An Electrical Signal Detection System Using Nanoparticle for a Microbiochip

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  • Published : 2006.01.01


A system for the electrical bio signal detection for a microchip is proposed. Gold nanoparticles were selected for the system for their bio-compatibility and potential for higher sensitivity with large surface areas. For the estimation of the conductivity of gold nanoparticles, microchips with interdigitated microelectrodes of 3,5,7 and $9\;{\mu}m$ spacing were fabricated. In addition, a simulation program was developed to estimate the electrical resistance of the fabricated microchip. The results of conduction simulation for the nanoparticles show good agreements with experimental data, which validate the proposed system.


Microbiochip;Electrical Detection;Nanoparticle;Conductivity;Microelectrode;MEMS


  1. Nicolini, C., 1995, 'From Neural Chip and Engineering Biomolecules to Bioelectronic Devices : and Overview,' Biosensors & Bioelectronics, Vol. 1, No. 1/2, pp. 105-127
  2. Park.J.G, 2000, 'Biochip Technology and Application,' Proceeding of KIEE, Vol. 49, No. 2, pp. 17-23
  3. Albers, J., Grunwald, T., Nebling, E., Piechotta, G., Hintsche, R., 2003, 'Electrical Biochip Technology - a Tool for Microarrays and Continuos Monitoring,' Analytical and Bioanalyical Chemistry, Vol. 377, No. 3, pp. 521-527
  4. Vo-Dinh, T., Cullum, B., 2000, 'Biosensors and Biochips : Advance in Biological and Medical Diagnostics,' Fresenius Journal of Analytical Chemistry, Vol. 366, pp. 540-551
  5. Harris, T., 2000, 'Genetics, Genomics and Drug Discovery,' Medicinal Research Reviews, Vol. 20, pp. 203-211<203::AID-MED4>3.0.CO;2-G
  6. Kerman, K., Kobayashi. M., Tamiya, E., 2004, 'Recent Trends in Electrochemical DNA Biosensor Technology,' Measurement science & technology,' Vol.15, pp. R1-R11
  7. Yoshio O., Yuka M., Kuniharu I., Masayuki M., Akia M., and Keisuke M., 1992, 'Hybridization of Nuclec Acids Immobilized on a Quartz Crystal Microbalance,' Journal of the American Chemical Society, Vol. 114, pp. 8299-8300
  8. Schwarz, M. A., Hauser, P. C., 2001, 'Recent Development in Detection Methods for Microfabricated Analytical Devices,' Lab on a chip, Vol. 1,pp. 1-6
  9. Wang, J., 2003, 'Nanoparticle-Based Electrochemical DNA Detection,' Analytica Chimica Acta, Vol. 500. pp. 247-257
  10. Piunno, P., Krull, U., Hudson, R., Dahma, M., Cohen, H., 1995, 'Fiber-Optic DNA Sensor for Fluorometric Nucleic Acid Determination' Analytical Chemistry, Vol. 67, pp. 2635-2643
  11. Park.S.J, Taton, T.A., Mirkin, C. A., 2002, 'Array-Based Electrical Detection with Nanoparticle probes,' SIENCE, Vol. 295, No. 5559, pp. 1506-1508
  12. Burnmeister, J., Bazilyanska, V., Grothe, K., Koehelr, B., Dorn, I., Warner, B.D., Diesel, E., 2004, 'Single Nucleotide Polymorphism Analysis by Chip-Based Hybridization and Direct Current Electrical Detection of Gold-Labeled DNA,' Analytical Bioanalyticl Chemisty, Vol. 379, No. 3, pp. 391-398
  13. Haguet, V., Martin, D., .Marcon, L., Heim, T., Stievenard, D., Oliver, C., El-Mahdi, O., Melnyk, O., 2004, 'Combined Nanogap Nanoparticles Nanosensor for Electrical Detection of Biomolecular Interactions Between Polypeptides' Applied Physics Letters, Vol. 84, No. 7, pp. 12113-1215
  14. Moller, R., Csaki, A., Kohler, J. M., Fritzsche, W., 2001, 'Electrical Classification of the Concentration of Bioconjugated Metal Colloids After Surface Adsorption and Silver Enhancement,' Langmuir, Vol. 14, pp. 5426-5430
  15. Hyatt, M.A., 1989, 'Colloidal Gold : Principles, Methods and Applications,' Academic Press, pp. 1-32
  16. Park, J.J., 2005, 'A Study on an Electrical Biosignal Detection System for the Microbiochip' KSPE, Vol. 22. No. 4