Induction of Quinone Reductase Activity in Hepatoma Cells by Paprika (Capsicum annuum L.)

파프리카 추출물이 quinone reductase 유도활성에 미치는 영향

  • Yu, Mi-Hee (Department of Food Science and Technology, Keimyung University,The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Lee, Hyo-Jung (Department of Food Science and Technology, Keimyung University,The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Im, Hyo-Gwon (Department of Food Science and Technology, Keimyung University,The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Lee, Syng-Ook (Department of Food Science and Technology, Keimyung University,The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Lee, In-Seon (Department of Food Science and Technology, Keimyung University,The Center for Traditional Microorganism Resources, Keimyung University)
  • 유미희 (계명대학교 식품가공학,계명대학교 전통미생물자원개발 및 산업화연구(TMR) 센터) ;
  • 이효정 (계명대학교 식품가공학,계명대학교 전통미생물자원개발 및 산업화연구(TMR) 센터) ;
  • 임효권 (계명대학교 식품가공학,계명대학교 전통미생물자원개발 및 산업화연구(TMR) 센터) ;
  • 이승욱 (계명대학교 식품가공학,계명대학교 전통미생물자원개발 및 산업화연구(TMR) 센터) ;
  • 이인선 (계명대학교 식품가공학,계명대학교 전통미생물자원개발 및 산업화연구(TMR) 센터)
  • Published : 2006.10.01


Phase 2 enzymes are transcriptionally induced by a wide variety of chemical agents and natural products, and their induction plays a critical role in protection against chemical carcinogens and other toxic xenobiotics. The activity of the methanol extract and fractions of paprika (Capsicum annuum L.) was examined in murine Hepa1c1c7 cells for the induction of nicotinamide adenine dinucleotide (phosphate) NAD(P)H/quinone reductase (QR). The ethyl acetate (EtOAc) fraction induced QR activity in a dose-dependent manner in the concentration range of 10 to $500\;{\mu}g/mL$ with a maximum of a 3.3-fold increase in induction. The EtOAc fraction also showed high QR induction potency in Ah-receptor-defective mutant of Hepa 1c1c7 cells ($BP^rcl$ cells), which indicates that this fraction is a monofunctional inducer of QR. These results suggest that useful cancer chemopreventive materials could be isolated from EtOAc fraction of Paprika.


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