Effects of conjugated linoleic acid and selenium on body composition and serum cholesterol concentration in broiler chickens

CLA와 selenium의 투여가 닭의 체구성과 혈중 콜레스테롤 수준에 미치는 영향

  • 전호진 (건국대학교 수의과대학) ;
  • 김진영 (건국대학교 수의과대학) ;
  • 임좌진 (건국대학교 수의과대학) ;
  • 이수한 (건국대학교 수의과대학) ;
  • 이병한 (건국대학교 수의과대학) ;
  • 정순욱 (건국대학교 수의과대학) ;
  • 박희명 (건국대학교 수의과대학) ;
  • 정병현 (건국대학교 수의과대학)
  • Accepted : 2005.08.17
  • Published : 2005.09.22


The purpose of this study was to examine whether conjugated linoleic acid and selenium supplementation in broiler chicken diets would be effective, enhance indices of immune status and body weight, and modulate serum lipid concentration. Forty Hyline brown chickens, 1 weeks of age, were divided into 5 groups of 8 chickens. Chickens were fed the experimental diets supplemented with 1% CLA (conjugated linoleic acid; Group 1), 1% CLA + selenium (Group 2), 1% safflower-seed-oil as LA (Group 3), 1% safflower-seed-oil as LA + selenium (Group 4) or nothing (Control) for 4 weeks. After 4 weeks, serum, liver, spleen and abdominal fat were taken. Measurement of total immunoglobulin were executed using sandwich ELISA. Weight ratio of liver to body showed that the group fed with CLA were significantly higher than the group fed with CLA + selenium. Weight ratios of spleen and fat to body showed no significantly differences. In concentrations of serum total cholesterol and HDL-cholesterol, the group fed with CLA showed significantly higher values than that fed with CLA + selenium. In concentrations of serum triglyceride and LDL-cholesterol there were no significantly differences between the treatment groups. In conclusion, supplementation of CLA with selenium protected hepatomegaly and reduced level of serum total cholesterol and HDL-cholestererol in chickens.


