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Effects of Silk Protein Hydrolysates on Blood Glucose and Serum Lipid in db/db Diabetic Mice

실크단백질 효소 가수분해물이 2형 당뇨 마우스의 혈당 및 혈청지질에 미치는 영향

  • Shin, Mi-Jin (Dept. of Food Science and Nutrition, Dankook University) ;
  • Park, Min-Jeong (Dept. of Food Science and Nutrition, Dankook University) ;
  • Youn, Myung-Sub (Dept. of Food Science and Nutrition, Dankook University) ;
  • Lee, Young-Sook (Dept. of Food Science and Nutrition, Dankook University) ;
  • Nam, Moon-Suk (Dept. of Internal Medicine, Inha University, College of Medicine) ;
  • Park, In-Sun (Dept. of Anatomy, Inha University, College of Medicine) ;
  • Jeong, Yoon-Hwa (Dept. of Food Science and Nutrition, Dankook University)
  • 신미진 (단국대학교 식품영양학과) ;
  • 박민정 (단국대학교 식품영양학과) ;
  • 윤명섭 (단국대학교 식품영양학과) ;
  • 이영숙 (단국대학교 식품영양학과) ;
  • 남문석 (인하대학교 의과대학 내과학교실) ;
  • 박인선 (인하대학교 의과대학 해부학교실) ;
  • 정윤화 (단국대학교 식품영양학과)
  • Published : 2006.12.29

Abstract

This study was performed to investigate the effects of silk protein enzyme hydrolysates on blood glucose and serum lipid in db/db diabetic mice. Twelve week-old-male C57BL/KsJ db/db mice were divided into two groups: diabetic control group and 0.25% silk protein hydrolysates solution group, which were fed for 8 weeks. Body weight increased in the silk protein hydrolysates group compared with the diabetic control group. There were no differences in food and water intake between the diabetic control and the silk protein hydrolysates groups. The weight of liver increased in the silk protein hydrolysates group while that of kidney increased in the diabetic control group. The blood glucose level increased about 18.0% in the diabetic control group after 8 weeks while that in the silk protein hydrolysates group increased about 5.8%. Also, silk protein hydrolysates improved the glucose tolerance in C57BL/KsJ db/db mice. There was no difference in total cholesterol and non-HDL cholesterol concentration between the diabetic control and the silk protein hydrolysates group. Triglyceride concentration were lower in the silk protein hydrolysates group than in the diabetic control group (p<0.05) while HDL-cholesterol concentration were higher in the silk protein hydrolysates group than in the diabetic control group (p<0.05). This results suggest that administration of silk protein enzyme hydrolysates reduces significantly an increasing rate of 1]food glucose, decreases triglyceride, and increases HDL-cholesterol in C57BL/KsJ db/db mice.

