Antioxidative effects of traditional medicinal plants on lipid peroxidation

지질 과산화에 대한 전통약용 식물의 항산화 효과

  • Hah, Dae-sik (Gyeongnam Provincial Government Institute of Health and Environment) ;
  • Kim, Chung-hui (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Kim, Gon-sup (Collage of Veterinary Medicine and Institute of Animal Science, Gyeongsang National University) ;
  • Kim, Eui-gyung (Collage of Veterinary Medicine and Institute of Animal Science, Gyeongsang National University) ;
  • Kim, Jong-shu (Collage of Veterinary Medicine and Institute of Animal Science, Gyeongsang National University)
  • 하대식 (경남 보건환경 연구원) ;
  • 김충희 (진주산업대학교 동물생명과학과) ;
  • 김곤섭 (경상대학교 수의과대학(동물의학연구소)) ;
  • 김의경 (경상대학교 수의과대학(동물의학연구소)) ;
  • 김종수 (경상대학교 수의과대학(동물의학연구소))
  • Accepted : 2005.08.22
  • Published : 2005.09.22

Abstract

To assess the antioxidative activity of 12 medicinal plants on lipid peroxidation, twelves traditional medicinal plants extracted with 95% methanol were investigated the antioxidative activity using DPPH, thiocyanate acid method, and thiobarbituric acid (TBA) methods. Out of 12 medicinal plants extracted with methanol, the extraction yields of Sedum kamtschaticum was the highest values (49.46%) among them and Geranicum sibiricum, Saururus chinensis root (R), Agrimonia pilosa leaf (L), Agrimonia pilosa root was the lowest value (9.97%). Radical scavenging effect of the selected traditional medicinal plants extracted from different extract solution were examined by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical method. Antioxidative activity of methanolic extracts was higher than those of ethanol and n-hexane extracts. Scavenging effects in Sedum kamtaschaticum (R) determined by DPPH radical showed the highest among the 12 plants. The antioxidative effects of the first four medicinal plants were similar to those of butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT), but higher than that of tocopherol, which was used as a handled control. Antioxidative effects of each indicated concentration of the methanolic extracts on linoleic acid by thiocyanate method was the highest in Sedum kamtschaticum and followed by Geum japonicum and Agrimonia pilosa and their antioxidative effect were similar to those of BHA, and BHT, but higher than that of tocopherol. Antioxidative effects of the selected medicinal methanolic extract on linoleic acid by thiocyanate acid method were examined for 15 days. Peroxidation of control and tocopherol group occurred on days 5 and 9, respectively, but BHA, BHT, selected medicinal methanolic extract group did not occur until on day 15. Antioxidative effects of the selected medicinal methanolic extract on linoleic acid by TBA method were examined for 15 days. Antioxidative activity was similar to those obtained by thiocyanate acid method.

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