A Study of matrix metalloproteinase-9 inhibitor in root bark of ulmus davidiana planchon

유근피내의 Matrix Metalloproteinase-9 활성 억제제에 관한 연구

  • 공광훈 (중앙대학교 자연과학대학 화학과) ;
  • 한기정 (중앙대학교 의과대학 생화학교실) ;
  • 이광수 (중앙대학교 자연과학대학 화학과) ;
  • 조성희 (중앙대학교 자연과학대학 화학과)
  • Received : 2004.12.27
  • Accepted : 2005.01.18
  • Published : 2005.04.25

Abstract

Several solvents were used to fractionate an extract obtained from the chapped root bark of Ulmus davidiana Planchon. The each fractionary part was condensed under reduced pressure and then examined to investigate the inhibitory effect on MMPs by modified gelatin zymography, where EA fraction showed the inhibition effect on the activity of MMPs. A compound showing inhibition effect on the MMPs was isolated and purified from EA fraction. Under IR, $^1H$- and $^{13}C$- NMR analyses it is very close to a catethin. This substance showed 48% inhibition effect on measurement of MMP-9 activity at 5 mM and 43% at 10 mM. To verify the effect of this substance on cells, human hepatoma, SK-Hep-1 cells as a cancer model, and Chang liver cells as a normal model were selected. MTT assay was performed to examine the cell viability by treatment of $1{\mu}L/mL$ of the purified substance on cells. The purified substance showed negligible toxicity on human liver cell line.

Keywords

Ulmus davidiana planchon;IR;NMR:MMPs;zymography;SK-hept-1;MTT

Acknowledgement

Supported by : 중앙대학교

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