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Effects of Pb and CO2 on Soil Microbial Community Associated with Pinus densiflora-Lab

소나무(Pinus densiflora) 생육토양의 미생물 군집에 미치는 납과 CO2의 영향

  • Hong, Sun-Hwa (Department of Environmental Science and Engineering, Ewha Woman's University) ;
  • Kim, Sung-Hyun (Department of Life Science, Ewha Woman's University) ;
  • Kang, Ho-Jeong (Department of Environmental Science and Engineering, Ewha Woman's University) ;
  • Ryu, Hee-Wook (Department of Chemical and Environmental Engineering, Soongsil University) ;
  • Lee, Sang-Don (Department of Environmental Science and Engineering, Ewha Woman's University) ;
  • Lee, In-Sook (Department of Life Science, Ewha Woman's University) ;
  • Cho, Kyung-Suk (Department of Environmental Science and Engineering, Ewha Woman's University)
  • 홍선화 (이화여자대학교 환경공학과) ;
  • 김성현 (이화여자대학교 생명과학과) ;
  • 강호정 (이화여자대학교 환경공학과) ;
  • 류희욱 (숭실대학교 환경화학공학과) ;
  • 이상돈 (이화여자대학교 환경공학과) ;
  • 이인숙 (이화여자대학교 생명과학과) ;
  • 조경숙 (이화여자대학교 환경공학과)
  • Published : 2006.12.31

Abstract

Effects of Pb and $CO_2$ on soil microbial community associated with Pinus densiflora were investigated using community level physiological profiles (CLPP) and 16S rDNA PCR-denaturing gradient gel electrophoresis (DGGE) methods. Two-years pine trees were planted in Pb-contaminated soils and uncontaminated soils, and cultivated for 3 months in the growth chamber where $CO_2$ concentration was controlled at 380 or 760 ppmv. The structure of microbial community was analyzed in 6 kinds of soil samples (CA-0M : $CO_2$ 380 ppmv + Pb 0 mg/kg + initial, CB-0M : $CO_2$ 380 ppmv + Pb 500 mg/kg + initial, CA-3M : $CO_2$ 380 ppmv + Pb 0 mg/kg + after 3 months, CB-3M : $CO_2$ 380 ppmv + Pb 500 mglkg + after 3 months, EA-3M : $CO_2$ 760 ppmv + Pb 0 mg/kg + after 3 months, EB-3M : $CO_2$ 760 ppmv + Pb 500 mg/kg + after 3 months). After 3 months, the substrate utilization in the uncontaminated soil samples (CA-3M vs EA-3M) was not significantly influenced by $CO_2$ concentrations. However, the substrate utilization in the Pb-contaminated soil samples (CB-3M vs EB-3M) was enhanced by the elevated $CO_2$ concentrations. The results of principal component analysis based on substrate utilization activities showed that the structure of microbial community structure in each soil sample was grouped by Pb-contamination. The similarities of DGGE fingerprints were 56.3 % between the uncontaminated soil samples (CA-3M vs EA-3M), and 71.4% between the Pb-contaminated soil samples (CB-3M vs. EB-3M). The similarities between the soil samples under $CO_2$ 380 ppmv (CA-3M vs CB-3M) and $CO_2$, 760 ppmv (EA-3M vs EB-3M) were 53.3% and 35.8%, respectively. These results suggested that the structure of microbial community associated with Pinus densiflora were sensitively specialized by Pb-contamination rather than $CO_2$ concentration.

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