Synaptic Organization of Vibrissa Afferent Terminals in the Trigeminal Interpolar Nucleus

삼차신경중간핵에서 저역치기계자극수용기 유래 들신경섬유 종말의 연접양상

  • Ahn, Hyoung-Joon (Dept. of Oral Medicine, College of Dentistry, Yonsei University) ;
  • Paik, Sang-Kyoo (Dept. of Oral Anatomy, School of Dentistry, Kyungpook National University) ;
  • Bae, Yong-Chul (Dept. of Oral Anatomy, School of Dentistry, Kyungpook National University) ;
  • Choi, Jong-Hoon (Dept. of Oral Medicine, College of Dentistry, Yonsei University) ;
  • Kim, Chong-Youl (Dept. of Oral Medicine, College of Dentistry, Yonsei University)
  • 안형준 (연세대학교 치과대학 구강내과학교실) ;
  • 백상규 (경북대학교 치과대학 구강해부학교실) ;
  • 배용철 (경북대학교 치과대학 구강해부학교실) ;
  • 최종훈 (연세대학교 치과대학 구강내과학교실) ;
  • 김종열 (연세대학교 치과대학 구강내과학교실)
  • Published : 2005.03.30

Abstract

In order to evaluate the mechanism of transmission as well as processing of sensory information originating from low-threshold mechanoreceptor in oral and maxillofacial region at primary synaptic region of trigeminal nervous system, vibrissa afferent fibers of adult cat were labeled with intra-axonal HRP injection. Serial sections containing labeled boutons were obtained from the piece of trigeminal interpolar nucleus. Under electron microscope, total 30 labeled boutons were observed, and ultrastructural characteristics, frequency of occurence, synaptic organizations of vibrissa afferent terminals were analysed. The results were as follows: 1. Labeled boutons contained clear, spherical synaptic vesicles with diameter of 45$\sim$55nm. They formed asymmetrical synapse with dendrites showing definite postsynaptic density, larger synaptic cleft, multiple synaptic structures at various regions. With unlabeled axon terminals(p-ending) containing polymorphic synaptic vesicles, they formed symmetrical synapse showing indefinite postsynaptic density and narrower synaptic area. 2. Each labeled bouton formed 1 to 15 synapses, the average of 4.77$\pm$3.37 contacts per labeled bouton, with adjacent neuronal profiles. Relatively complex synaptic organization, which formed synapses with more than 5 neuronal profiles, was observed in a large number(46.7%, n=14) of labeled boutons. 3. Axo-somatic synapse was not observed. The number of axo-dendritic synapse was 1.83$\pm$1.37 per labeled bouton. Majority(85.0%) of axo-dendritic synapses were formed with dendritic shafts, nonprimary dendrites(n=47, 1.57$\pm$1.38/1 bouton), however, synapses formed with primary dendrites(n=6, 0.20$\pm$0.41/1 bouton) or dendritic spines(n=2, 0.07$\pm$0.25/1 bouton) were rare. 4. 76.7%(n=23) of labeled boutons formed axo-axonic synapse (2.93$\pm$2.36/1 bouton) with p-endings containing pleomorphic vesicles. Synaptic triad, in which p-endings formed synapses with labeled boutons and dendrites adjacent to the labeled boutons simultaneoulsy, were also observed in 60.0%(n=18) of labeled boutons. From the above results, vibrissa afferent terminals of adult cat showed distinctive synaptic organization in the trigeminal interpolar nucleus, thus, suggests their correlation with the function of the trigeminal interpolaris nucleus, which participates in processing of complex sensory information such as two-point discrimination and motivational-affective action. Further studies on physiologic functions such as quantitative analysis on ultrastructures of afferent terminals and nerve transmitters participating in presynaptic inhibition are required.

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