The Relationship Between Stomatal Opening and Photosynthetic Activity of the Mesophyll in Commelina Communis L.

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Lee, Joon-Sang

  • 발행 : 2006.12.31

초록

To investigate the influence of the mesophyll cells on stomatal opening in response to white light, the segments of isolated epidermis were transferred on partly exposed mesophyll cells of a leaf and stomatal apertures were measured. Transferring the isolated epidermis on partly exposed mesophyll cells of a leaf caused a marked increase on stomatal apertures while stomata in isolated epidermis incubated in MES buffer hardly opened. Mesophyll infiltration with photosynthetic inhibitors (DCMU, DCCD, $NaN_3$) was performed to elucidate the correlation between stomatal apertures and the degree of photosynthetic activity. It was found that transferring the isolated epidermis on partly exposed mesophyll cells of a leaf caused an increase of stomatal apertures depending on the degree of photosynthetic activities. In $NaN_3$ infiltrated leaf discs, transferring the fresh isolated epidermis on partly exposed mesophyll cells of a leaf showed no significant effect, but a slight increase on stomatal apertures. Isolated epidermis alone did not respond to the light properly, but if it was closely contacted with mesophyil cells, the stomata regained the ability of the light response. Therefore, it could be suggested that stomatai apertures were related with the degree of photosynthetic activity in the mesophyll cells.

