Control of Diatrype stigma occurred on the bed-log of Shiitake by resistant Shiitake strains

표고골목 해균인 주홍꼬리버섯을 방제하기 위한 저항성 표고균주 선발

  • Lee, Bong-Hun (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute) ;
  • Bak, Won-Chull (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute) ;
  • Ka, Kang-Hyeon (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute) ;
  • Yoon, Kab-Hee (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute) ;
  • Park, Hyun (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute) ;
  • Cha, Byeong-Jin (Department of Plant Medicine, Chungbuk National University)
  • 이봉훈 (국립산림과학원 화학미생물과) ;
  • 박원철 (국립산림과학원 화학미생물과) ;
  • 가강현 (국립산림과학원 화학미생물과) ;
  • 윤갑희 (국립산림과학원 화학미생물과) ;
  • 박현 (국립산림과학원 화학미생물과) ;
  • 차병진 (충북대학교 식물의학과)
  • Published : 2005.09.30

Abstract

Attempts were made to control Diatrype stigma occurred on the bed-log of shiitake by resistant shiitake strains. In selection test of resistant shiitake strains, 67 out of 77 strains tested were proved to be resistant to D. stigma. Among them, 13 strains including KFRI 5 were effective to inhibit the access of D. stigma, and 7 strains including KFRI 180 remarkably invaded the territory of D. stigma. Among 31 shiitake strains made by hybridization of resistant strains for D. stigma, 8 strains including KFRI 537 inhibited the access of D. stigma, and 4 strains including KFRI 545 invaded the territory of D. stigma. The effects of temperatures and inoculation orders to the resistance were confirmed in PDA plates and test tubes filled with sawdust of Quercus acutissima. Four kinds of temperature treatments as follows were tested: (1) continuous incubation at $14^{\circ}C$, (2) continuous incubation at $25^{\circ}C$, (3) changing of incubation temperature from $14^{\circ}C$ to $25^{\circ}C$ as soon as mycelia of both shiitake and D. stigma meet together, (4) changing of incubation temperature from $25^{\circ}C$ to $14^{\circ}C$ as soon as mycelia of both shiitake and D. stigma meet together. Three kinds of inoculation procedure were tested: (1) inoculation of shiitake 3 days ahead of D. stigma inoculation, (2) inoculation of D. stigma 3 days ahead of shiitake inoculation, (3) simultaneous inoculation of both fungi. In PDA plate test, the strain KFRI 137 showed outstanding ability to inhibit mycelial growth of D. stigma and the strain KFRI 180 invaded into the territory of D. stigma in most of treatments. Hybrid strains, KFRI 545, 546, and 547 were more resistant than their parent strains, KFRI 488 and 405. In test tube examinations, all the strains of shiitake showed high resistance at the treatment of change in temperature from $14^{\circ}C$ to $25^{\circ}C$ when mycelia of both shiitake and D. stigma meet together. On the other hand, resistance of all the strains growing at $25^{\circ}C$ decreased when the temperature was changed into $14^{\circ}C$ after mycelia of both fungi. In these cases, the resistance reached to 7~20% of the highest resistance. The strain KFRI 259 invaded the territory of D. stigma, contrary to PDA plate test. Among the strains, KFRI 393 strain was the most resistant under the continuous incubation at $25^{\circ}C$.

Keywords

Diatrype stigma;Hybrid strains;Quercus acutissima;Shiitake