Effects of a Herbal Composition (HemoHIM) on the Activation of Dendritic Cells

생약복합조성물(HemoHIM)의 수지상세포 활성화 효과

  • Shin, Sung-Hae (Dept. of Biology, College of Natural Science, Sunchon National University) ;
  • Kim, Do-Soon (Dept. of Biology, College of Natural Science, Sunchon National University) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University) ;
  • Jo, Sung-Kee (Radiation Food and Biotechnology Team, Advanced Radiation Technology Institute, Jeongeup Campus of Korea Atomic Energy Research Institute) ;
  • Byun, Mung-Woo (Radiation Food and Biotechnology Team, Advanced Radiation Technology Institute, Jeongeup Campus of Korea Atomic Energy Research Institute) ;
  • Yee, Sung-Tae (Dept. of Biology, College of Natural Science, Sunchon National University)
  • 신성해 (순천대학교 생물학과) ;
  • 김도순 (순천대학교 생물학과) ;
  • 김성호 (전남대학교 수의학과) ;
  • 조성기 (한국원자력연구소 방사성연구원 방사선식품생명공학팀) ;
  • 변명우 (한국원자력연구소 방사성연구원 방사선식품생명공학팀) ;
  • 이성태 (순천대학교 생물학과)
  • Published : 2006.12.29


In our previous study, a novel herb mixture (HIM-I) of Angelica gigas radix, Cnidium officinale rhizoma, and Paeonia japonica radix was developed to protect= the intestinal and immune systems and to promote their recovery from radiation damage. A new herbal composition (HemoHIM) with the high immune modulating activity was developed from HIM-I. In the present study, we examined the effects of HemoHIM on the maturation process of murine bone marrow (BM)-derived dendritic cells (DC). BM cells were cultured in the presence of iL-4 and GM-CSF and the generated immature DC were stimulated with HemoHIM for 24 hours. HemoHIM significantly enhanced the expression of co-stimulatory molecules, CD80 and CD86, especially. The activation capacity of HemoHIM-treated DC was significantly higher than that of immature DC, as analyzed by IL-2 and $IFN-\gamma$ production and proliferation of the responding T cells in the co-culture with allogeneic T cells. The antigen-presenting capacity of HemoHIN-treated DC was also increased by the co-culture with OVA-specific T cells (HS-1), as analyzed by IL-2 and $IFN-\gamma$ production and the proliferation. These results indicate that HemoHIM causes the maturation and ;Activation of DC, which may be a part of mechanisms of immunomodulation by HemoHIM.


