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Effect of Larva Extract of Allomyrina dichotoma on Carbon Tetrachloride-induced Hepatotoxicity in Mice

장수풍뎅이 유충 추출물이 사염화탄소에 의한 마우스의 간 손상에 미치는 영향

  • Choi, Yong-Hwa (Dept. of Plant Resources, Sangju National University) ;
  • Lee, Ki-Yeol (Chungbuk Agricultural Research and Extension Services) ;
  • Yang, Kyung-Mi (Faculty of Herbal Food Cooking & Nutrition, Daegu Haany University) ;
  • Jeong, Yun-Mi (Dept. of Food & Nutrition, Yeungnam University) ;
  • Seo, Jung-Sook (Dept. of Food & Nutrition, Yeungnam University)
  • 최용화 (상주대학교 식물자원학과) ;
  • 이기열 (충청북도 농업기술원) ;
  • 양경미 (대구한의대학교 한방식품조리영양학부) ;
  • 정윤미 (영남대학교 식품영양학과) ;
  • 서정숙 (영남대학교 식품영양학과)
  • Published : 2006.12.29

Abstract

This study was conducted to investigate the effect of methanol extract of Allomyrina dichotoma larva (MEAL) on carbon tetrachloride $(CCl_4)-induced$ hepatotoxicity in mice. ICR mice were divided into 5 groups [Vehicle control, $CCl_4\;(10{\mu}g/g)$ alone, $CCl_4$ plus a low dose $(50{\mu}g/g)$ of MEAL, $CCl_4$ plus a high dose $(100{\mu}g/g)$ of MEAL]. Silymarin $(2{\mu}g/g)$ was used as the reference in the experiment. Administration of MEAL tended to decrease the serum alanine transaminase (ALT) activity induced by $CCl_4$ treatment in mice. Hepatic concentration of thiobarbituric acid-reactive substances (TBARS) in a high-dose group of diet decreased to the level of silymarin-treated group. Hepatic activity of glutathione S-transferase in MEAL-treated group was lower than that of $CCl_4-treated$ group. Serum concentration of bilirubin was significantly increased by $CCl_4$ treatment, but MEAL or silymarin recovered the level. These results suggest that MEAL may exert the protective effect against $CCl_4-induced$ hepatotoxicity in mice. However, more intensive studies would be needed to elucidate the protective mechanism of the beetle on hepatotoxicity of mice.

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