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Effect of Types of Egg Shell Calcium Salts and Egg Shell Membrane on Calcium Metabolism in Rats

난각 칼슘염의 종류와 난막의 존재유무가 흰쥐의 칼슘대사에 미치는 영향

  • Noh, Kyung-Hee (School of Food and Life Sciences, Food Science Institute, and Biohealth Product Research Center, Inje University) ;
  • Lee, Sang-Hyun (School of Food and Life Sciences, Food Science Institute, and Biohealth Product Research Center, Inje University) ;
  • Ma, Jie (School of Food and Life Sciences, Food Science Institute, and Biohealth Product Research Center, Inje University) ;
  • Zhou, Yusi (School of Food and Life Sciences, Food Science Institute, and Biohealth Product Research Center, Inje University) ;
  • Kim, Jae-Cherl (School of Food and Life Sciences, Food Science Institute, and Biohealth Product Research Center, Inje University) ;
  • Kim, Myo-Jeong (School of Food and Life Sciences, Food Science Institute, and Biohealth Product Research Center, Inje University) ;
  • Song, Young-Sun (School of Food and Life Sciences, Food Science Institute, and Biohealth Product Research Center, Inje University)
  • 노경희 (인제대학교 의생명공학대학 식품생명과학부, 식품과학연구소 및 바이오헬스소재 연구센터) ;
  • 이상현 (인제대학교 의생명공학대학 식품생명과학부, 식품과학연구소 및 바이오헬스소재 연구센터) ;
  • ;
  • ;
  • 김재철 (인제대학교 의생명공학대학 식품생명과학부, 식품과학연구소 및 바이오헬스소재 연구센터) ;
  • 김묘정 (인제대학교 의생명공학대학 식품생명과학부, 식품과학연구소 및 바이오헬스소재 연구센터) ;
  • 송영선 (인제대학교 의생명공학대학 식품생명과학부, 식품과학연구소 및 바이오헬스소재 연구센터)
  • Published : 2006.08.30

Abstract

This study was carried out to investigate the effect of egg shell calcium salt types and egg shell membrane on calcium metabolism in rats. Sprague-Dawley male rats, 4 weeks of age, were fed on free-calcium diets for 2 weeks after adjustment period. Rats weighing approximately $247{\pm}2.3g$ were divided into 6 groups and were fed on the experimental diets containing 0.2% calcium for 4 weeks. Experimental groups were as follows; {ES(M+)} (egg shell powder diet with egg shell membrane), {ES(M-)} (egg shell powder diet without egg shell membrane), {AC(M+)} (egg shell calcium acetate diet with egg shell membrance), {AC(M-)} (egg shell calcium acetate diet without eg shell membrane), {GC(M+)} (egg shell calcium glucuronate diet with egg shell membrane) and {GC(M-)} (egg shell calcium glucuronate diet without egg shell membrane). Bone length of femur was significantly different by the types (p<0.05) of egg shell calcium salts. Bone mineral density of femur showed the highest level in AC(M-) group. Calcium content of femur and calcium absorption rate were higher in egg shell calcium salt groups than in eg shell powder groups. Calcium absorption rate and retention were significantly different (p<0.05) among the types of eg shell calcium salts and were higher in the AC(M-) group than in the other groups. Alkaline phosphatase activity, parathyroid hormone and osteocalcin levels of serum showed no significant difference among the experimental groups. From the above results, it is concluded that bioavailability of calcium is higher in groups of egg shell calcium salts compared to those in egg shell powder, even though egg shell membrane has no effect on calcium metabolism. Thus, these findings suggest the possibility of using egg shell calcium salts as a functional food material related to calcium metabolism.

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