구강편평세포암종에서의 Cisplatin 유도 아폽토시스에서의 NF-$\kappa$B의 활성화

  • Seo, Jong-Chun (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University) ;
  • Sung, Iel-Yong (Dept. of Oral & Maxillofacial Surgery, Medical College of Ulsan University) ;
  • Kim, Jong-Roul (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University)
  • 서종천 (부산대학교 치과대학 구강악안면외과학교실) ;
  • 성일용 (울산대학교 의과대학 울산대학교병원 치과/구강악안면외과) ;
  • 김종렬 (부산대학교 치과대학 구강악안면외과학교실)
  • Published : 2006.04.30


Purpose: This study was done to confirm the role of NF-$\kappa$B in cisplatin-induced apoptosis of oral squamous cell carcinoma. Materials and Methods: Five cell lines originated from different oral cancer patients were tested for the apoptosis by the treatment of cisplatin. These cells showed different degree of cisplatin-resistance and the order is OSCC-2>OSCC-3>OSCC-5> OSCC-1>OSCC-4. OSCC-2 and OSCC-4 cells were assayed for the apoptosis by measuring DNA fragmentation and TUNEL staining after cisplatin treatment. While OSCC-4 cells showed apoptosis, OSCC-2 cells showed no or very slight apoptosis by cisplatin treatment. Next, It was determined whether NF-$\kappa$B activation is required in mediating cisplatin-induced apoptosis of OSCC-4. Result: The result was that elevated NF-$\kappa$B activity mediated cisplatin-induced apoptosis. Conclusion: In conclusion, these findings suggest that NF-$\kappa$B activation is essential to cisplatin-induced apoptosis and it may be involved in cisplatin resistance in OSCC cells.


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