Effects of Compost Amendment on Petroleum Hydrocarbon Removal and ATP Concentration in Bioremediation of Diesel Contaminated Soil

디젤오염토앙의 생물학적 복원에 있어서 유기질비료의 첨가가 석유계 탄화수소의 분해 및 ATP 변화에 미치는 영향

  • Lee, Joo-Heon (Division of Architecture, Civil and Environmental Engineering, Taegu University) ;
  • Jun, Kwan-Soo (School of Civil and Environmental Engineering, Yeungnam University)
  • 이주헌 (대구대학교 건설환경공학부) ;
  • 전관수 (영남대학교 건설환경공학부)
  • Published : 2006.07.31


The effects of compost amendment on the removal of petroleum hydrocarbons and the activities of microorganisms in soil ecosystem have been studied in bioremediation of diesel contaminated soil. The relation between biological activities and removal of petroleun hydrocarbon was determined by ATP(Adenisine Triphosphate), n-alkanes and TPH concentration analysis. After 80 days of bioremediation, the removal of TPH in soil amended with compost increased more than 10% compared with control soil which was tilled in the same condition without compost addition. The biodegradations of n-alkanes having 12 to 20 moles of carbon were distinctive. As the soil was contaminated with more diesel, the ATP has decreased rapidly. When the TPH amounted to 80,000 mg diesel/kg, the ATP decreased to 4 ng/g from initial concentration of 65 ng/g. While the ATP in the compost amended soil increased to 112 ng/g after tilling for 6 days, the ATP in the control increased to merely 36 ng/g after tilling for 14 days. Also while the control soil showed a lag time in ATP increase, the compost amended soil did not show that but showed a rapid ATP increase within a short time. The patterns of changes in ATP concentration were similar to those in daily removals of TPH with time difference of about 7 days.


  1. Fan, C. Y. and Tafuri, A. N., 'Engineering application of bio-oxidation processes for treating petroleum-contaminated soil,' in Remediation of hazardous waste contaminated soil, Wise, D. L. and Trantolo, D., J.(Eds.), Marcel Dekker Inc., pp. 373(1994)
  2. Eweis, J. B., Ergas, S. J., Chang D. P. Y, and Schroeder, E. D., Bioremediation principles, WCB/McGraw-Hill, 8(1998)
  3. 이민효, '토양 지하수 오염문제의 현황과 향후 전망,' 환경보전, 9/10, 8-16(2000)
  4. 권기석, 윤병대, 'Bioremediation 기술개발과 미생물의 역할,' 생물산업, 9(3), 3017-3024(1996)
  5. Wang, X. and Bartha, R., 'Effects of bioremediation on toxicity, mutagenesis, and microbiota in hydrocarbon polluted soils,' in Remediation of hazardous waste con- taminated soils, Wise, D. L. and Trantolo, D., J.(Eds.), Marcel Dekker Inc., pp. 175(1994)
  6. Atlas, R. M., 'Microbial degradation of petroleum hydrocarbons: An environmental perspective,' Microbiol. Rev., 45(1), 180-209(1981)
  7. 김무훈, 강순기, 정우성, 박덕신, '유류오염지역의 미생물분포 및 활성도에 관한 연구,' 대한환경공학회지, 21(11), 2041-2047(1999)
  8. 박현철, 최선열, 이태호, 박태주, '혐기성슬러지 첨가에 따른 디젤오염토양내 TPH의 변화,' 대한환경공학회 춘계학술대회(2004)
  9. Hur, J. M. and Park, J. A., 'Effect of sewage sludge mix ratio on the biodegradation of diesel-oil in a contaminated soil composting,' Korean J. Chem. Eng., 20(2), 307-314(2003) https://doi.org/10.1007/BF02697246
  10. Lee, J. J., Kim, S. M., Lee, M. J., and Chung, S. Y., 'A study on the conditions for aerobic treatment of oil-contaminated soil by crude oil degrading bacteria using respirometer,' 한국폐기물학회 춘계학술회의논문집, pp. 271-276(2002)
  11. 한국지하수토양환경학회, 토양환경공학, 향문사, 33(2003)
  12. Autry, A. R. and Ellis, G. M., 'Bioremediation of petroleum fuel contaminated soils,' Federal Environmental Restoration Conference Proceedings, pp. 93-100(1993)
  13. Peramaki, M. P. and Blomker, K. R., 'Practical design considerations for composting contaminated soil,' In Situ and On-Situ Bioremediation, Battelle Press, pp. 103-112(1997)
  14. Prince, R. C., 'Petroleum spill bioremediation in marine environments,' Crit. Rev. in Microbiol., 19(4), 217-242 (1993) https://doi.org/10.3109/10408419309113530
  15. Douglas, G. S., Bence, A. E., Prince, R. C., McMillen, S. J., and Butler, E. L., 'Environmental stability of selected petroleum hydrocarbon source and weathering ratios,' Environ. Sci. Technol., 30(7), 2332-2339(1996) https://doi.org/10.1021/es950751e
  16. King, D. H. and Perry, J. J., 'The origin of fatty acids in the hydrocarbon-utilizing microorganism,' Can. J. of Microbiol., 21, 85-89(1975) https://doi.org/10.1139/m75-012
  17. Jurtshuk, P. and Cardini, G. E., 'The mechanism of hydrocarbon oxidation by a Corynebacterium species,' Crit. Rev. in Microbiol., 1, 239-289(1971) https://doi.org/10.3109/10408417109104483
  18. Markovetz, A. J., 'Subterminal oxidation of aliphatic hydrocarbons by microorganisms,' Crit. Rev. in Microbiol., 1, 225-237(1971) https://doi.org/10.3109/10408417109104482
  19. Pimik, M. P., 'Microbial oxidation of methyl branched alkanes,' Crit. Rev. in Microbiol., 5, 413-422(1977) https://doi.org/10.3109/10408417709102812
  20. Atlas, R. M., 'Petroleum microbiology,' Encyclopedia of Microbiology, 3, Academic Press, 363-369(1992)
  21. Baker, K. H. and Herson, D. S., 'Microbiology and biodegradation,' Bioremediation, Baker, K. H. and Herson, D. S.(Eds.), McGraw-Hill, pp. 9-60(1994)
  22. Schaeffer, T. L., Cantwell, S. G., Brown, J. L., Watt, D. S., and Fall, R. R., 'Microbial growth on hydrocarbons: terminal branching inhibits biodegradation,' Appl. Environ. Microbiol., 38, 742-746(1979)
  23. Beam, H. W. and Perry, J. J., 'Co-metabolism as a factor in microbial degradation of cycloparaffinic hydrocarbons,' Archives of Microbiology, 91, 87-90(1973) https://doi.org/10.1007/BF00409542
  24. Cantwell, S. G., Lau, E. P., Watt, D. S., and Fall, R. R., 'Biodegradation of a cyclic iso-prenoids by Pseudomonas species,' J. of Bacteriol., 135, 324-333(1978)
  25. Westlake, D. W. S., Jobson, A. M., and Cook, F. D., 'In Situ degradation of oil in a soil of the boreal region of the Northwest Territories,' Can. J. of Microbiol., 24, 254-260(1978) https://doi.org/10.1139/m78-044
  26. Roubal, G. and Atlas, R. M., 'Hydrocarbon biodegradation in Cook inlet,' Developments in Industrial Microbiology, 20, 498-502(1979)