키워드

Commelina communis;Guard cells;Mesophyll cells;Stomatal opening

참고문헌

  1. Frechilla, S., L. D. Talbott and E. Zeiger, 2004, The blue light-specific response of Vicia faba stomata acclimates to growth environment, Plant Cell Physiol., 45, 1709-1714 https://doi.org/10.1093/pcp/pch197
  2. Zeiger, E., L. D. Talbott, S. FrechiIla, A. Srivastava and J. Zhu, 2002, The guard cell chloroplast: a perspective for the twenty-first century, New Phyto., 153, 415-424 https://doi.org/10.1046/j.0028-646X.2001.NPH328.doc.x
  3. Willmer, C. M. and M. Fricker, 1996, Stomata, Chapman & Hall, London, 289pp
  4. Tsionsky, M., Z. G. Cardon, A. J Bard and R. B. Jackson, 1997, photosynthetic electron transport in single guard cells as measured by scanning electrochemical microscopy, Plant Physiol., 113, 895-901 https://doi.org/10.1104/pp.113.3.895
  5. Sack, F. D., 1987, The development and structure of stomata, In E. Zeiger, G. D. Farquhar, I R. Cowan(e.d.), Stomatal function, Stanford University Press, Stanford, CA, pp.59-90
  6. Shimazaki, K. I and E. Zeiger, 1985, Cyclic and noncyclic photophosphorylation in isolated guard cell chloroplasts from Vicia faba L, Plant Physiol., 78, 211-214 https://doi.org/10.1104/pp.78.2.211
  7. Zeiger, E., 1990, Light perception in guard cells, Plant Cell Environ., 13, 739-747 https://doi.org/10.1111/j.1365-3040.1990.tb01088.x
  8. Lu, P, H. W. Jr. Outlaw, B. G. Smith and G. A. Freed, 1997, A new mechanism for the regulation of stomatal aperture size in intact leaves: accumulation of mesophyll-drived sucrose in the guard -cell wall of Vicia faba L, Plant Physiol., 114, 109-114 https://doi.org/10.1104/pp.114.1.109
  9. Asai, N., N. Nakazima, M. Tamaoki, H. Kamada and N. Kondo, 2000, Role of malate synthesis mediated by phosphoenolpyruvate carboxylase in guard cells in the regulation of stomatal movements, Plant Cell Physiol., 41, 10-15 https://doi.org/10.1093/pcp/41.1.10
  10. Lawson, T., K. Oxborough, I. L. James, J. I. L. Morison and N. R. Baker, 2002, Responses of photosynthetic electron transport in stomatal guard cells and mesophyll cells in intact leaves to light, $CO_{2}$, and humidity, Plant Physiol., 128, 52-62 https://doi.org/10.1104/pp.010317
  11. Lawson, T., K. Oxborough, J. I. L. Morison and N. R. Baker, 2003, The responses of guard and mesophyll cell photosynthesis to $CO_{2}$, $O_{2}$, light, and water stress in a range of species are similar, J. Exp. Bot., 54, 1743-1752 https://doi.org/10.1093/jxb/erg186
  12. Caemmerer, S. V., T. Lawson, K. Oxborough, N. R. Baker, T. J. Andrews and C. A. Raines, 2004, Stomatal conductance does not correlate with photosynthetic capacity in transgenic tobacco with reduced amounts of Rubisco, J. Exp. Bot., 55, 1157-1166 https://doi.org/10.1093/jxb/erh128
  13. Shirke, P. A. and U. V. Pathre, 2004, Influence of leaf-to air vapour pressure deficit (VPD) on the biochemistry and physiology of photosynthesis in Prosopis juliflora, J. Exp. Bot., 55, 2111-2120 https://doi.org/10.1093/jxb/erh229
  14. Weyers, J. D. B. and A. J. Travis, 1981, Selection and preparation of leaf epidermis for experiments on stomatal physiology, J. Exp. Bot., 32, 837-850 https://doi.org/10.1093/jxb/32.4.837
  15. Kitajima, J. and W. L. Butler, 1975, Quenching of chlorophyll fluorescence and primary photochemistry in the chloroplasts by dibromothymoquinone, Biochem. Biophy. Acta, 376, 105-115 https://doi.org/10.1016/0005-2728(75)90209-1
  16. Demmig, B. and O. Bjorkman, 1987, Comparison of the effect of excessive light of chlorophyll fluorescence (77k) and photon yield of $O_{2}$ evolution in leaves of higher plants, Planta, 171, 1171-1184
  17. Poffenroth, M., D. B. Green and G. Tallman, 1992, Sugar concentrations in guard cells of Vicia faba illuminated with red or blue light, Analysis by high performance liquid chromatography, Plant Physiol., 98, 1460-1471 https://doi.org/10.1104/pp.98.4.1460
  18. Talbott, G. and E. Zeiger, 1993, Sugar and organic acid accumulation in guard cells of Vicia faba in response to red and blue light, Plant Physiol., 102, 1163-1169 https://doi.org/10.1104/pp.102.4.1163
  19. Tyree, M. T. and P. Yianoulis, 1980, The site of water evaporation from sub-stomatal cavities, liquid path resistance and hydroactive stomatal closure, Annals Bot., 46, 175-193 https://doi.org/10.1093/oxfordjournals.aob.a085906
  20. Boyer, J. S., 1985, Water transport, Ann. Rev. Plant Physiol., 36, 473-516 https://doi.org/10.1146/annurev.pp.36.060185.002353
  21. Ntsika, G. and S. Delrot, 1986, Changes in apoplastic and intracellular leaf sugars induced by the blocking of export in Vicia faba, Physiol. Plant., 68, 145-153 https://doi.org/10.1111/j.1399-3054.1986.tb06610.x
  22. Van-Bel, A. J. E., 1993, Strategious of phloem loading, Ann. Rev. Plant Physiol. Plant Mole. Bio., 44, 253-281 https://doi.org/10.1146/annurev.pp.44.060193.001345
