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Identification and Physiological Characteristics of Microorganism Isolated from Spoiled Sweetened Adzuki Ann

부패된 팥앙금으로부터 분리된 미생물의 동정과 그 균의 생리적 특성

  • 이태규 (우석대학교 대학원 생명공학과) ;
  • 노민환 (우석대학교 대학원 생명공학과)
  • Published : 2006.12.29

Abstract

In order to determine the causes of sweetened adzuki ann spoilage, the characteristics of microorganism isolated from spoiled adzuki ann were investigated. The isolated microorganism was gram-positive, roil-shaped and shore-forming bacteria; its surface was smooth and glazed. From the results of the assimilation test of 46 different biochemicals by the Vitec 2 Compact test and comparison of the cellular wall composition of fatty acid by the data bank of Midi sherlock system, the microorganism was identified as Bacillus subtilis, D-value of the B. subtilis spore was 4.85 min at $115^{\circ}C$, 0.69 min at $121^{\circ}C$ and 0.48 min at $125^{\circ}C$; Z-value was 9.71. The Bacillus subtilis growth was not observed below water activity of 0.92 at $45^{\circ}C$. However, bacteria growth increased gradually as water activity increased above 0.93.

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