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Effect of Agaricus blazei β-Glucan and Egg Shell Calcium Complex on Bone Metabolism in Ovariectomized Rats

난소절제 흰쥐에서 신령버섯의 β-Glucan과 난각 Ca 복합체가 골 대사에 미치는 효과

  • Noh, Kyung-Hee (School of Food and Life Sciences, Food Science institute, and Biohealth Product Research Center, Inje University) ;
  • Jang, Ji-Hyun (School of Food and Life Sciences, Food Science institute, and Biohealth Product Research Center, Inje University) ;
  • Kim, Jin-Ju (School of Food and Life Sciences, Food Science institute, and Biohealth Product Research Center, Inje University) ;
  • Park, Cherl-Woo (HK Biotech Co., Ltd.) ;
  • Kim, Jeong-Ok (HK Biotech Co., Ltd.) ;
  • Kim, Jae-Cherl (School of Food and Life Sciences, Food Science institute, and Biohealth Product Research Center, Inje University) ;
  • Song, Young-Sun (School of Food and Life Sciences, Food Science institute, and Biohealth Product Research Center, Inje University)
  • 노경희 (인제대학교 의생명공학대학 식품생명과학부, 식품과학연구소 및 바이오헬스소재연구센터) ;
  • 장지현 (인제대학교 의생명공학대학 식품생명과학부, 식품과학연구소 및 바이오헬스소재연구센터) ;
  • 김진주 (인제대학교 의생명공학대학 식품생명과학부, 식품과학연구소 및 바이오헬스소재연구센터) ;
  • 박철우 ((주) HK 바이오텍) ;
  • 김정옥 ((주) HK 바이오텍) ;
  • 김재철 (인제대학교 의생명공학대학 식품생명과학부, 식품과학연구소 및 바이오헬스소재연구센터) ;
  • 송영선 (인제대학교 의생명공학대학 식품생명과학부, 식품과학연구소 및 바이오헬스소재연구센터)
  • Published : 2006.12.29

Abstract

This study was designed to evaluate the effect of Agaricus blazei $\beta-glucan$ and egg shell calcium complex on bone metabolism in ovariectomized (OVX) rats. Forty Sprague-Dewley female rats, 10 weeks of age $(248{\pm}1.7g)$, were divided into 4 groups and fed on the experimental diets for 6 weeks: sham operated control treated with normal diet containing 0.5% calcium (Sham-C), OVX-control treated with normal diet containing 0.5% calcium (OVX-C), $OVX-\beta-glucan$ group treated with $\beta-glucan$ diet containing 0.5% calcium (OVX-G), and $OVX-\beta-glucan$ egg shell calcium complex treated with $OVX-\beta-glucan$ egg shell calcium complex containing 0.5% calcium (OVX-GE). Bone weight of femur was higher in the OVX-GE group than in the other OVX groups. Bone mineral density of femur was significantly different (p<0.05) among the experimental groups and showed the highest level in the OVX-GE group. Calcium absorption rate and retention were higher in the $\beta-glucan$ supplement groups than in the other groups (p<0.05). Alkaline phosphatase activities and osteocalcin levels of serum showed lower in the $\beta-glucan$ supplement groups than in the OVX-C group. Deoxypyridinoline crosslink values of urine, indicator of bone absorption, showed the lowest in the OVX-GE group. The $\beta-glucan$ supplemented groups had a lower bone resorption ratio than in the OVX-C group. We concluded that bioavailability of calcium is higher in $\beta-glucan$ supplement groups compared to those in OVX rats. From the above results, these findings suggest the possibility of using $\beta-glucan$ egg shell calcium complex as a functional food material related to bone metabolism, even though there is no significant difference between the groups of $\beta-glucan$ and $\beta-glucan-egg$ shell calcium complex supplementation.

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