DOI QR코드

DOI QR Code

PDMS/Glass Based DNA Microbiochip for Restriction Enzyme Reaction and Electrophoresis Detection

DNA의 제한효소 반응 및 전기영동 검출용 PDMS/유리 마이크로바이오칩

  • 최준영 (한양대학교 대학원 정밀기계공학과) ;
  • 안유민 (한양대학교 기계공학과) ;
  • 황승용 (한양대학교 분자생명과학부)
  • Published : 2006.01.01

Abstract

This paper reports low-cost PDMS/glass based DNA microbiochip for the restriction enzyme reaction and its products detection using the capillary electrophoresis. The microbiochip ($25mm{\times}75mm$) has the heater integrated reactor ($5{\mu}{\ell}$) for DNA restriction enzyme reaction at $37^{\circ}C$ and the microchannel ($80\;{\mu}m{\times}100\;{\mu}m{\times}58mm$) for the capillary electrophoresis detection. It is experimentally confirmed that the digestion of the plasmid ($pGEM^{(R)}-4Z$) by the enzyme (Hind III and Sca I) is performed for less than 10 min and its electrophoresis detection is able to sequentially on the fabricated microbiochip.

Keywords

DNA;Restriction Enzyme;Electrophoresis;Microbiochip

References

  1. Stephen, C., Jacobson, J, and Ramsey, M., 1996, 'Integrated Microdevice for DNA Restroction Fragment Analysis,' Anal. Chem., Vol. 68, pp.720-723 https://doi.org/10.1021/ac951230c
  2. Burns, M. A., Johnson, B. N., Brahmasandra, S. N., Hansdique, K., Webster, J. R., Krishnan, M., Sammarco, T. S., Man, P. M., Jones, D., Heldsinger, D., Mastrangelo, and Burke, D. T., 1998, 'An Integrated Nanoliter DNA Analysis Device,' Science, Vol. 282, pp. 484-487 https://doi.org/10.1126/science.282.5388.484
  3. Zhan, Z., Dafu, C., Zhongyao, Y. and Li, W., 2000, 'Biochip for PCR Amplification in Silicon,' 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine & Biology, Lyon, France, pp. 25-28 https://doi.org/10.1109/MMB.2000.893733
  4. Schabmueller, C. G. J., Evans, A. G. R., Brunnschweiler, A., Ensell, G, Leslie, D. L. and Lee, M. A., 2000, 'Design, Fabrication and Packaging of Closed Chamber PCR-Chips for DNA Amplification,' Proceedings of SPIE-The International Society for Optical Engineering, Vol. 4019, pp. 362-369 https://doi.org/10.1117/12.382271
  5. Hong, J. W., Fujii, T., Seki, M., Yamamoto, T. and Endo, I., 2001, 'Integration of Gene Amplification and Capillary Gel Electrophoresis on a Polydimethylsiloxane-Glass Hybrid Microchip,' Eletrophoresis, Vol. 22, pp. 328-333 https://doi.org/10.1002/1522-2683(200101)22:2<328::AID-ELPS328>3.0.CO;2-C
  6. SchneegaB, I., Brautigam, R., and Kohler, J. M., 2001, 'Miniaturized Flow-Through PCR with Different Template Types in a Silicon Chip Thermocycler,' Lab on Chip, Vol. 1, pp. 42-49 https://doi.org/10.1039/b103846j
  7. Fujii, T., 2002, 'PDMS-Based Microfluidic Devices for Biomedical Applications,' Microelectron. Eng., Vol. 61-62 pp. 907-914 https://doi.org/10.1016/S0167-9317(02)00494-X
  8. Yoon, D. S., Lee, Y.-S., Lee, Y. Cho, H. J., Sung, S. W. Oh, K. W., Cha, J. and Lim, G., 2002, 'Precise Temperature Control and Rapid Thermal Cycling in a Micromachined DNA Polymerase Chain Reaction Chip,' J. Micromech. Microeng., Vol. 12, pp. 813-823 https://doi.org/10.1088/0960-1317/12/6/312
  9. Shin, Y. S., Cho, K., Lim, S. H., Chung, S., Park, S.-J., Chung, C., Han, D.-C. and Chang, J. K., 2003, 'PDMS-Based Micro PCR Chip with Parylene Coating,' J. Micromech. Microeng. Vol. 13, pp. 768-774 https://doi.org/10.1088/0960-1317/13/5/332
  10. Liu, R. H., Yang, J., Lenigk, R., Bonanno, J., and Grodzinski, P., 2004, 'Self-Contained, Fully Integrated Biochip for Sample Preparation, Polymerase Chain Reaction Amplification, and DNA Microarray Detection,' Anal. Chem., Vol. 76, pp. 1824-1831 https://doi.org/10.1021/ac0353029
  11. Jin, S.-H., Cho, Y.-J. and Ahn, Y., 2004, 'Study on PDMS/Glass Microthermostat Fabrication and Evaluation for Restriction Enzyme Reaction,' Trans. of the KSME (A), Vol. 28, No. 10, pp. 1598-1602 https://doi.org/10.3795/KSME-A.2004.28.10.1598
  12. Cho, C.-H., Cho, W., Ahn, Y., Huh, Y.-S., Yeom, H.-J., and Hwang, S.-Y., 'PDMS/Glass Microbiochip for Biochemical Reaction Application,' Proceedings of the KSME 2005 Spring Annual Meeting, pp. 1740-1744
  13. Campbell, N. A, Reece, J. B., and Simon, E. J., 2004, Essential Biology, 2nd ed., Benjamin Cummings, San Francisco, pp. 220-223