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Effects of a Herbal Composition (HemoHIM) on the Activation of Dendritic Cells

생약복합조성물(HemoHIM)의 수지상세포 활성화 효과

  • Shin, Sung-Hae (Dept. of Biology, College of Natural Science, Sunchon National University) ;
  • Kim, Do-Soon (Dept. of Biology, College of Natural Science, Sunchon National University) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University) ;
  • Jo, Sung-Kee (Radiation Food and Biotechnology Team, Advanced Radiation Technology Institute, Jeongeup Campus of Korea Atomic Energy Research Institute) ;
  • Byun, Mung-Woo (Radiation Food and Biotechnology Team, Advanced Radiation Technology Institute, Jeongeup Campus of Korea Atomic Energy Research Institute) ;
  • Yee, Sung-Tae (Dept. of Biology, College of Natural Science, Sunchon National University)
  • 신성해 (순천대학교 생물학과) ;
  • 김도순 (순천대학교 생물학과) ;
  • 김성호 (전남대학교 수의학과) ;
  • 조성기 (한국원자력연구소 방사성연구원 방사선식품생명공학팀) ;
  • 변명우 (한국원자력연구소 방사성연구원 방사선식품생명공학팀) ;
  • 이성태 (순천대학교 생물학과)
  • Published : 2006.12.29

Abstract

In our previous study, a novel herb mixture (HIM-I) of Angelica gigas radix, Cnidium officinale rhizoma, and Paeonia japonica radix was developed to protect= the intestinal and immune systems and to promote their recovery from radiation damage. A new herbal composition (HemoHIM) with the high immune modulating activity was developed from HIM-I. In the present study, we examined the effects of HemoHIM on the maturation process of murine bone marrow (BM)-derived dendritic cells (DC). BM cells were cultured in the presence of iL-4 and GM-CSF and the generated immature DC were stimulated with HemoHIM for 24 hours. HemoHIM significantly enhanced the expression of co-stimulatory molecules, CD80 and CD86, especially. The activation capacity of HemoHIM-treated DC was significantly higher than that of immature DC, as analyzed by IL-2 and $IFN-\gamma$ production and proliferation of the responding T cells in the co-culture with allogeneic T cells. The antigen-presenting capacity of HemoHIN-treated DC was also increased by the co-culture with OVA-specific T cells (HS-1), as analyzed by IL-2 and $IFN-\gamma$ production and the proliferation. These results indicate that HemoHIM causes the maturation and ;Activation of DC, which may be a part of mechanisms of immunomodulation by HemoHIM.

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  1. Immunological Synergistic Effects of Combined Treatment with Herbal Preparation (HemoHIM) and Red Ginseng Extracts vol.44, pp.2, 2015, https://doi.org/10.3746/jkfn.2015.44.2.182
  2. Inhibitory Effects of a New Herbal Composition (HemoHIM) on UVB-Induced Suppression of Langerhans Cell's Accessory Cell Function vol.21, pp.12, 2011, https://doi.org/10.5352/JLS.2011.21.12.1761