  1. Hart DN. 1997. Dendritic cells: Unique leukocyte populations which control the primary immune response. Blood 90: 3245-3287
  2. Banchereau J, Briere F, Caux C, Davoust J, Lebecque S, Liu YJ, Pulendran B, Palucka K. 2000. Immunobiology of dendritic cells. Annu Rev Immunol 18: 767-811
  3. Schuler G, Schuler-Thurner B, Steinman RM. 2003. The use of dendritic cells in cancer immunotherapy. Current Opinion in Immunology 15: 138-147
  4. Banchereau J, Steinman RM. 1998. Dendritic cells and the control of immunity. Nature 392: 245-252
  5. Inaba K, Witmer-Pack M, Inaba M, Hathcock KS, Sakuta H, Azuma M, Yagita H, Okumura K, Linsley PS, Ikehara S, Muramatsu S, Hodes RJ, Steinman RM. 1994. The tissue distribution of the B7-2 costimulator in mice: Abundant expression on dendritic cells in situ and during maturation in vivo. J Exp Med 180: 1849-1860
  6. Rattis FM, Peguet-Navarro J, Staquet MJ, Dezutter- Dambuyant C, Courtellemont P, Redziniac G, Schmitt D. 1996. Expression and function of B7-1 (CD80) and B7-2 (CD86) on human epidermal Langerhans cells. Eur J Immunol 26: 449-453
  7. Liu YJ, Arpin C. 1997. Germinal center development. Immunol Rev 156: 111-126
  8. Kikuchi T, Ohno N, Ohno T. 2002. Maturation of dendritic cells induced by Candida beta-D-glucan. Int Immunopharmacol 2: 1503-1508
  9. Kim GY, Lee MY, Lee HJ, Moon DO, Lee CM, Jin CY, Choi YH, Jeong YK, Chung KT, Lee JY, Choi IH, Park YM. 2005. Effect of water-soluble proteoglycan isolated from Agaricus blazei on the maturation of murine bone marrow-derived dendritic cells. Int Immunopharmacol 5: 1523-1532
  10. Lee JK, Lee MK, Yun YP, Kim YS, Kim JS, Kim YS, Kim KJ, Han SS, Lee CK. 2001. Acemannan purified from Aloe vera induces phenotypic and functional maturation of immature dendritic cells. Int Immunopharmacol 1: 1275-1284
  11. Nabeshima S, Murata M, Hamada M, Chong Y, Yamaji K, Hayashi J. 2004. Maturation of monocyte-derived dendritic cells by Hochu-ekki-to, a traditional Japanese herbal medicine. Int Immunopharmacol 4: 37-45
  12. Takei M, Tachikawa E, Hasegawa H, Lee JJ. 2004. Dendritic cells maturation promoted by M1 and M4, end products of steroidal ginseng saponins metabolized in digestive tracts, drive a potent Th1 polarization. Biochem Pharmacol 68: 441-452
  13. Kim SH, Lee SE, Oh H, Kim SR, Yee ST, Yu YB, Byun MW, Jo SK. 2002. The radioprotective effects of Bu- Zhong-Yi-Qi-Tang: A prescription of traditional Chinese medicine. Am J Chin Med 30: 127-137
  14. Jo SK, Yu YB, Oh H, Kim SR, Kim SH. 2000. The effects of Shi-Quan-Dai-Bu-Tang and its ingredients on the survival of jejunal crypt cells and hematopoietic cells in irradiated mice. J Korean Soc Food Sci Nutr 29: 93-98
  15. Kim SH, Lee SE, Oh H, Yang JA, Chung CY, Jang JS, Yu YB, Jo SK. 1999. The radioprotective effect of Kuei-Pi- Tang as a prescription of traditional Chinese medicine in mice. J Korean Soc Food Sci Nutr 28: 698-704
  16. Oh H, Park HR, Jeong IY, Kim SH, Jo SK. 2002. Protective effects of Paeonia japonica against radiation-induced damage. J Korea Asso Radiat Prot 27: 181-188
  17. Kim SH, An MR, Nah SY, Lee JH, Kim JH, Jo SK, Jang SJ, Shin DH. 2001. The effects of herbs on the radiation- induced apoptosis in intestinal crypt cells. J Korea Asso Radiat Prot 26: 27-33
  18. Kim SH, Oh H, Kim SR, Jo SK, Byun MW, Kim KS, Lee JH, Shin DH. 2001. The radioprotective effects of radices herbs. Korea J Vet Res 41: 105-111
  19. Lee SE, Oh H, Yang JA, Jo SK, Byun MW, Yee ST, Kim SH. 1999. Radioprotective effects of two traditional Chinese medicine prescriptions: Si-Wu-Tang and Si-Jun-Zi-Tang. Am J Chin Med 27: 387-396
  20. Kim SH, Oh H, Lee SE, Jo SK, Byun MW. 1998. Effect of Si-Wu-Tang and Si-Jun-Zi-Tang on the survival of jejunal crypt cells and hematopoietic cells in irradiated mice. Korean J Food Sci Technol 30: 888-894
  21. Park HR, Kim SH, Yee ST, Byun MW, Jo SK. 2005. The effects of a herb mixture (HIM-I) on the protection of the hematopoietic-immune system and self-renewal tissues against radiation damage. J Korean Soc Food Sci Nutr 34: 605-612
  22. Jo SK, Park HR, Jung UH, Oh H, Kim SH, Lee SE. 2005. Protective effect of a herbal preparation (HemoHIM) on the self-renewal tissues and immune system against $\gamma$- irradiation. J Korean Soc Food Sci Nutr 34: 805-813
  23. Son YI, Egawa S, Tatsumi T, Richard RE Jr, Kalimski P, Kanto T. 2002. A novel bulk-culture method for generating mature dendritic cells from mouse bone marrow cells. J Immunol Methods 262: 145-157

Cited by

  1. Immunological Synergistic Effects of Combined Treatment with Herbal Preparation (HemoHIM) and Red Ginseng Extracts vol.44, pp.2, 2015,
  2. Inhibitory Effects of a New Herbal Composition (HemoHIM) on UVB-Induced Suppression of Langerhans Cell's Accessory Cell Function vol.21, pp.12, 2011,