- Volume 18 Issue 3
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Effect of Transinoculation of Goat Rumen Liquor on Degradation and Metabolism of Mimosine in Sheep Fed with Leucaena leucocephala Leaves
- Vaithiyanathan, S. (Central Sheep and Wool Research Institute) ;
- Sheikh, Q. (Central Sheep and Wool Research Institute) ;
- Kumar, Ravindra (Central Sheep and Wool Research Institute)
- Received : 2004.04.09
- Accepted : 2004.08.25
- Published : 2005.03.01
The effect of transinoculation of goat rumen liquor into sheep rumen on mimosine toxicity was studied. One adult Kutchi male goat having higher mimosine degradation capacity than sheep was gradually adapted to Leucaena leucocephala (Leucaena) leaves by feeding increasing level of eucaena leaves supplementation for 1 month. Six Bharat Merino rams (12-18 months of age) were divided into two equal groups with (group I) or without (group II) infusion of 200 ml of goat rumen liquor per animal. The mimosine degradation in groups I and II were 3.04 and 2.31; 3.90 and 3.73 mg per day per 10 ml rumen liquor respectively after 1 and 2 weeks of leucaena feeding leaves. Total rumen bacterial population in RGCA medium and in a selective medium containing iron showed an increasing trend in both groups, while the bacterial population growing in the presence of cellulose showed a decreasing trend. Animal performance data did not show any adverse effect. Results revealed that transinoculation of rumen liquor from leucaena leaves adapted goat to sheep rumen did not help to improve mimosine degradation in the sheep. The sheep transinoculated with goat rumen liquor displayed no in vivo improvements in nutrient utilization vis-a-vis mimosine metabolism.
- Allison, M. J. 1991. Simplified cultural test for ruminal bacteria that degrade toxic dihydroxy pyridine derivatives of mimosine. Leucaena Res. Rep. 12:105-108.
- Allison, M. J., A. C. Hammond and R. J. Jones. 1990. Detection of ruminal bacteria that degrade toxic dihydroxypyridine compounds produced from mimosine. Appl. Environ. Microbiol. 56:590-594.
- AOAC. 1984. Official methods of analysis. 14th edn. Association of Official Analytical Chemists, Washington, DC.
- Aregheore, E. M. 2002. Voluntary intake and digestibility of fresh, wilted and dry lecaena (Leucaena leucocephala) at four levels to a basal diet of Guinea grass (Panicum maximum). Asian-Aust. J. Anim. Sci. 15:1139-1146. https://doi.org/10.5713/ajas.2002.1139
- Bakshi, M. P. S. and M. Wadhwa. 2004. Evaluation of forest tree leaves of semi-hilly arid region as livestock feed. Asian-Aust. J. Anim. Sci. 17:777-783.
- D'Mello, J. P. F. 1992. Chemical constraints to the use of tropical legumes in animal nutrition. Anim. Feed Sci. Technol. 38:237-261.
- Dominguez-Bello, M. G. and C. S. Stewart. 1990. Degradation of mimosine, 2,3-dihydroxypyridine and 3-hydroxy-4 (1H)-pyridone by bacteria from the rumen of sheep in Venezuela. FEMS MIcrobiol. Ecol. 73:263-290.
- Gomez, K. A. and A. A. Gomez. 1976. Statistical procedure for agricultural research, 2nd edition AWiley International publication, Singapore pp. 84-129.
- Gupta, H. K. and P. P. Atreja. 1998. Influence of gradual adaptation of cattle to Leucaena leucocephala leaf meal on biodegradation of mimosine and 3-hydroxy-4(1H)-pyridone (3,4-DHP) in rumen, their levels in blood, fate and influence of absorbed DHP on thyroid hormone and liver enzymes. Anim. Feed Sci. Tech. 74:29-43.
- Hammond, A. C. 1995. Leucaena toxicosis and its control in ruminants. J. Anim. Sci. 73:1487-1492. https://doi.org/10.2527/1995.7351487x
- Hart, N. K., A. Hofman, J. A. Lamberto and C. M. Richards. 1977. Mimosine, mimosinamine and 3,4-dihydroxypyridine. Heterocycles. 7:265-272.
- Hegarty, M. P., P. G. Schinckel and R. D. Court. 1964. Reaction of sheep to the consumption of Leucaena glauca benth. and to its toxic principle mimosine. Aust. J. Agric. Res. 15:153-167.
- Holdeman, L. V., E. P. Cato and W. E. C. Moore. 1977. Anaerobe laboratory manual 4th edn. Anaerobe Laboratory, Virginia Polytechnic Institute and State University, Blacksburg, USA.
- Hungate, R. E. 1970. A roll tube method for cultivation of strict anaerobes. In: Methods in microbiology. Ed. R. Norris and D. W. Ribbons. Vol. 3B. Academic Press Inc., New York, pp. 117-132.
- Jones, R. J. 1994. Management of anti-nutritive factors- with special reference to leucaena. In: Forage tree legumes in Tropical Agriculture (Ed. R. C. Gutteridge and H. M. Shelton). CAB International, UK. pp. 216-231.
