Antioxidative and Antimicrobial Activities of Euphorbia jolkini Extracts

암대극(Euphorbia jolkini Boiss) 추출물의 항산화 및 항균활성

  • Kim, Ji-Young (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute) ;
  • Lee, Jung-A (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute) ;
  • Yoon, Weon-Jong (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute) ;
  • Oh, Dae-Ju (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute) ;
  • Jung, Yong-Hwan (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute) ;
  • Lee, Wook-Jae (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute) ;
  • Park, Soo-Yeong (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute)
  • 김지영 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 이정아 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 윤원종 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 오대주 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 정용환 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 이욱재 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 박수영 ((재)제주하이테크산업진흥원 제주생물종다양성연구소)
  • Published : 2006.10.01


The antioxidative and antimicrobial activities of Euphorbia jolkini extracts were investigated. Total polyphenohc compounds extracted were approximately as follows: 162.08 mg/g from ethanol, 12.64 mg/g from n-hexane, 48.11 mg/g from dichloromethane, 544.08 mg/g from ethyl acetate, 176.42 mg/g from butanol, and 30.00 mg/g from water. The ethylacetate fraction of this extraction showed the highest antioxidative activity $(IC_{50})$: DPPH radical scavenging capacity was measured at $8.38\;{\mu}g/mL$, xanthine oxidase inhibitory activity was $466.01\;{\mu}g/mL$, superoxide radical scavenging capacity was $11.39\;{\mu}g/mL$, and nitric oxide scavenging capacity was $332.11\;{\mu}g/mL$. Antimicrobial activities were determined by paper disc method and minimum inhibitory concentration of E. jolkini extracts against food-borne pathogens and spoilage bacteria. The growth inhibition curves of E. jolkini extracts against Bacillus cereus, Listeria monocytogenes, and Escherichia coli were also determined. These results suggest that the ethylacetate fraction of E. jolkini has strong antimicrobial activity against the all species of microorganisms as well as strong antioxidant activity.


