Replacing Soybean Meal (SBM) by Canola Meal (CM) : The Effects of Multi-enzyme and Phytase Supplementation on the Performance of Growing and Laying Quails

  • Saricicek, B. Zehra (Department of Animal Science, Faculty of Agricultural, University of Ondokuzmayıs) ;
  • Kilic, U. (Department of Animal Science, Faculty of Agricultural, University of Ondokuzmayıs) ;
  • Garipoglu, A.V. (Department of Animal Science, Faculty of Agricultural, University of Ondokuzmayıs)
  • 투고 : 2004.11.11
  • 심사 : 2005.03.18
  • 발행 : 2005.10.01


In this research, the possibilities of using canola meal (CM) in place of soybean meal (SBM), and also the effects of multi-enzyme and phytase supplementation on the performance of quails were investigated. For this purpose, soybean meal (44% CP), canola meal (37% CP), phytase (produced from Peniophora luci) and multi-enzyme ($\beta$-glucanases, pectinases, cellulases and hemicellulases) were used. CM was used supplying 0, 25 and 50% of CP from SBM and each of the phytase and multi-enzyme blends were added to the each level. This study was conducted with 675 day old quails (Coturnix coturnix Japonica) in 9 groups with 3 replicates including 25 birds (mixed sex) per replicate. Nine isocalaric and isonitrogenous diets were prepared. The effects of enzymes and CM levels were studied with a 3${\times}$3${\times}$3 factorial arrangement for three CM levels (0, 25 and 50%), three treatments (without enzyme, phytase enzyme and multi-enzyme) and three replicates. While the 25% CM level did not affect the liveweight gain 50% CM level decreased the liveweight gain (p<0.05). Multi-enzyme addition to the 50% CM group increased the liveweight gain compared to the other groups (p<0.05). CM levels and enzyme supplementation had no effect on feed consumption, feed conversion ratio, dressing percentage, viability, tibia ash content, Ca and P contents of tibia ash, viscera weight, gizzard weight and length of growth period. While heart weight and liver weight were not affected by CM levels, but they were affected by enzyme supplementation. CM levels and enzyme supplementation did not affect final liveweight, feed consumption, feed conversion ratio, egg yield, egg weight, shell weight and shell index during laying period. The increase in the CM level lightened the colour of the yolk (p<0.05).


