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Antimutagenic and Cytotoxic Effects of Ethanol Extracts from Five Kinds of Seaweeds

다섯 가지 해조류 에탄올 추출물의 항돌연변이 활성 및 암세포 성장억제 효과

  • Kim, Sung-Ae (Dept. of Food and Nutrition, Hannam University) ;
  • Kim, Jin (Dept. of Food and Nutrition, Hannam University) ;
  • Woo, Mee-Kyung (Metamanna Co.) ;
  • Kwak, Chung-Shil (Aging and Physical Culture Research Institute, Seoul National University) ;
  • Lee, Mee-Sook (Dept. of Food and Nutrition, Hannam University)
  • 김성애 (한남대학교 식품영양학과) ;
  • 김진 (한남대학교 식품영양학과) ;
  • 우미경 ((주)메타만나) ;
  • 곽충실 (서울대학교 체력과학노화연구소) ;
  • 이미숙 (한남대학교 식품영양학과)
  • Published : 2005.04.01

Abstract

The protective effects of ethanol extracts from 5 seaweeds on the mutagenic and cytotoxic damage were evaluated. They were separately extracted using ethanol from dried samples at room temperature, and freeze-dried. The inhibition effects on the mutagenicity in Salmonella assay by Ames test and cancer cell inhibitory effect in HeLa cell, MCF-7 cell and SNU -638 cell by MTT assay were assayed. Seaweed fusiforme, sea tangle and green laver showed strong inhibitory effect against 2-nitrofluorene, sodium azide- or 2-anthramine-induced mutagenicities in Salmonella Typhimurium TA 98 and TA 100 at the level of 2.5 mg ethanol extract per plate. Cancer cell inhibitory effect was shown with all of the seaweed extracts. Green laver, sea mustard, sea tangle and seaweed fusiforme showed strong cytotoxicity against HeLa and MCF-7 cells, with inhibiting by $92\~93\%$ and $89\~92\%$, respectively. These data show that 5 seaweeds tested in this study might be potent functional foods for cancer prevention, and consumption of these seaweeds in adequate amount is recommended.

