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Stimulation of Macrophage Differentiation by Bu-Zhong-Yi-Qi- Tang Extract

보중익기탕 추출물의 대식세포 분화 유도 효과

  • Kang, Hye-In (Dept. of Food and Nutrition, College of Natural Science, Sunchon National University) ;
  • Shin, Sung-Hae (Dept. of Biology, College of Natural Science, Sunchon National University) ;
  • Cho, Young-Sook (Dept. of Food and Nutrition, College of Natural Science, Sunchon National University) ;
  • Cho, Sung-Ki (Dept. of Food Irradiation, Korea Atomic Energy Research Institute) ;
  • Byun, Mung-Woo (Dept. of Food Irradiation, Korea Atomic Energy Research Institute) ;
  • Yee, Sung-Tae (Dept. of Biology, College of Natural Science, Sunchon National University)
  • 강혜인 (순천대학교 식품영양학과) ;
  • 신성해 (순천대학교 생물학과) ;
  • 조영숙 (순천대학교 식품영양학과) ;
  • 조성기 (원자력연구소 식품ㆍ생명공학팀) ;
  • 변명우 (원자력연구소 식품ㆍ생명공학팀) ;
  • 이성태 (순천대학교 생물학과)
  • Published : 2005.03.01

Abstract

We have investigated the effects of Bu-Zhong-Yi-Qi- ang on the differentiation of murine bone marrow cells in methylcellulose culture. GM-CSF and IL-3 supported primarily the formation of granulocyte/mac-rophage colony formation. However, the addition of Bu-Zhong-Yi-Qi-Tang extract yielded a significant increase in the numbers of colonies and differentiated cells in the presence of GM-CSF and IL-3. We have analyzed CD11b (Mac-1) expression of differentiated cells from bone marrow by staining with monoclonal anti-CD11b antibody. The majority of colony-forming cells were in CD11b/sup +/ population. Also Bu-Zhong-Yi-Qi-Tang extract promoted the production of IL-6 and nitric oxide by macrophages. These results demonstrate that extract of Bu-Zhong-Yi-Qi-Tang, a prescription of traditional oriental medicine, is effective in supporting macrophage potential of the primary colonies.

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