References

  1. Nam H. 1999. Development of bioactive peptides and its market trend. Food Ind Nutr 4: 17-19
  2. Lee CH. 1992. Application and development of protein sources. Kor J Food Sci Ind 25: 93-100
  3. Rupnow J. 1994. Proteins: biochemistry and application. In Encyclopedia of Food Science and Technology. Hui YH, ed. John Willy and Son Inc, NY. Vol 3, p 2182
  4. Lu X, Akiyama D, Hirabayashi K. 1994. Production of silk powder and properties. J Seric Jpn 63: 21-27
  5. Chen K, Takanono R, Hirabayashi K. 1994. Production of soluble fibroin powder by hydrolysis with hydrochloric acid and physical properties. J Seri Sci Jpn 60: 358-362
  6. Takano R, Hirabayashi K, Chen K. 1991. Preparation of soluble silk fibroin powder by hydrochloric acid hydrolysis. Sov Phys Crystallogr 60: 358-362
  7. Chen K, Umeda Y, Hirabayashi K. 1996. Enzymatic hydrolysis of silk fibroin. J Seric Sci Jpn 65: 131-133
  8. Kim MK, Lee KH, Lim HJ, Lee SJ, Lee SH, Min KS. 1996. Preparation protocols for the functional polypeptide materials from cocoon. Korean Patent 98712
  9. Kim DK, Kim YH, Kim KB, Chin YG. 2001. The changes of molecular weight and structure in the preparation process of silk fibroin powder. J Kor Fiber Soc 38: 105-110
  10. Nahm JH, Oh YS. 1995. A study of pharmacological effect of silk fibroin. RDA J Agric Sci 37: 145-157
  11. Lee KH, Chung SH. 2000. Antidiabetic effect and mechanism of Mori rolium streptozotocin-induced diabetic mouse. Bull K H Pharm Sci 28: 87-99
  12. Cho MR, Chou RC, Chung SH, Ryu JW. 1998. Effects of silkworm powder on blood glucose and lipid levels in NIDDM (type II) patients. Korean J Nutr 31: 1139-1150
  13. Choi JH, Kim DI, Park SH, Back SJ, Kim NJ, Ryu KS. 2003. Development of anti-diabetes drink using with silkworm (Bombyx mori L.) extract. Korean J Seric 45: 96-102
  14. Bailey CJ. 1999. Insulin resistance and antidiabetic drugs. Biochem Pharmacol 58: 1511-1520 https://doi.org/10.1016/S0006-2952(99)00191-4
  15. Zhang BB, Moller DE. 2000. New approaches in the treatment of type 2 diabetes. Curr Opin Chem Biol 4: 461-467 https://doi.org/10.1016/S1367-5931(00)00103-4
  16. Parker JC, Andrews KM, Rescda DM, Massefski JR, Andrews GC, Contillo LG, Stevenson RW, Singleton DH, Suleske RT. 1998. Structure-function analysis of a series of glucagon-like peptide-1 analogs. J Peptide Res 52: 398-409
  17. Lu W, Resnick HE, Jablonski KA, Jones KL, Jain AK, Howard WJ, Robbins DC, Howard BV. 2003. Non-HDL cholesterol as a predictor of cardiovascular disease in type 2 diabetes. Diabetes Care 26: 16-23 https://doi.org/10.2337/diacare.26.1.16
  18. O'Meara NM, Devery RA, Owens D, Collins PB, Johnson AH, Tomkin GH. 1990. Cholesterol metabolism in alloxan-induced diabetic rabbits. Diabetes 39: 626-636 https://doi.org/10.2337/diabetes.39.5.626
  19. Gallaher DD, Casallany AS, Shoeman DW, Olson JM. 1993. Diabetes increase excretion of urinary malonaldehyde conjugates in rats. Lipids 28: 663-666 https://doi.org/10.1007/BF02536063
  20. 도선길, 서준교, 김충섭, 원무호, 이문한, 남중희, 오양석. 1999. Silk fibroin의 제 2형 당뇨병 모델 마우스(C57BL/ KsJ-db/db)에서 혈당강하에 관한 연구. 대한당뇨병학회 심포지움. p 81
  21. 이성희, 조병남, 현창기, 주상섭. 2002. 실크 펩타이드의 생리 기능적 특징. 항산화 효과 및 면역 기능을 중심으로. 식품과학과 산업 35: 57-61
  22. Thams P, Captio K. 1999. L-arginine stimulation of glucose-induced insulin secretion through membrane polarization and independent of nitric oxide. Eur J Endocrinol 140: 87-93 https://doi.org/10.1530/eje.0.1400087
  23. Coiro V, Volpi R, Capretti L, Speroni G, Caffarri G, Chiodera P. 1997. Involvement of nitric oxide in arginine, but not glucose, induced secretion in normal men. Clin Endocrinol (Oxf) 46: 115-119 https://doi.org/10.1046/j.1365-2265.1997.d01-1745.x
  24. Shin M, Park M, Youn M, Lee Y, Nam M, Park I, Jeong Y. 2006. Effects of silk protein hydrolysates on blood glucose in C57BL/KsJ db/db mice. J Korean Soc Food Sci Nutr 35: 1166-1171 https://doi.org/10.3746/jkfn.2006.35.9.1166
  25. Hwang E, Kang B, Kim B, Lee H. 2001. Protein quality evaluation and effect of plasma lipid contents of acid hydrolysates of cocoon in rats fed by hight cholesterol, hight triglyceride and high sucrose diet. J Korean Soc Food Sci Nutr 30: 1004-1009
  26. Kim MS, Choue RW, Chung SH, Koo SJ. 1998. Blood glucose lowering effects of mulberry leaves and silkworm extracts on mice fed with high-carbohydrate diet. Korean J Nutr 31: 117-125

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