Inhibition against Helicobacter pylori and Biological Activities by Rue (Ruta graveolens L.) Extracts

Rue(Ruta graveolens L.) 추출물의 Helicobacter pylori에 대한 항균활성과 생리활성효과

  • Cho, Young-Je (Dept. of Food Engineering, Sangju National University) ;
  • Chun, Sung-Sook (Dept. of Food Science & Technology, Yeungnam University) ;
  • Kim, Jeung-Hoan (Dept. of Food Engineering, Sangju National University) ;
  • Yoon, So-Jung (Dept. of Food Engineering, Sangju National University)
  • 조영제 (상주대학교 식품공학과) ;
  • 천성숙 (영남대학교 식품가공학과) ;
  • 김정환 (상주대학교 식품공학과) ;
  • 윤소정 (상주대학교 식품공학과)
  • Published : 2005.04.01


Water and ethanol extracts from Rue were prepared, and their growth inhibiting activity against Helicobacter pylori and other biological activities were examined. Total phenolic compounds in the water and ethanol extracts were present at the concentration of 16.39 mg/g, and 17.07 mg/g, respectively. At the concentration of $200\;{\mu}g/mL$ of phenolic compounds concentration, water extract produced 12 mm inhibition zone while ethanol extract produced 13 mm. The ABTS [2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)] radical decolorization and antioxidant protection factor (PF) were determined for extracts from Rue. Water extracts showed $96\%$ inhibition rate on ABTS, but ethanol extracts showed higher PF (1.2) than water extracts (0.8). Water extracts had higher electron donation ability on DPPH than ethanol extracts. But ethanol extracts had higher ACE (angiotensin converting enzyme) and xanthine oxidase inhibitory activities than water extracts. Rosemarinic acid and quercetin were the most abundant phenolic compounds as analyzed by HPLC.


  1. Aruoma OI. 1998. Free radical. Oxidative stress and antioxidants in human health and disease. J Am Oil Chem Soc 75: 199-212
  2. Kyrtopoulos SA. 1989. N-nitroso compound formation in human gastric juice. Cancer Surveys 8: 423-442
  3. Rhee KH, Cho MJ, Kim JB, Choi SK, Kim YC. 1988. Bacteriological characteristics of Carnpylobacter pylori. J Kor Soc Microbiol 23: 17-26
  4. Baik SC, King JB, Cho M, Kim YC, Park CK, Ryou HH, Choi HJ, Rhee KH. 1990. Prevalence of H. pylori infection among nornal Korean adults. J Kor Soc Microbiol 25: 455-462
  5. Ho CT. 1992. Phenolic compounds in food. In Phenolic compounds in food and their effects on health II. Huang MT, Ho CT, Lee CY, eds. Maple Press, New York. p 2-7
  6. Ahn YS, Shin DH, Baek NI. 2000. Isolation and identification of active antimicrobial substance against Listeria monocytogenes from Ruta graveolens Line. Kor J Food Sci Technol 32: 1379-1388
  7. Dural B, Shetty K. 2001. The stimulation of phenolics and antioxidant activity in pea (Pisum sativurn) elicited by genetically transformed andise root extract. J Food Biochem 25: 361-377
  8. Cavidson PH, Parish ME. 1989. Methods of testing the efficacy of food antimicrobials. Food Technol 43: 148-150
  9. Cushman DW, Ondetti MA. 1980. Inhibitors of angiotensin converting enzyme for treatment of hypertension. Biochem Pharm 29: 1871-1877
  10. Stirpe F, Corte ED. 1969. The regulation of rat liver xanthine oxidase. J BioI Chem 244: 3855-3863
  11. Blois MS. 1958. Antioxidant determination by the use of stable free radical. Nature 26: 1198-1199
  12. Pellegrin N, Roberta R, Min Y, Catherine RE. 1998. Screening of diatry carotenoids and carotenoid-rich fruit extracts for antioxidant activites applying 2,2' -azinobis(3-ehylenebenzothiazoline-6-sulfonic acid) radical cation decolorization assay. Method in Enzymol 299: 379-389
  13. Andarwulan N, Shetty K. 1999. Phenolic content in differentiated tissue cultures of untransforned and Agrobacterium-transformed roots of anise (Pimpinella anisum L.) J Agric Food Chem 47: 1776-1780
  14. Buege JA, Aust SD. 1978. Microsomal lipid peroxidation. Method in Enzymol 105: 302-310
  15. Choi HS. 1994. Peroxide and nutrition of lipids. J Kor Soc Technol 28: 867-878
  16. Choi YC, Kim MG, Shin JJ, Park JM, Lee JS. 2003. The antioxidant activities of the some commercial teas. J Korean Soc Food Sci Nutr 32: 723-727
  17. Lee DH, Kim JH, Kim NM, Pack JS, Lee JS. 2002. Manufacture and physiological functionality of Korean traditional liquorny using Paecilomyese japonica. Kor J Mycal 30: 141-146
  18. Yagi K. 1987. Lipid peroxides and human diease. Chem Phys Lipids 45: 337-340
  19. Tarladigis BG, Watts BM, Younathan MT, Dugan LR. 1998. A distillation method for the quantitative determination of malomaldehyde in rancid food. J Am Oil Chem 37: 44-49

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