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Rue(Ruta graveolens L.) 추출물의 Helicobacter pylori에 대한 항균활성과 생리활성효과

Inhibition against Helicobacter pylori and Biological Activities by Rue (Ruta graveolens L.) Extracts

  • 조영제 (상주대학교 식품공학과) ;
  • 천성숙 (영남대학교 식품가공학과) ;
  • 김정환 (상주대학교 식품공학과) ;
  • 윤소정 (상주대학교 식품공학과)
  • Cho, Young-Je (Dept. of Food Engineering, Sangju National University) ;
  • Chun, Sung-Sook (Dept. of Food Science & Technology, Yeungnam University) ;
  • Kim, Jeung-Hoan (Dept. of Food Engineering, Sangju National University) ;
  • Yoon, So-Jung (Dept. of Food Engineering, Sangju National University)
  • 발행 : 2005.04.01

초록

Rue잎을 물과 에탄올로 추출하여 항균활성과 생리활성 효과를 탐색하였다. 그 결과 각 추출물의 총페놀 함량은 물 추출물에서 16.39 mg/g 알콜 추출물에서 17.07 mg/g로 나타났다. Helicobacter pylori에 대한 disc 방법에서 $200\;{\mu}g/mL$의 폴리페놀 첨가 시 물과 알콜 추출물은 각각 12 mm, 13 mm의 직경을 나타내어 항균활성이 있음을 알 수 있었으며, 2-fold dilution 방법으로 항균활성 검증 결과 물 추출물보다 알콜 추출물에서 더 높은 항균활성을 나타내었다. 물과 알콜 각 추출물의 ABTS radical decolorlization과 antioxidant protection factor(PF)를 살펴 본 결과 ABTS는 물 추출물이 약 $96\%$로 높은 저해율을 나타내었으며 PF는 알콜 추출물에서 1.2로 물 추출물 0.8보다 높게 나타났다. DPPH에 대한 전자공여능은 물 추출물이 89.38로 알콜 추출물 $86.90\%$보다 높게 나타났으며 활성 산소중 지방산화를 일으키는데 주요한 역할을 하는 hydroxyl radical에 대한 각 추출물의 영향은 알콜 추출물이 binding능력이 탁월하여 물 추출물에 비해 낮은 TBARS 값을 나타내었다. Angiotensin converting enzyme과 xanthine oxidase 저 해 활성은 알콜 추출물이 물 추출물보다 더 높게 나타났고, HPLC에 의한 페놀 분석 결과 rosemarinic acid의 함량이 알콜 추출물에서 가장 많았으며 다음으로 qurcetin 함량이 높게 나타났다.

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