  1. Bourdon, D. and A. Aumaitre. 1990. Low-glucosinolate rapeseeds and rapeseed meals: effect of technological treatments on chemical composition, digestible energy content and feeding value for growing pigs. Anim. Feed Sci. Technol. 30:175-191.
  2. Broz, J., P. Oldale, H. Perrin-Voltz, G. Rychen, J. Schulze and C. Simoes Nunes. 1994. Effect of supplemental phytase on performance and phosphorus utilization in broiler chickens fed a low phosphorus diet without addition of inorganic phosphates. Br. Poult. Sci. 35:273-280.
  3. Campbell, L. D., D. Liang, B. A. Slominski and W. Guenter. 2001. Nutritive value of enzyme pre-treated canola meal, Proceedings International Symposium on rapeseed Science. April, 19-23. Wuhan, The People’s Republic of China. pp. 334-342.
  4. Guenter, W., L. W. J. Nernberg and B. A. Slominski. 1997. Improved phosphorus availability in poultry fed wheat/canola meal-based diets supplemented with phytase enzyme. Proceeding of International Symposium on Rapeseed Science. April 19-23, Wuhan, The People’s Republic of China, 315-327.
  5. Hygheabert, G. and D. Groote. 1995. The effect of specific enzymes on the Men-value and nutrient utilization of target feedstuff in broiler and layer diets. WPSA Proceedings, 10th European Symposium on Poultry Nutrition, October 15-19, Antalya, Turkey.
  6. Pointillart, A. 1991. Enhancement of phosphorus utilization in growing pigs fed phytate-rich diets by using rye bran. J. Anim. Sci. 69:1109-1115.
  7. Newkirk, R. W. and H. L. Classen. 2002. The Effects of toasting canola meal on body weight, feed conversion efficiency and mortality in broiler chickens. Poult. Sci. 81:815-825.
  8. O’Dell, B. L. and A. R. deBorland. 1976. Complexation of phytate with proteins and cations in corn germ and oilseed meal. J. Agric. Food Chemist. 24(4):804-808.
  9. Choct, M. 2002. Non-starch polysaccharides:effects on nutritive value. In: Poultry Feedstuffs:Supply, Composition and Nutritive Value, pp. 221-235. (Ed. J. M. McNab and K. N. Boorman), CABI Publishing.
  10. Schoner, F. J., P. P. Hoppe and G. Schwarz. 1991. Comparative effects of microbial phytase and inorganic phosphorus on performance and on retention of phosphorus, calcium and crude ash in broilers. J. Anim. Physiol. Anim. Nutr. 66:248-255.
  11. Ravindran, V., W. L. Bryden and E. T. Kornegay. 1995. Phytases: Occurrence, bioavailability, and implication in poultry nutrition. Poult. Avian Biol. Rev. 6:125-143.
  12. Campbell, G. L. and M. R. Bedford. 1992. Enzyme applications for monogastric feeds:a review, Can. J. Anim. Sci. 72:449-466.
  13. Ikegami, S., F. Tsuchihashi, H. Harada, N. Tsuchihashi, E. Nishide and S. Innami. 1990. Effect of viscous indigestible polysaccharides on pancreatic-bilary secretion and digestive organs in rats. J. Nutr. 120:335-360.
  14. Leeson, S., J. O. Atteh and J. D. Summers. 1987. The replacement value of canola meal for soybean meal in poultry diets. Canadian J. Anim. Sci. 67:151-158.
  15. Schoner, F. J., G. Schwarz, P. P. Hoppe and H. Wiesche. 1994. Effect of microbial phytase on Ca-availability in broilers. Third Conferance of pig and poultry nutrition. Halle, Germany, November 29-December 1:235-244.
  16. Duncan, D. B. 1955. Multiple range and multiple F tests. Biometrics. 11:1-42.
  17. Kocher, A., M. Choct, M. D. Porter and J. Broz. 2000. The effects of enzyme addition to broiler diets containing high levels of canola or sunflower meal. Poult. Sci. 79:1767-1774.
  18. Bedford, M. R. and A. J. Morgan. 1995. The Use of Enzymes in Canola-based Diets. In: 2ndEuropean Symposium on feed enzymes. Noordwijkerhout. Netherlands. 125-131.
  19. Bustany, Z. A. and K. Elwinger. 1988. Whole grains, unprocessed rapeseed and $\beta$-glucanase in diets for laying hens. Swedish J. Agric. Res. 18:31-40.
  20. Kocher, A., M. Choct, L. Morrisrose and J. Broz. 2001. Effects of enzyme supplementation on the replacement value of canola meal for soybean meal in broiler diets. Aust. J. Agric. 52:447-452.
  21. Simbaya, J., B. A. Slominski, W. Guenter, A. Morgan and L. D. Campbell. 1996. The effects of protease and carbohydrase supplementation on the nutritive value of canola meal for poultry: In vitro and in vivo studies, Anim. Feed Sci. Tecnl. 61:219-234.
  22. AOAC. 1990. Official Methods of Analysis. 15th Ed. Association of Official Analytical Chemists, Arlington, VA.
  23. Bell, J. M. 1993. Factors affecting the nutritional value of canola meal: A review. Canadian J. Anim. Sci. 73:679-697.
  24. Borcea, F., A. Csuma and D. Grossu. 1996. The partial replacement of soybean meal with canola meal in broiler diets. Archiva Zootechnica 4:69-76.
  25. Hamilton, R. M. G. and F. G. Proudfoot. 1993. Effects of dietary barley level on the performance of leghorn hens. Can. J. Anim. Sci. 73:625-634.
  26. Chesson, A. 1993. Feed enzymes. Anim. Feed Sci. Tecnol. 45:65-79.
  27. Gomez, S., C. Moj. ca and S. R. Fernandez. 1993. Effect of Peniophora Lycii phytase on nutrient utilization in laying hens. Mexico Agricultural Research Institute Roche Vitam. Mexico S.A. de C.V.
  28. Sebastian, S., S. P. Touchburn, E. R. Chavez and P. C. Lague. 1996. Efficacy of supplemental microbial phytase at different dietary calcium levels on growth performance and mineral utilization of broiler chickens. Poult. Sci. 75:1516-1523.
  29. Slominski, B. A. and L. D. Campbell. 1990. Non-Starch Polysaccharides of Canola Meal:Quantification, Digestibility İn Poultry and Potential Benefit of Dietary Enzyme Supplementation. J. Sci. Food Agric. 53:175-184.
  30. Benabdeljlil, K. and M. I. Arbaoui. 1994. Effect of enzyme supplementation of barley-based diets on hen performance and egg quality. Anim. Feed Sci. Technol. 48:325-334.
  31. Shim, Y. H., B. J. Chae and J. H. Leel. 2004. Effects of phytase and enzyme complex supplementation to diets with different nutrient levels on growth performance and ileal nutrient digestibility of weaned pigs. Asian-Aust. J. Anim. Sci. 17:523-532.
  32. Bedford, M. R. and H. Classen. 1992. Reduction of intestinal viscosity through manipulation of dietary rye and pentosanase concentration is affected through changes in the carbohydrate changes in the carbohydrate composition of the intestinal aqueous phase and results in improved growth rate feed conversion, J. Nutr. 122:260-269.
  33. Shires, A. J., J. R. Thompson, B. V. Turner, P. M. Kennedy and Y. K. Goh. 1987. Rate of passage of corn-canola meal and cornsoybean meal diets through the gastrointestinal tract of broiler and white leghorn chickens, Poult. Sci. 66:289-298.

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