- Jones, R .J. 1979. The value of Leucaena leucocephala as a feed for ruminants in the tropics. World Anim. Rev. 31:13-23.
- Jones, R. J. and M. P. Hegarty. 1984. The effect of different proportions of Leucaena leucocephala in the diet of cattle on growth, feed intake, thyroid function and urinary excretion of 3-hydroxy-4(1H)-pyridone. Aust. J. Agric. Res. 35:317-325.
- Jones, R. J. and J. B. Lowry. 1984. Australian goats detoxify the goitrogen 3-hydroxy-4 (1H)-pyridone after rumen infusion from Indonesian goat. Experientia. 40:1435-1436.
- Jones, R. J. and R. G. Megarrity. 1986. Successful transfer of DHP degrading bacteria from Hawaiian goats to Australian ruminants to overcome the toxicity of Leucaena. Aust. Vet. J. 63:259-262.
- Kudo, H., K. J. Cheng, W. Majak, J. W. Hall and J. W. Costerton. 1984. Degradation of mimosine in rumen fluid from cattle and sheep in Canada. Can. J. Anim. Sci. 64:937-942.
- Kumar, R. and J. P. F.D'Mello. 1995. Anti-nutritional factors in forage legumes. In: Tropical legumes in Animal Nutrition (Ed. J. P. F. D'Mello and C. Devendra). CAB Internation, UK. pp. 95-134.
- Mc Dougall, E. L. 1948. Studies on ruminant saliva.I. The composition of sheep’s saliva. Biochem. J. 43:99.
- Oliva, M. L. V., J. C. Souza-pinto, F. C. Batista, I. M. S. Arango, V. F. Silveira, E. A. Auerswald, R. Mentele, C. Eckerskorn, M. U. Sampaio and C. A. M. Sampaio. 2000. Leucaena leucocephala serine protease inhibitor: primary structure and action of blood coagulation, kinin release and rat paw edema. Biochim. Biophys. Acta. 1477:64-74.
- Oppenheim, E. W., I. M. Nasrallah, M. G. Mastri and P. J. Stover. 2000. Mimosine is a cell specific antagonist of folate metabolism. J. Biol. Chem. 275:19268-19274.
- Pathak, P. S., R. Deb Roy and S. K. Gupta. 1983. Fodder trees as additional resources of fuel, gum, dyes and other useful products. In: Top feed resources, Their production, utilization and the constraints. (Ed. M. Singh). Central Sheep and Wool Research Institute, Avikanagar, p. 60-70.
- Pratchett, D., R. J. Jones and F. X. Syrch. 1991. Use of DHP degrading rumen bacteria to overcome toxicity in cattle grazing irrigated leucaena pasture. Trop. Grass. 25:268-274.
- Quirk, M. F., J. J. Bushell, R. J. Jones, R. G. Megarrity and K. L. Butler. 1988. Liveweight gains on leucaena and native grass pastures after dosing cattle with rumen bacteria capable of degrading DHP, a ruminal metabolite from leucaena. J. Agric. Sci. 111:165-170.
- Ranjhan, S. K. 1998. Nutrient requirements of livestock and poultry. Indian Council of Agricultural Research, New Delhi pp. 9-14.
- Semenye, P. P. 1990. Toxicity response of goats fed on Leucaena leucocephala forage only. Small Rum. Res. 3:617-620. https://doi.org/10.1016/0921-4488(90)90056-C
- Singh, S., S. S. Kundu, A. S. Negi, S. K. Gupta, N. P. Singh and V. C. Pachauri. 2002. Leucaena seeds as protein supplements in the rations of growing sheep. Asian-Aust. J. Anim. Sci. 15:1433-1438.
- Stewart, C. S. and S. H. Duncan. 1985. The effect of avoparcin on cellulolytic bacteria of the ovine rumen. J. Gen. Microbiol. 131:427-435.
- Tangendjaja, B. and R. B. H.Willis. 1980. Analysis of mimosine and 3-hydroxyu-4 (1H)-pyridone by high performance liquid chromatography. J. Chromat. 202:317-318.
- Tilley, J. M. A. and R. A. Terry. 1963. A two stage technique for in vitro digestion of forage crops. J. Br. Grass. Soc. 18:104-111.
- Tudsri, S. and C. Kaewkunya. 2002. Effect of leucaena row spacing and cutting intensity on the growth of leucaena and three associated grasses in Thailand. Asian-Aust. J. Anim. Sci. 15:986-991.
- Vaithiyanathan, S., R. Kumar, Q. Sheikh and J. P. Mishra. 1998. Mimosine degradation by mixed rumen microbes of sheep and goat. Indian J. Small Rum. 4:94-98.
- Van Soest, P. J., J. B. Roberston and B. A. Lewis. 1991. Methods for dietry fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597.
- Wanapat, M., N. Nontaso, C. Yuanklang, S. Wora-anu, A. Nagarmsang, C. Wachirapakorn and P. Rowlinson. 20003. Comparative study between swam buffalo and native cattle in feed digestibility and potential transfer of buffalo rumen digesta into cattle. Asian-Aust. J. Anim. Sci. 16:504-510.
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