References

  1. Watson RR, Leonard TK. 1986. Selenium and vitamin A, E and C: nutrients with cancer prevention properties. J Am Diet Assoc 86: 505-510
  2. Mohn GR. 1981. Bacterial systems for carcinogenicity testing. Mutat Res 87: 191-210 https://doi.org/10.1016/0165-1110(81)90032-4
  3. Kada T, Morita K, Inoue T. 1978. Antimutagenic action of vegetable factors on the mutagenic principle of tryptophan pyrolysate. Mutat Res 53: 351-353 https://doi.org/10.1016/0165-1161(78)90008-0
  4. Song GS, Ahn BY, Maeong IK, Kwon YJ, Han SB, Choi DS. 1997. Antimutagenicity screening of water extracts from Chinese herbs with SOS Chromotest with several direct mutagens. Food Sci Biotechnol 6: 214-218
  5. Song GS, Ahn BY, Lee GS, Maeng IK, Choi DS. 1997. Effect of hot water extracts from medicinal plants on the mutagenicity of indirect mutagens. Korean J Food Sci Technol 29: 1288-1294
  6. Kuroda Y, Inoue T. 1988. Antimutagenesis by factors affecting DNA repair in bacteria. Mutat Res 202: 387-391 https://doi.org/10.1016/0027-5107(88)90200-X
  7. Chiharu N, Tadashi N, Toshimara Y. 1992. Effect of pH on the in vitro absorption of mutagens to dietary fibers. Biosci Biotech Biochem 56: 1100-1105 https://doi.org/10.1271/bbb.56.1100
  8. Yasuji O, Kiyoka HO. 1994. Identification of antimutagenic activities in the extract of an edible brown algae. Hijikia fusiforme (Hijiki) by ume gene expression system in Salmonella typhimuriurn (TA 1535/pSK 1002). J Sci Food Agric 66: 103-109 https://doi.org/10.1002/jsfa.2740660115
  9. Okai Y, Higashi-Okai K, Nakamura S. 1993. Identification of heterogenous antimutagenic activities in the extract of edible brown seaweeds, Laminaria japonica (Makonbu) and Undaria pinnatifida (Wakame) by the ume gene expression system in Salmonella typhimurium (TA 1535/pSK 1002). Mutat Res 303: 63-70 https://doi.org/10.1016/0165-7992(93)90096-E
  10. Ryu BH, Kim DS, Cho KJ, Sin DB. 1989. Antitumor activity of seaweeds toward sarcoma-180. Korean J Food Sci Technol 21: 595-600
  11. Fujihara M, Nagumo T. 1993. An influence of the structure of alginate on the chemotatic activity of macrophages and antitumor activity. Carbohydrate Research 243: 211-216 https://doi.org/10.1016/0008-6215(93)84094-M
  12. Ebihara K, Kiriyama S. 1990. Physiochemical property and physiological function of dietary fiber. Nippon Shokuhin Kogyo Gakkaishi 37: 916-925 https://doi.org/10.3136/nskkk1962.37.11_916
  13. Kim HS, Kim GJ. 1998. Effects of the feeding Hijikia fusiforme (Harvey) Okamura on lipid composition of serum in dietary hyperlipidemic rats. J Korean Soc Food Sci Nutr 27: 718-723
  14. Hurch FC, Meade JB, Treanor RE, Whinna HC. 1989. Antithrombotic activity of fucoidin with heparin cofactor II, antithrombin III and thrombin. J Biol Chem 6: 361-375
  15. Kim Kl, Seo HD, Lee HS, Jo HY, Yang HC. 1998. Studies on the blood anticoagulant polysaccharide isolated from hot water extracts on Hijikia fusiforme. J Korean Soc Food Sci Nutr 27: 1204-1210
  16. Ito K, Tsuchiya Y. 1972. The effect of algal polysaccharides on the depressing of plasma cholesterol levels in rat. In Proc. of 7th Int. Seaweed Symp. Nishizawa K, ed. Univ. Tokyo Press, Japan. p 558-561
  17. Kim DS, Park YH. 1985. Uronic acid composition, block structure and some related properties of alginic acid. J Korean Fish Soc 18: 29-36
  18. Colliec S, Fischer AM, Tapon-Bretaudiere J, Boisson C, Durand P, Jozefonvicz J. 1991. Anticoagulant properties of a fucoidan fraction. Thromb Res 64: 143-154 https://doi.org/10.1016/0049-3848(91)90114-C
  19. Yamamoto L, Takahashi M, Tamura E, Maruyama H, Mori H. 1984. Antitumor activity of edible marine algae: Effect of crude fucoidan fraction prepared from edible brown seaweeds L-1210 leukemia. Hydrobiologia 116: 145-150 https://doi.org/10.1007/BF00027653
  20. Yamamoto L, Nagumo T, Takahashi M, Fujihara M, Suzuki Y, Lizima N. 1981. Antitumor effect of seaweeds: III. Antitumor effect of an extract from Sargassum. Jap J Exp Med 51: 187-189
  21. Cho KJ, Lee YS, Ryu BH. 1990. Antitumor effect and immunology activity of seaweeds toward sarcoma-180. Bull Korean Fish Soc 23: 345-352
  22. Kim IS, Kim SB, Park YH. 1994. Desmutagenic of seaweed and vegetable extracts against mutagenicity of Maillard reacting products. Bull Korean Fish Soc 27: 139-141
  23. Lee NH, Oh KI. 2002. Screening of radical scavenging effects from marine algae. Cheju J Life Science 3: 95-101
  24. Lee BH, Choi BW. 1996. Extraction of water soluble antioxidants from seaweeds. J Korean Ind & Eng Chemistry 7: 1069-1077
  25. Han HS, Bae SJ, Kim MH. 2004. Effects of Porphyra tenera extracts on formation of collagen cross-link in ovariectomized rats. J Korean Soc Food Sci Nutr 33: 324-330 https://doi.org/10.3746/jkfn.2004.33.2.324
  26. Maron DM, Ames BN. 1983. Revised methods for the Salmonella mutagenicity test. Mutat Res 113: 173-215 https://doi.org/10.1016/0165-1161(83)90010-9
  27. Matsushima T, Sugimura T, Nagao M, Yahagi T, Shirai A, Sawamura M. 1980. Factors modulating mutagenicity in microbial test. In Shotr-term test, systems for detecting carcinogens. Norphth KH, Gamer RC, eds. Springer, Berling. p 273-275
  28. Charmichael J, Degraff WG, Gazdar AF, Minna JD, Michell JB. 1987. Evaluation of a tetrazolium based semiautomated colorimetric assay, assessment of chemosensitivity testing. Cancer Res 47: 936-941
  29. Park YB, Ahn JK, Yoo SJ, Park DC, Kim IS, Park YH, Kim SB. 1998. Elucidation of anti-tumor initiator and promoter derived from seaweed-4: desmutagenic principles of Ecklonia stolonifera extracts against carcinogenic heterocyclic amines. J Korean Soc Food Sci Nutr 27: 537-542
  30. Ryu BH, Chi BS, Kim DS, Ha MS. 1986. Desmutagenic effect of extracts obtained from seaweeds. Bull Kor Fish 19: 502-508
  31. Takahi N, Hiroaki K, Haruki Y, Terukazu N. 1991. An anticoagulant fucoidan from the brown seaweed Ecklonea kurome. Phytochemistry 30: 535-539 https://doi.org/10.1016/0031-9422(91)83722-W
  32. Riou D, Colliec-Jouault S, Pinczon du Sel D, Bosch S, Siavoshian S, Le Bert V, Tomasoni C, Sinquin C, Durand P, Roussakis C. 1996. Antitumor and antiproliferative effects of a fucan extracted from Ascophyllum nodosum against a non-small-cell bronchopulmonary carcinoma line. Anticancer Res 16: 1213-1218
  33. Ellouali M, Boisson-Vidal C, Durand P, Jozefonvicz J. 1993. Antitumor activity of low molecular weight fucans extracted from brown seaweed Ascophyllum nodosum. Anticancer Res 13: 2011-2019
  34. Krinskey NI. 1993. Micronutrients and their influence on mutagenicity and malignant transformation. Ann New York Acad Sci 686: 229-234 https://doi.org/10.1111/j.1749-6632.1993.tb39180.x
  35. Krinskey NI. 1994. Carotenoids and cancer, basic research studies, natural antioxidants in human health and disease. Ann New York Acad Sci 239: 1 -6 https://doi.org/10.1111/j.1749-6632.1974.tb25310.x
  36. Funahashi H, Imai T, Mase T, Sekiya M, Yokoi K, Hayashi H, Shibata A, Hayashi T, Nishikawa M, Suda N, Hibi Y, Mizuno Y, Tsukamura K, Hayakawa A, Tanuma S. 2001. Seaweed prevents breast cancer? Jpn J Cancer Res 92: 483-487 https://doi.org/10.1111/j.1349-7006.2001.tb01119.x

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