietary treatments included 1) NC (without antibiotic basal diet), 2) PC (NC diet+0.1% antibiotic, 100 g/kg chlortetracycline), 3) DFM-1 (NC diet+0.2% Lactobacillus reuteri and Lactobacillus salivarius complex) and 4) DFM-2 (NC diet+0.2% Lactobacillus reuteri, Lactobacillus salivarius, Lactobacillus plantarum and Yeast complex). For the overall period, no treatment had significant effects in growth performance. In the nutrition digestibility, the pigs fed DFM diets were improved in DM and N digestibility compared with the pigs fed NC and PC diets but it was not significantly different. In experiment 2, sixty four crossbred pigs were used in a 98-d growth assay. There were four pigs per pen and four pens per treatment. Dietary treatments included 1) HND (high nutrient diet), 2) LND (low nutrient diet), 3) HND+DFM (HND diet+0.2% Lactobacillus reuteri and Lactobacillus plantarum complex) and 4) LND+DFM (LND diet+0.2% Lactobacillus reuteri and Lactobacillus plantarum complex). For overall period of growing phase, the pigs fed LND diets had improved gain/feed (p=0.01) and for overall period in the finishing phase, the pigs fed LND with DFM diets had higher ADG, ADFI and gain/feed than the others but there were no significant differences (p>0.13). In total period of growing-finishing phase, the pigs fed LND diet had higher gain/feed than the pigs fed HND diets (p<0.05). In growing phase, there were not significant differences among the treatments means for DM and N digestibility. However, the pigs fed diets with DFM had improved N digestibility (p<0.02) compared to the pigs fed diets without DFM in finishing phase. In conclusion, DFM slightly improved the growth performance in growing-finishing pigs.


Direct Fed Microbial;Growth Performance;Pigs


  1. Hong, J. W., I. H. Kim, O. S. Kwon, J. H. Kim, B. J. Min and W. B. Lee. 2002. Effects of dietary probiotics supplementation on growth performance and fecal gas emission in nursing and finishing pigs. J. Anim. Sci. Technol. Kor. 44:305-314.
  2. Lyons, T. P. and J. D. Chapman. 1990. Probiotics. In: Nontraditional feed use in sources swine production. (Ed. P. A. Thacker and R. N. Kirkwood). Butterworth Publishers, MA. pp. 315-326.
  3. Perdigon, G., S. Alvarez, M. Rachid, G. Aguero and N. Gobbato. 1995. Immune system stimulation by probiotics. J. Dairy Sci. 78:1597-1606.
  4. Jin, L. Z., Y. W. Ho, N. Abdullah and S. Jalaudin. 1997. Probiotics in poultry: modes of action. World’s Poult. Sci. J. 53:351-368.
  5. Kyriakis, S. C., I. Georgoulakis, A. Spais, C. Alexopoulos, C. C. Miliotis and S. K. Kritas. 2003. Evaluation of Toyocerin, a Probiotic Containing Bacillus toyoi Spores, on Health Status and Productivity of Weaned, Growing and Finishing Pigs. Asian-Aust. J. Anim. Sci. 16:430-434.
  6. Huang, Canghai, Shiyan Qiao, Defa Li, Xiangshu Piao and Jiping Ren. 2004. Effects of Lactobacilli on the performance, diarrhea incidence, VFA conentration and gastrointestinal microbial flora of weaning pigs. Aaian-Aust. J. Anim. Sci. 17:401-409.
  7. Harper, A. F., E. T. Kornegay, K. L. Bryant and H. R. Thoman. 1983. Efficacy of virginiamycin and a commercially-available Lactobacillus probiotic in swine diets. Anim. Feed Sci. Technol. 8:69-76.
  8. Xuan, Z. N., J. D. Kim, K. N. Heo, H. J. Jung, J. H. Lee, Y. K. Han, Y. Y. Kim and In K. Han. 2001. Study on the development of a probiotics complex for weaned pigs. Asian-Aust. J. Anim. Sci. 14:1425-1428.
  9. Shornikova, A. V., I. A. Casas, E. Isolauri, H. Mykkanen and T. Vesikari. 1997. Lactobacillus reuteri as a therapeutic agent in acute diarrhea in young children. J. Pediatr. Gastroenterol. Nutr. 24:399-404.
  10. Chiang, S. H. and W. M. Hsieh. 1995. Effect of direct-fed microorganisms on broiler growth performance and litter ammonia level. Asian-Aust. J. Anim. Sci. 8:159-162.
  11. Kornegay, E. T. and C. R. Risley. 1996. Nutrient digestibilities of a corn-soybean meal diets as influenced by Bacillus products fed to finishing swine. J. Anim. Sci. 74:799-805.
  12. Kyriakis, S. C., V. K. Tsiloyiannis, J. Vlemmas, K. Sarris, A. C. Tsinas, C. Alexopoulos and L. Jansegers. 1999. The effect of probiotic LSP 122 on the control of post-weaning diarrhoea syndrome of piglets. Res. Vet. Sci. 67:223-228.
  13. Mukai, T., T. Asasaka, E. Sato, K. Mori, M. Matsumoto and H. Ohori. 2002. Inhibition of binding of Helicobactor pylori to the glycolipid receptor by probiotic Lactobacillus reuteri. FEMS Immun. Med. Microbiol. 32:105-110.
  14. Nousiainen, J. and J. Setala. 1993. Lactic acid bacteria as animal probiotics. In: Lactic acid bacteria. (Ed. S. Salminen and A. von Wright). Marcel Dekker, Inc., New York. USA.
  15. Yang, S. J., J. S. Hyon, C. B. Yang, S. M. Ko and H. H. Choi. 1998. Studies on the effects of feed additives fed to pigs: Effects of feeding probiotics on the growth performance and carcass quality in pigs. Kor. J. Anim. Sci. 40:21-30.
  16. Chang, Y. H., J. K. Kim, H. H. Kim, W. Y. Kim, Y. B. Kim and Y. H. Park. 2000. Probiotics effects of Lactobacillus reuteri BSA-131 on piglets. Kor. J. Appl. Microbial. Biotechnol. 28:8-13.
  17. Fialho, E. T., M. Vassalo, J. A. F. Lima and A. G. Bertechine. 1998. Probiotics utilization for piglets from 10 to 30 kg (performance and metabolism assay). The 8th World Conference on Animal Production Contributed Papers 1:622-623.
  18. Pollmann, D. S., D. M. Danielson and E. R. Peo Jr. 1980. Effects of microbial feed additives on performance of starter and growing-finishing pigs. J. Anim. Sci. 51:577.
  19. Fuller, R. 1989. Probiotics in man and animals - A Review. J. Appl. Bacterol. 66:365-378.
  20. NRC. 1998. Nutrient Requirements of Swine. National Reserch Council, Academy Press.

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