A case of sex determination by amplification of SRY and Amelogenin gene in horse

SRY와 Amelogenin gene의 증폭에 의한 말의 성(sex) 결정 예

  • Cho, Gil-jae (Laboratory of Equine Genetics, Korea Racing Association) ;
  • Lee, Sun-young (Laboratory of Equine Genetics, Korea Racing Association) ;
  • Yang, Young-jin (Laboratory of Equine Genetics, Korea Racing Association)
  • 조길재 (한국마사회 유전자검사실) ;
  • 이선영 (한국마사회 유전자검사실) ;
  • 양영진 (한국마사회 유전자검사실)
  • Accepted : 2004.12.22
  • Published : 2005.03.25

Abstract

The objective of present study was to ascertain sex determination for individual identification, parentage control, and sex chromosome anomalies in horse. PCR amplification products of the equine sex determining region of the Y chromosome gene (SRY) and amelogenin gene (AMEL) were detected by using agarose gel electrophoresis. A normal sire and foal II showed 1 SRY band (430 bp) and 3 AMEL (AMELX, AMELY, and AMELX/Y) band, 175 bp, 160 bp, 190 bp, respectively, and a normal dam and foal I showed a single AMELX band (175 bp). These results enables a quick diagnosis for sex determination prior to cytogenetic analysis.

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