Biological Activities and Artificial Cultivation of Cordyceps pruinosa Petch

붉은자루동충하초의 자실체 증식 특성

  • Hong, In-Pyo (Division of Sericulture and Entomology, National Institute of Agricultural Science and Technology, R.D.A) ;
  • Nam, Sung-Hee (Division of Sericulture and Entomology, National Institute of Agricultural Science and Technology, R.D.A) ;
  • Jung, I-Yeon (Division of Sericulture and Entomology, National Institute of Agricultural Science and Technology, R.D.A) ;
  • Sung, Gyoo-Byung (Division of Sericulture and Entomology, National Institute of Agricultural Science and Technology, R.D.A) ;
  • Kim, Hyun-Bok (Division of Sericulture and Entomology, National Institute of Agricultural Science and Technology, R.D.A) ;
  • Jung, In-Mo (Division of Sericulture and Entomology, National Institute of Agricultural Science and Technology, R.D.A) ;
  • Cho, Soo-Muk (Division of Agriproduct processing, National Institute of Agricultural Science and Technology, R.D.A) ;
  • Lee, Min-Woong (Dept. of Applied Biology, Dongguk University)
  • 홍인표 (농업과학기술원 잠사양봉소재과) ;
  • 남성희 (농업과학기술원 잠사양봉소재과) ;
  • 정이연 (농업과학기술원 잠사양봉소재과) ;
  • 성규병 (농업과학기술원 잠사양봉소재과) ;
  • 김현복 (농업과학기술원 잠사양봉소재과) ;
  • 정인모 (농업과학기술원 잠사양봉소재과) ;
  • 조수묵 (농업과학기술원 농산물가공이용과) ;
  • 이민웅 (동국대학교 생물학과)
  • Published : 2005.12.31

Abstract

Fruiting bodies of Cordyceps have been regarded as popular folk and effective medicines to treat human diseases such as asthma, bronchial and lung inflammation, and kidney disease. Cordyceps pruinosa (Clavicipitaceae; Hypocreales; Ascomycotina) has received special attention for medicinal purpose due to its various physiological activites. The nucleoside derivative N6-(2-hydroxyethyl) adenosine (HEA) isolated from it showed a $Ca^{2+}$ antagonistic effect and negative inotropic response. The artificial production of fruiting body of C. pruinosa has not been tried successfully yet by using living silkworm substrate. To develop techniques for the production of C. pruinosa stromata on a large scale, the infection of Bombyx mori with C. pruinosa and the growth characteristics of stroma of C. pruinosa were investigated. Also, studied about biological activities of fruiting body formed on silkworm. Infection rate of the silkworm pupae with C. pruinosa was the highest in injection inoculation. The formation of the fruiting body of C. pruinosa was quite good in the room controlled at $21{\sim}25^{\circ}C$, over 91% of relative humidity and over 1500 lx. Glucose concentration was high in the fruiting bodies of the silkworm pupae infected with C. pruinosa on a dry weight basis. The most abundant amino acid in the fruiting bodies was arginine and phenylalanine. The fruiting bodies of silkworm pupae infected with C. pruinosa was rich in oleic acid. The high amount of citric acid was found in the fruiting bodies of silkworm pupae infected with C. pruinosa.

Keywords

Amino acid;Cordyceps pruinosa;Injection inoculation;Silkworm Pupae