Supported by : 건국대학교


  1. Badinga L, Selberg KT, Dinges AC, Corner CW, Miles RD. Dietary conjugated linoleic acid alters hepatic lipid content and fatty acid composition in broiler chickens. Poult Sci 2003, 82, 111-116 https://doi.org/10.1093/ps/82.1.111
  2. Belury MA, Kempa-Steczko A. Conjugated linoleic acid modulates hepatic lipid composition in mice. Lipids 1997, 32, 199-204 https://doi.org/10.1007/s11745-997-0025-0
  3. Belury MA, Moya-Camarena SY, Liu K, Heuvel JPV. Dietary conjugated linoleic acid induces peroxisome specific enzyme accumulation and ornithine decarboxylase activity in mouse liver. J Nutr Biochem 1997, 8, 579-584 https://doi.org/10.1016/S0955-2863(97)00093-4
  4. Benton D, Cook R. Selenium supplementation improves mood in a double-blind crossover trial. Biol Psychiatry 1991, 29, 1092-1098 https://doi.org/10.1016/0006-3223(91)90251-G
  5. Cherian G, Holsonbake TB, Goeger MP, Bildfell R. Dietary CLA alters yolk and tissue FA composition and hepatic histopathology of laying hens. Lipids 2002, 37, 751-757 https://doi.org/10.1007/s11745-002-0957-4
  6. Chi H, Thomas Y, Lin Y, Yu H, Jun W, Zhen-Yu C. Dietary conjugated linoleic acid mixture affects the activity of intestinal acyl coenzyme A: cholesterol acyltransferase in hamsters. Br J Nutr 2000, 84, 935-941
  7. Clark LC, Combs GF Jr, Turnbull BW. Effects of selenium supplementation for cancer prevention in patients with carcinoma of the skin. A randomized controlled trial. Nutritional Prevention of Cancer Study Group, JAMA 1996, 276, 1957-1963 https://doi.org/10.1001/jama.276.24.1957
  8. Clark LC, Combs GFJr, Turnbull BW. Effects of selenium supplementation for cancer prevention in patients with carcinoma of the skin. A randomized controlled trial. Nutritional Prevention of Cancer Study Group, Erratum in: JAMA 1997, 277, 1520 https://doi.org/10.1001/jama.1997.03540430032028
  9. Cook ME, Miller CC, Pariza MW. Immune modulation by altered nutrient metabolism: nutritional control of immune-induced growth depression. Poultry Sci. 1993, 72, 1301-1305 https://doi.org/10.3382/ps.0721301
  10. de Deckere EAM, van Amelsvoort JMM, McNeil GP, Jones P. Effects of conjugated linoleic acid (CLA) isomers on lipid levels and peroxisome proliferation in the hamster. Br J Nutr 1999, 82, 309-317
  11. Decker EA. The Pole of phenolics, conjugated linoleic acid, carnosine, and pyrroloquinoline quinone as nonessential dietary Antioxidants. Nutr Rev 1995, 53, 49-58 https://doi.org/10.1111/j.1753-4887.1995.tb01502.x
  12. Delany JP, Blohm F, Truett AA, Scimeca JA, West DB. Conjugated linoleic acid rapidly reduces body fat content in mice without affecting energy intake. Am J Physiol 1999, 276, R1172-R1179
  13. Du M, Ahn DU. Effect of dietary conjugated linoleic acid on the growth rate of live birds and on the abdominal fat content and quality of broiler meat. Poult Sci 2002, 81, 428-433 https://doi.org/10.1093/ps/81.3.428
  14. Dugan ME, Aalhus, JL, Schaefer AL, Kramer JKG. The effect of conjugated linoleic acid on fat to lean repartitioning and feed conversion in pigs. Can J Ani Sci 1997, 77, 723-725 https://doi.org/10.4141/A97-084
  15. Finley JW, Penland JG. Adequacy or deprivation of dietary selenium in healthy men: clinical and psychological findings. J Trace Elem Exp Med 1998, 11, 11-27 https://doi.org/10.1002/(SICI)1520-670X(1998)11:1<11::AID-JTRA3>3.0.CO;2-6
  16. Ha YL, Grimm NK, Pariza MW. Anticarcinogens from fried ground beef : hear-altered derivatives of linoleic acid. Carcinogenesis 1987, 8, 1881-1887 https://doi.org/10.1093/carcin/8.12.1881
  17. Ha YL, Storkson J, Pariza MW. Inhibition of benzo(a)pyrene - induced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid. Cancer Res 1990, 50, 1097-1101
  18. Hawkes WC, Hornbosterl L. Effects of dietary selenium on mood in healthy men living in a metabolic research unit. Biol Psychiatry 1996, 39, 121-128 https://doi.org/10.1016/0006-3223(95)00085-2
  19. Hayek MG, Han SN, Wu D, Watkins BA, Meydani M, Dorsey JL, Smith DE, Meydani SN. Dietary conjugated linoleic acid influences the immune response of young and old C57BL/6NCrlBR mice. J Nutr 1999, 129, 32-38
  20. Houseknecht KL, Vanden Heuvel JP, Moya-Camarena SY, Portocarrero CP, Peck LW, Nickel KP, Belury MA. Dietary conjugated linoleic acid normalizes impaired glucose tolerance in zucker diabetic fatty fa/fa Rat. Biochem Biophys Res Comm 1998, 244, 678-682 https://doi.org/10.1006/bbrc.1998.8303
  21. Hughes PE, Hunter WJ, Tove SB. Biohydrogenation of unsaturated fatty acids. J Biol Chem 1982, 257, 3643-3649
  22. Ip C, Chin SF, Scimeca JA, Pariza MW. Mammary cancer prevention by conjugated dienoic derivative of linoleic acid. Cancer Res 1991, 51, 6118-6124
  23. Peters JM, Park YH, Gonzalez FJ, Pariza MW. Influence of conjugated linoleic acid on body composition and target gene expression in peroxisome proliferator-activated receptor ${\alpha}$--nell mice. Biochim Biophys Acta. 1533. 2001, 233-242
