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인삼박으로 배양된 버섯 균사체 추출물이 암세포 증식에 미치는 영향

Effect of Mycelia Extracts of Mushroom-Cultured Ginseng By-product on Proliferation in Cancer Cell Lines

  • 박은미 ((재)경북테크노파크 대구한의대학교 한방생명자원특화센터 효능검증원) ;
  • 김수정 ((재)경북테크노파크 대구한의대학교 한방생명자원특화센터 효능검증원) ;
  • 예은주 ((재)경북테크노파크 대구한의대학교 한방생명자원특화센터 효능검증원) ;
  • 배만종 (대구한의대학교 한방바이오식품과학과) ;
  • 조경철 (풍기우경인삼)
  • Park, Eun-Mi (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technopark) ;
  • Kim, Soo-Jung (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technopark) ;
  • Ye, Eun-Ju (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technopark) ;
  • Bae, Man-Jong (Dept. of Oriental Medicine Biofood Science, Daegu Haany University) ;
  • Jo, Kyeong-Cheol (Korea Bio Ginseng)
  • 발행 : 2005.03.01

초록

인삼박의 가용성 추출물을 용출시킨 후 남은 잔여 묵인 인삼박에 상황버섯, 영지버섯, 운지버섯, 표고버섯 균사체를 접종ㆍ배양하여 얻어진 인삼박 균사체 추출액의 전자공여능 및 간암세포(Hep3B)와 고형암(S-180)의 증식에 미치는 영향을 조사하였다. 인삼박 균사체의 전자공여능은 500-10,000ppm 범위에서 농도가 진할수록 높게 나타났으며 운지가 4종류의 균사체 추출물 중 가장 높았다. 간암세포의 형태변화 및 증식 억 제 에 미 치 는 영 향은 에 탄을 추출물이 물 추출물보다 효과적이었으며, 60% 에탄을 추출물 5,000 ppm에서 상황, 영지,운지에 비해 표고가 99.1%로 가장 강한 세포증식억 제 효과를 나타내었다. S-180 고형 암 억제효과는 대조군에 비해 운지가 65%로 가장 컸으며 영지 61%, 표고 56%, 상황 59%의 고형암 억제효과를 나타내었다. 따라서 인삼박을 이용하여 배양된 버섯 균사체가 간암세포와 고형암의 증식 억제에 효과적인 것으로 관찰되므로써 항암물질의 자원으로서 그 활용성이 기대된다고 하겠다.

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피인용 문헌

  1. Effects of fruiting body extracts of Lentinus edodes cultivated using corn cob on biological activities vol.30, pp.4, 2013, https://doi.org/10.1007/s11814-012-0209-7
  2. Anti-Proliferative Activities of Solid-State Fermented Medicinal Herbs Using Phellinus baumii against Human Colorectal HCT116 Cell vol.20, pp.8, 2010, https://doi.org/10.5352/JLS.2010.20.8.1268
  3. Immunomodulating Effect of Edible Mushrooms in Mice vol.21, pp.4, 2011, https://doi.org/10.5352/JLS.2011.21.4.515
  4. Antioxidant Activity and in vitro for Anticancer Effects of Manufactured Fermented Mulberry Leaf Tea vol.39, pp.6, 2010, https://doi.org/10.3746/jkfn.2010.39.6.796
  5. In Vitro Antioxidant and Anticancer Potential of n-Hexane Extract from Ginseng Marc vol.57, pp.3, 2014, https://doi.org/10.3839/jabc.2014.039