- Volume 18 Issue 7
DOI QR Code
Muscle Proteome Analysis for the Effect of Panax Ginseng Extracts in Chicken: Identification of Proteins Using Peptide Mass Fingerprinting
- Jung, K.C. (1Division of Animal Science and Resources, College of Agriculture and Life Sciences Chungnam National University) ;
- Yu, S.L. (Division of Animal Science and Resources, College of Agriculture and Life Sciences Chungnam National University) ;
- Lee, Y.J. (Division of Animal Science and Resources, College of Agriculture and Life Sciences Chungnam National University) ;
- Choi, K.D. (The Graduate School of Bio and Information Technology, Hankyong National University) ;
- Choi, J.S. (Proteome Analysis Team, Korea Basic Science Institute) ;
- Kim, Y.H. (Proteome Analysis Team, Korea Basic Science Institute) ;
- Jang, B.G. (National Livestock Research Institute, R.D.A.) ;
- Kim, S.H. (Department of Biological Science, Kyunghee University) ;
- Hahm, D.H. (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
- Lee, J.H. (Division of Animal Science and Resources, College of Agriculture and Life Sciences Chungnam National University)
- Received : 2004.09.22
- Accepted : 2005.02.18
- Published : 2005.07.01
The present study was aimed to investigate proteome affected by Panax ginseng extracts in chicken muscles. The whole muscle proteins from chicken fed boiled extracts of 0% (control), 1%, 3%, and 5% Panax ginseng in water were separated by two-dimensional electrophoresis (2-DE) gels using immobilized non-linear gradient (pH 3-10) strips. More than 300 protein spots were detected on silver staining gels. Among them, four protein spots were distinctively up-regulated by Panax ginseng treatments and further investigated by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The obtained MS data were searched against SwissProt database using the Mascot search engine. The up-regulated proteins were finally identified as
Chicken;Muscle;MALDI-TOF MS;2DE;Panax Ginseng
Supported by : Rural Development Administration
- Concha, C., S. Hu and O. Holmberg. 1996. The proliferative responses of cow stripping milk and blood lymphocytes to pokeweed mitogen and ginseng in vitro. Vet. Res. Commun. 27(2):107-115.
- Hwang, I. H. 2004. Application of gel-based proteome analysis techniques to studying post-mortem proteolysis in meat. Asian-Aust. J. Anim. Sci. 17(9):1296-1302. https://doi.org/10.5713/ajas.2004.1296
- Moon, K. S. 1999. Ingredients of medicinal herbs and their use. Ilweolseogak.
- Pandey, A. and M. Mann. 2000. Proteomics to study genes and genomes. Nature 405:837-846. https://doi.org/10.1038/35015709
- Wang, W., K. Ouyang, J. Ouyang, H. Li, S. Lin and H. Sun. 2004. Polymorphism of insulin-like growth factor I gene in six chicken breeds and its relationship with growth traits. Asian-Aust. J. Anim. Sci. 17(3):301-304.
- Lees-Miller, J. P. and D. M. Helfman. 1991. The molecular basis for tropomyosin isoform diversity. Bioessays. 13(9):429-437.
- Talamo, F., C. D'Ambrosio, S. Arena, P. Del Vecchio, L. Ledda, G. Zehender, L. Ferrara and A. Scaloni. 2003. Proteins from bovine tissues and biological fluids: defining a reference electrophoresis map for liver, kidney, muscle, plasma and red blood cells. Proteomics 3:440-460.
- Heukeshoven, J. and R. Dernick. 1985. Simplified method for silverstaining of proteins in polyacrylamide gels and the mechanism of silver staining. Electrophoresis 6:103-112.
- Casal, J. I., T. J. Ahern, R. C. Davenport, G. A. Petsko and A. M. Klibanov. 1987. Subunit interface of triosephosphate isomerase: site-directed mutagenesis and characterization of the altered enzyme. Biochemistry. 26(5):1258-1264.
- Ationu, A., A. Humphries, B. Wild, T. Carr, A. Will, R. Arya and D. M. Layton. 1999. Towards enzyme-replacement treatment in triosephosphate isomerase deficiency. Lancet. 353:1155-1156.
- Hu, S., C. Concha, A. Johannisson, G. Meglia and K. P. Waller. 2001. Effect of subcutaneous injection of ginseng on cows with subclinical staphylococcus aureus mastitis. J. Vet. Med. B. 48:519-528.
- Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Chem. 72:248-254.
- Hu, S., C. Concha, R. Cooray and O. Holmberg. 1995. Ginsengenhanced oxidative and phagocytic activities of polymorphonuclear leukocytes from bovine peripheral blood and stripping milk. Vet. Res. 26:155-161.
- Park, M. J., J. H. Song, H. P. Lee and Y. C. Kim. 1990. Effects of the protein fraction of Panax ginseng on primary cultured chicken skeletal muscle cells. Kor. J. Pharmacogn. 21(3):210-216.