Effects of Using Cold Water on Mixing Sawdust Media for Flammulina velutipes

고온기 팽이버섯 병재배 배지제조시 저온수 이용 효과

  • Cheong, Jong-Chun (Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Jhune, Chang-Sung (Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Kim, Seung-Hwan (Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Won, Hang-Yeon (Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Kwon, Jae-Geon (Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration)
  • 정종천 (농업과학기술원 응용미생물과) ;
  • 전창성 (농업과학기술원 응용미생물과) ;
  • 김승환 (농업과학기술원 응용미생물과) ;
  • 원향연 (농업과학기술원 응용미생물과) ;
  • 권재건 (농업과학기술원 응용미생물과)
  • Published : 2005.12.31

Abstract

This study was conducted to verify the cause of suppression symptom in mycelial growth during summer and to be able to establish a countermeasure. Cultivation of Flammulina velutipes was experimented with varying elapsed time of 0, 3, 6, 9 hours after mixing the sawdust media and two kinds of water temperature (normal water, $24^{\circ}C$ and cold water, $6^{\circ}C$) for mixing sawdust media. There were trends of increased media temperature from $24^{\circ}C$ to $31^{\circ}C$ and decreased pH from 6.5 to 5.2~5.6 with varying elapsed time from mixing the media to sterilization. Bacterial density also increased with bacterial density in Medium $24^{\circ}C$ being 1.9~4.1 times higher than that in Medium 6. Growth of F. velutipes was delayed with dual culture of bacteria isolated from sawdust media. Total nitrogen content of sawdust media was lowered by elapsed time from mixing the media to sterilization. The use of normal water($24^{\circ}C$) delayed mushroom growth by 1~2 days compared with that of cold water($6^{\circ}C$). Furthermore, mycelial growth of F. velutipes in the bottle cultivation ceased 9 hours after mixing the media. Primordia formation of F. velutipes was delayed by 1~3 days by elapsed time after mixing sawdust media, while fruit-body yield decreased by 7~12% 6 hours after mixing the media. Fruit-body yield was more stabilized with the use of cold water($6^{\circ}C$) than with that of normal water($24^{\circ}C$). Results showed that it is effective to use cold water as low as $6^{\circ}C$ in mixing media for F. velutipes cultivation, especially during summer when environmental temperature is high, high pressure sterilization after bottling work can prevent bacterial propagation in the media and can stabilize media ingredient.

Keywords

Winter mushroom;Flammulina velutipes;Bottle cultivation;Cooling water