Effect of Allopurinol on Vascular Endothelial Cells Damaged by Hydrogen Peroxide In Vitro

Hydrogen Proxide에 의해 손상된 배양 혈관내피세포에 대한 Allopurinol의 영향

  • Suk, Seung-Han (Department of Neurology, Wonkwang University Sanbon Medical Center)
  • 석승환 (원광대학교 의과대학 산본병원 신경과학교실)
  • Published : 2006.08.25

Abstract

In order to examine the effect of oxygen free radicals on the vascular endothelial cells, cell viability was measured by XTT assay after bovine pulmonary vascular endothelial cell line(BPVEC) was treated only with hydrogen peroxide. In addition, the antioxidant effect of allopurinol on cells treated with hydrogen peroxide was examined by colormetric assay. in this study, the BPVEC treated with hydrogen peroxide showed the significantly decreased cell viability compared with control. Whereas, the viability of cells treated with hydrogen peroxide and allopurinol has significantly increased when compared with that of cells treated only with hydrogen peroxide. These results suggested that hydrogen peroxide, one of the oxygen free radicals showed cytotoxic effect and allopurinol has protective effect on oxygen free radical-induced cytotoxicity.

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