Inhibition of human breast carcinoma by BLC (Sargassum fulvellum) and BLC/HEN Egg in vitro and in vivo

  • Jo, Eun-Hye (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Cho, Sung-Dae (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Ahn, Nam-Shik (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Jung, Ji-Won (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Yang, Se-Ran (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Park, Joon-Suk (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Hwang, Jae-Woong (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Lee, Sung-Hoon (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Park, Jung-Ran (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Sun-Jung (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Park, Hyun-Kyung (Sam-Hwa Farm) ;
  • Lee, Yong-Soon (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Kang, Kyung-Sun (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
  • Accepted : 2005.02.21
  • Published : 2005.03.25

Abstract

Much of the interest on the chemopreventive properties of herbs and plants has been raised, whereas little is regarding to anti-tumor effect of farming and aquatic products. In the present study, the anti-tumor effect of hot-water extract of a seaweed, BLC (Sargassum fulvellum) and BLC/HEN egg was investigated using MCF-7 cells in vitro and in vivo systems. We found that the BLC extract and BLC/HEN egg inhibited cell proliferation in a dose-dependent manner, which might be mediated through up-regulation of p53. Furthermore, this test compound can directly induce apoptosis in MCF-7 cells, which might be mediated through up-regulation of a pro-apoptotic Bax protein and down-regulation of a anti-apoptotic Bcl-2 protein, not by immune system. Nude mice bearing established breast tumors (with exogenous estradiol) were treated with BLC extract and BLC/HEN egg. Treatment BLC extract and BLC/HEN egg caused a 42% and 71% inhibition of tumor growth, respectively. Both agents caused a significant inhibition of volume and weight growth of estrogen independent human breast tumors established from MCF-7 cells. Our results suggested that BLC extract and BLC/HEN egg have the efficacious effect of human breast cancer not only in vitro but also in vivo.

Acknowledgement

Supported by : Seoul National University

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