  1. Kim SM, Cho YS, Sung SK. The antioxidant ability and nitrite scavenging ability of plant extracts. Korean J. Food Sci. Technol. 33: 626-632 (2001)
  2. Seo KL, Kim DY, Yang SI. Studies on the antimicrobial effect of wasabi extracts. Korean J. Nutr. 28: 1073-1077 (1995)
  3. Park UK, Chang DS, Cho HR. Screening of antimicrobial activity for medicinal herb extracts. J. Korean Soc. Food Nutr. 21: 91-96 (1992)
  4. Hahn YS. Antimicrobial effects of Camellia janponica L. leaves extracts on food-borne pathogenic microorganisms. Korean J. Food Sci. Technol. 37: 113-121 (2005)
  5. Lee TB. Illustrated Flora of Korea. Hyangmoon Publishing Co., Seoul, Korea. p. 512 (1999)
  6. Gutfinger T. Polyphenols in olive oils. J. Am. Oil. Chem. Soc. 58: 966-968 (1981)
  7. Cheng ZJ, Kuo SC, Chan SC, Ko FN, Teng CM. Antioxidant properties of butein isolated from Dalbergia odorifera. Biochim. Biophys. Acta. 1392: 291-299 (1998)
  8. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS. Tannenbaum SR. Analysis of nitrate, nitrite, and (15N) nitrate in biological fluids. Anal. Biochem. 126: 131-136 (1982)
  9. Marcocci L, Maguire JJ, Droy-Lefaix MT, Packer L. The nitric oxide-scavenging properties of ginkgo biloba extract EGb 761. Biochem. Biophys. Res. Commun. 201: 748-755 (1994)
  10. Amsterdam D. Susceptibility testing of antimicrobial in liquid media. Antibiotics in Laboratory Medicine. Williams and Wikins, MD, USA. pp. 52-111 (1996)
  11. Whang HJ, Han WS, Yoon KR. Quantitative analysis of total phenolic content in apple. Anal. Sci. Technol. 14: 377-383 (2001)
  12. Davies KJA. Free radicals and oxidative stress environment. pp. 1-31. In: Drugs and Food Additives. Rice-Evans C, Halliwell B, Lunt GC (eds). Portland Press, Portland (1995)
  13. Ahn BT, Lee BT, No JS, Lee GS. Phenolic compounds from Euphorbia ebracteolata. Kor. J. Pharmacogn. 27: 136-141 (1996)
  14. Harman D. Free radicals in Biology V. Acemic Press. New York, USA. pp. 255-275 (1982)
  15. Ames BN. Dietary carcinogens and anticarcinogens, oxygen radicals and degenerative diseases. Science 221: 1256-1264 (1983)
  16. Lee CY, Oh SW, Hong HD. Antsmicrobial characteristics of edible medicinal herbs extracts. Korean J. Food Sci. Technol. 34: 700-709 (2002)
  17. Blois MS. Antioxidant determination by the use a stable free radical. Nature 26: 1199-1200 (1958)
  18. Hatano T. Constituents of natural medicines with scavenging effects on active oxygen species-tannins and related polyphenols. Natural Medicines 49: 357-363 (1995)
  19. Yang MS, Ha YL, Nam SH, Choi SU, Jang DS. Screening of domestic plants with antibacterial activity. J. Korean Soc. Appl. Bio. Chem. 38: 584-589 (1995)
  20. Park SH, Kim JA, Hua XG, Lee CG, Choi JY. Isolation of 5${\alpha}$-reductase Inhibitors from Euphorbia jolkinii. Korean J. Pharmacogn. 36: 9-16 (2005)
  21. Shin DH, Kim MS, Han JS. Antimicrobial effect of ethanol extracts from some medicinal herbs and their fractionates against food-born bacteria. Korean J. Food Sci. Technol. 29: 808-816 (1997)
  22. Jeon YO, Kim SI, Han YS, Kim KH. Screening of antimicrobial activity of the Plantain (Plantago asiatica) extracts. Korean J. Soc. Food Sci. 14: 498-502 (1998)
  23. Kim JJ, Lee JS, Kim SY, Kim JA. Inhibitory effect of hydrolysable tannins isolated from the Euphorbia helioscopia on mushroom tyrosinase activity in vitro. Yakhak Haeji 45: 214-219 (2001)
  24. Lee SH, Lim YS. Antimicrobial effects of Schizandra chinensis extracts on pathogenic microorganisms. J. Korean Soc. Food Sci. Nutr. 27: 239-243 (1998)
  25. Masaki H, Sakaki S, Atsumi T, Sakurai H. Active oxygen scavenging activity of plant extracts. Biol. Pharm. Bull. 18: 162-166 (1995)
  26. Bae JH. Effect of Artemisia capillaries extracts on the growth of food-borne pathogens. Korean J. Nutr. 36: 147-153 (2003)
  27. Tabance N, kirimer N, Demirci B, Demirci F, Baser KHC. Composotion and antimicrobial activity of the essential oils of Micromeria cristata subsp. Phrygia and enantiomeric distribution of borneol. J. Agric. Food Chem. 49: 4300-4303 (2001)
  28. Koh JH, Hwang MO, Moon SJ, Hwang SY, Son JY, Antioxidative and antimicrobial activities of pomegranate seed extracts. Korean J. Food Cookery Sci. 21: 171-178 (2005)
  29. Ahn BT, Kang SS. Phenolic compounds from aerial parts of Euphorbia pekinensis (II). Korean J. Pharmacogn. 27: 142-145 (1996)
  30. Aruoma OI. Nutrition and health aspects of free radicals and antioxidants. Fd. Chem. Toxicol. 32: 671- 683 (1994)
  31. Kim HJ, Jun BS, Kim SK, Cha JY, Cho YS. Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and flower of safflower (Carthamus tinctiorius L.). J. Korean Soc. Food Sci. Nutr. 29: 1127-1132 (2000)