  24. Kang BP, Mehta U, Bansal MP. Selenium supplementation protects from high fat diet-induced atherogenesis in rats: role of mitogen stimulated lymphocytes and macrophage NO production. Indian J Exp Biol 2001, 39, 793-797
  25. Kelley DS, Taylor PC, Rudolph IL, Benito P, Nelson GJ, Mackery BE, Erickson KL. Daitary conjugated linoleic acid did not alter immune status in young healthy women. Lipids 2000, 35, 1065-1071 https://doi.org/10.1007/s11745-000-0620-0
  26. Khan MZ, Szarek J, Markiewicz K. Effects of oral administration of toxic levels of lead and selenium upon concentration of different elements in the liver of broiler chicks. Zentralbl Veterinarmed 1993, 40, 652-664 https://doi.org/10.1111/j.1439-0442.1993.tb00681.x
  27. Lee KN, Kritchevsky D, Pariza MW. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis. 1994, 108, 19-25 https://doi.org/10.1016/0021-9150(94)90034-5
  28. Liew C, Schut HA, Chin SF, Pariza MW, Dashwood RH. Protection of conjugated linoleic acids against 2-amino-3-methlimidazo [4,5-f] quinoline-induced colon carcinogenesis in the F344 rat: a study of inhibitory mechanisms. Carcinogenesis 1995, 16, 3037-3043 https://doi.org/10.1093/carcin/16.12.3037
  29. Mir Z, Rushfeldt ML, Mir PS, Paterson LJ, Weselake RJ. Effect of dietary supplementation with either conjugated linoleic acid (CLA) or linoleic acid rich oil on the CLA content of lamb tissue. Small Rumin Res 2000, 36, 25-31 https://doi.org/10.1016/S0921-4488(99)00087-5
  30. Neve J. Selenium as a risk factor for cardiovascular diseases. J Cardiovasc Risk 1996, 3, 42-47 https://doi.org/10.1097/00043798-199602000-00006
  31. Olivieri O, Girelli D, Azzini M. Low selenium status in the elderly influences thyroid hormones. Clin Sci. 1995, 89, 637-642 https://doi.org/10.1042/cs0890637
  32. Pariza MW, Park Y, Cook ME. The biologically active isomers of conjugated linoleic acid. Prog Lipid Res 2001, 40, 283-298 https://doi.org/10.1016/S0163-7827(01)00008-X
  33. Park Y, Albright KJ, Liu W, Storkson JM, Cook ME, Pariza MW. Effect of conjugated linoleic acid on body composition changes in mice. Lipids 1997, 32, 853-858 https://doi.org/10.1007/s11745-997-0109-x
  34. Park Y, Storkson JM, Albright KJ, Liu W, Pariza MW. Evidence that the trans-10, cis-12 isomer of conjugated linoleic acid induces body composition changes on mice. Lipids 1999, 34, 235-241 https://doi.org/10.1007/s11745-999-0358-8
  35. Roderick CM, Teresa SR, Geoffrey JB. Selenium : an essential element for immune function. Immunol Today 1998, 19, 342-345 https://doi.org/10.1016/S0167-5699(98)01294-8
  36. Roy M, Kiremidjian-Schumacher L, Wishe HI. Supplementation with selenium restores age-related decline in immune cell function. Proc Soc Exp Biol Med 1995, 209, 369-375
  37. Scimeca JA, Miller GD. Potential health benefits of conjugated linoleic acid. J Am Coll Nutr 2000, 19, 470S-471S https://doi.org/10.1080/07315724.2000.10718949
  38. Sugano M, Tsujita A, Yamasaki M, Noguchi M, Yamada K. Conjugated linoleic acid modulates tissue levels of chemical mediators and immunoglobulins in rats. Lipids 1998, 33, 521-527 https://doi.org/10.1007/s11745-998-0236-4
  39. Swain BK, Johri TS, Majumdar S. Effect of supplementation of vitamin E, selenium and their different combinations on the performance and immune response of broilers. Br Poult Sci 2000, 41, 287-292 https://doi.org/10.1080/713654938
  40. Swecker WS, Thatcher CD, Eversole DE, Blodgett DJ, Schurig GG. Effect of selenium supplementation on colostral IgG concentration in their calves. Am J Vet Res 1995, 56 450-453
  41. Swecker WS, Eversole DE, Thatcher CD, Blodgett DJ, Schurig GG, Meldrum JB. Influence of supplemental selenium on humoral immune responses in weaned beef calves. Am J Vet Res 1989, 50, 1760-1763
  42. Szymczyk B, Pisulewski PM, Szczurek W, Hanczakowski P. Effects of conjugated linoleic acid on growth performance, feed conversion efficiency, and subsequent carcass quality in broiler chickens. Br J Nutr 2001, 85, 465-473 https://doi.org/10.1079/BJN2000293
  43. Taylor EW, Nadimpalli RG, Ramanathan CS. Genomic structures of viral agents in relation to the biosynthesis of selenoproteins. Biol Trace Elem Res 1997, 56, 63-91 https://doi.org/10.1007/BF02778984
  44. Tsuboyama-Kasaoka N, Takahashi M, Tanemura K, Kim H, Tange T, Okauyama H, Kasai M, Ikemoto S. Ezaki O. Conjugated linoleic acid supplementation reduce adipose tissue by apoptosis and develos lipodystrophy in nice. Diabetes 2000, 49, 1534-1542 https://doi.org/10.2337/diabetes.49.9.1534
  45. Underwood EJ. Trace elements in human and animal nutrition, 4th ed, pp. 303-345, Academic Press, New York, 1977
  46. West DB, Delany JP, Camet PM, Blohm F, Truett AA, Scimeca J. Effects of conjugated linoleic acid on body fat and energy metabolism in the mouse. Am J Physiol 1998, 275, R667-R672
  47. Yuguang L, Arja K, Johan AE, Schuurbiers RD. Different effects of conjugated linoleic acid isomers on lipoprotein lipase activity in 3T3 - 11 adipocytes, J Nutr Biochem 2001, 12, 183-189 https://doi.org/10.1016/S0955-2863(00)00155-8