  23. Lohaus, G., H. Winter, B. Riens and H. W. Heldt, 1995, Further studies of the phloem loading process in leaves of barley and spinach, The comparison of metabolite concentrations in the apoplastic compartment with those in the cytosolic compartment and in the sieve tubes, Bot. Acta, 108, 270-275 https://doi.org/10.1111/j.1438-8677.1995.tb00860.x
  24. Reckman, U., R. Scheibe and K. Raschke, 1990, Rubisco activity in guard cells of Pisum sativum compared with the solute requirement for stomatal opening, Plant Physiol., 92, 246-253 https://doi.org/10.1104/pp.92.1.246
  25. Outlaw, W. H. and D. B. Fisher, 1975, Compartmentation in Vicia faba leaves, I. Kinetics of $^{14}C$-sucrose redistribution in the tissues following pulse labeling, Plant Physiol., 55, 699-703 https://doi.org/10.1104/pp.55.4.699
  26. Outlaw, W. H., D. B. Fisher and A. L. Christy, 1975, Compartmentation in Vicia faba leaves, II. Kinetics of $^{14}C$-sucrose redistribution among individual tissues following pulse labeling, Plant Physiol., 55, 704-711 https://doi.org/10.1104/pp.55.4.704
  27. Dittrich, P. and K. Raschke, 1977, Uptake and metabolism of carbohydrates by epidermal tissue, Planta, 134, 83-90 https://doi.org/10.1007/BF00390099
  28. Willmer, C. M., N. Thorpe, J. C. Rutter and F. L. Milthorpe, 1978, Stomatal metabolism: carbon dioxide fixation in attached and detached epidermis of Commelina, Aust. J. Plant Physiol., 5, 767-778
  29. Lee, J. S. and D. J. F. Bowling, 1992, Effect of the mesophyll on stomatal opening in Commelina communis, J. Exp. Bot., 43, 951-957 https://doi.org/10.1093/jxb/43.7.951
  30. Trejo, C. L., W. J. Davies and L. M. P. Ruiz, 1993, Sensitivity of stomata to ABA: an effect of the mesophyll, Plant Physiol., 102, 497-502 https://doi.org/10.1104/pp.102.2.497
  31. Nelson, S. D. and J. M. Mayo, 1975, The occurrence of functional nonchlorophyllous guard cells in Paphiopedilum spp, Canadian J. Bot., 53, 1-7 https://doi.org/10.1139/b75-001
  32. Wong, S. C., I. R. Cowan and G. D. Farquhar, 1979, Stomatal conductance correlates with photosynthetic capacity, Nature, 282, 424-426 https://doi.org/10.1038/282424a0
  33. Lee, J. S. and D. J. F. Bowling, 1995, Influence of the mesophyll on stomatal opening, Aust. J. Plant Physiol., 22, 357-383 https://doi.org/10.1071/PP9950357
  34. Thorpe, N. and F. L. Milthorpe, 1984, Transport of metabolites between the mesophyll and epidermis of Commelina cyanea R.Br, Aust. J. Plant Physiol., 11, 59-68
  35. Talbott, L. D., I. J. Shimayevich, Y. Chung, J. W. Hammad and E. Zeiger, 2003, Blue light and phytochrome-mediated stomatal opening the npq1 and phot1 phot2 mutants of arabidopsis, Plant Physiol., 133, 1522-1529 https://doi.org/10.1104/pp.103.029587
  36. Maier-Maerker, U., 1983, The role of peristomatal transpiration in the mechanism of stomatal movement, Plant Cell Environ., 6, 369-380 https://doi.org/10.1111/j.1365-3040.1983.tb01269.x
  37. Hetherington, A. M. and F. I. Woodword, 2003, The role of stomata in sensing and driving environmental change, Nature, 424, 901-908 https://doi.org/10.1038/nature01843
  38. Outlaw, W. H., 1989, Critical examination of the quantitative evidence for and against $CO_{2}$ fixation by guard cells, Physiol. Plant., 77, 275-281 https://doi.org/10.1111/j.1399-3054.1989.tb04981.x
  39. Kinoshita, T., M. Doi, N. Suetsugu, T. Kagawa, M. Wada and K. shimazaki, 2001, Phot 1 and Phot 2 mediate blue light regulation of stomatal opening, Nature, 414, 656-660 https://doi.org/10.1038/414656a
  40. Outlaw, W. H., 1996, Stomata: Biophysical and biochemical aspects, In N. R. Baker(ed.), Photosynthesis and the Environment, Kluwer Academic Publishers, Dordrecht, The Netherlands, pp.241-259
  41. Pickard, W. F., 1982, Distribution of evaporation in the sub-stomatal chamber, the possibility of transpiration-linked pore narrowing, and the pathway of water near the site of evaporation, Annals Bot., 49, 545-548 https://doi.org/10.1093/oxfordjournals.aob.a086280
  42. Assmann, S. M., 1999, The cellular basis of guard cell sensing of rising $CO_{2}$, Plant Cell Environ., 22, 629-637 https://doi.org/10.1046/j.1365-3040.1999.00408.x
  43. Tallman, G. and E. Zeiger, 1988, Light quality and osmoregulation in Vicia gaurd cells, Evidence for involvement of three metabolic pathways, Plant Physiol., 88, 887-895 https://doi.org/10.1104/pp.88.3.887
  44. Spanswick, R. M., 1981, Electrogenic ion pumps, Ann. Rev. Plant Physiol., 32, 267-289 https://doi.org/10.1146/annurev.pp.32.060181.001411
  45. Gotow, K., S. Taylor and E. Zeiger, 1988, photosynthetic carbon fixation in guard cell protoplasts of Vicia faba, Evidence from radiolabel experiments, Plant Physiol., 86, 700-705 https://doi.org/10.1104/pp.86.3.700
  46. Yianoulis, P. and M. T. Tyree, 1984, A model to investigate the effect of evaporative cooling on the patteren of evaporation in sub-stomatal cavities, Annals Bot., 53, 189-206 https://doi.org/10.1093/oxfordjournals.aob.a086680