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Antimutagenic and Cytotoxic Effects of Fagopyrum esculentum Moenech Noodles Extracts

메밀 국수 추출물의 항돌연변이원성 및 세포독성 효과

  • Yoo, Kwang-Ha (School of Bioscience and Biotechology, Kangwan National University) ;
  • Kim, Soo-Hyun (School of Bioscience and Biotechology, Kangwan National University) ;
  • Ham, Young-An (School of Bioscience and Biotechology, Kangwan National University) ;
  • Yoo, Soo-Jung (School of Bioscience and Biotechology, Kangwan National University) ;
  • Oh, Hyun-Taek (School of Bioscience and Biotechology, Kangwan National University) ;
  • Ham, Seung-Shi (School of Bioscience and Biotechology, Kangwan National University)
  • 유광하 (강원대학교 바이오산업공학부) ;
  • 김수현 (강원대학교 바이오산업공학부) ;
  • 함영안 (강원대학교 바이오산업공학부) ;
  • 유수정 (강원대학교 바이오산업공학부) ;
  • 오현택 (강원대학교 바이오산업공학부) ;
  • 함승시 (강원대학교 바이오산업공학부)
  • Published : 2006.12.29

Abstract

This study was performed to determine the antimutagenic and anticancer effects of Fagopyrum esculentum Moenech noodles (FEMN) extracts using Ames test and cytotoxicity, respectively. FEMN made buckwheat wet noodles (BWN), buckwheat extruded noodles (BEN) and buckwheat dehydrated noodles (BDN) by 60% buckwheat flour and 70% buckwheat flour. The inhibitory effects of FEMN extracts on cell proliferation in A549, Hep3B, MCF-7, AGS and HeLa were investigated by SRB assay. The cytotoxic effects of FEMN against the cell lines with human lung carcinoma (A549), human gastric carcinoma (AGS), human hepatocellular carcinoma (Hep3B), human cervical adenocarcinoma (HeLa) and human breast adenocarcinoma (MCF-7) were inhibited with the increase of the extract concentration. The treatment of 1.0 mg/mL FEMN of 60% BEN extracts showed strong cytotoxicities of 74.7%, 75.3% and 70.5% against AGS, A549 and HeLa, respectively. The inhibition rate of 70% BWN of FEMN extracts in the S. Typhimurium TA100 strain showed 41% against the mutagenesis induced by MNNG. The inhibition rate of 70% BEN of FEMN extracts in the S. Typhimurium TA98 strain showed 45% against the mutagenesis induced by 4NQO.

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