Successful EDTA-Tris treatment of Pseudomonas aeruginosa infection of urinary bladder secondary to urolithiasis in a dog

  • Lee, Sang-Gwan (Joeun Animal Hospital) ;
  • Hoh, Woo-Pil (Joeun Animal Hospital) ;
  • Eom, Ki-Dong (College of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Keun-Woo (College of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Oh, Tae-Ho (College of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University)
  • Accepted : 2006.01.27
  • Published : 2006.03.29


About 8 year-old castrated male Yorkshire terrier was presented for evaluation of dysuria, stranguria, hemtauria, and pollakiuria. On history taking, dysuria first was observed three months ago and these signs were waxed and waned. Physical examination revealed mild left perineal swelling. On routine laboratory examination, no significant findings were identified. Positive contrast urogram identified peritoneal herniation of urinary bladder. Urinalysis showed proteinuria and hematuria. Urine sediment revealed epithelial cells, white blood cells and rod-shaped bacteria. Pseudomonas aeroginosa was isolated from urine obtained through cystocentesis, and had resistance against fourteen antibiotics. Cystitis caused by P. aeruginosa concurrent with cystolithiasis and perineal hernia was diagnosed. Cystotomy, herniorrhaphy and EDTA-Tris solution lavage of bladder were performed. The patient was recovered to normal condition 2 days after treatment. Two weeks later, bacterial culture of urine was negative and any abnormality in ultrasonogram and urinalysis was not observed except calcium oxalate dihydrate crystals.


  1. Ashworth CD, Nelson DR. Antimicrobial potentiation of irrigation solutions containing tris-(hydroxymethyl) aminomethane-EDTA. J Am Vet Med Assoc 1990, 197, 1513-1514
  2. Bartges JW. Urinary tract infection. In: Textbook of Veterinary Internal Medicine. 6th ed. pp. 1800-1808, Saunders, Philadelphia, 2005
  3. Blue JL, Wooley RE, Eagon RG. Treatment of experimentally induced Pseudomonas aeruginosa otitis externa in the dog by lavage with EDTA-tromethaminelysozyme. Am J Vet Res 1974, 35, 1221-1223
  4. Brown MR, Richards RM. Effect of ethylenediaminetetraacetate on the resistance of Pseudomonas aeruginosa to antibacterial agents. Nature 1965, 207, 1391-1393
  5. Brown SI, Akeya S, Weller CA. Prevention of the ulcers of the alkali-burned cornea. Preliminary studies with collagenase inhibitors. Arch Ophthalmol 1969, 82, 95-97
  6. Eagon RG, Carson KJ. Lysis of cell walls and intact cells of Psuedomonas aeruginosa by ethylenediaminetetraacetic acid and by lysozyme. Can J Microbiol 1965, 11, 139-201
  7. Farca AM, Nebbia P, Re G. Potentiation of the in vitro activity os some antimicrobial agents against selected Gram-negative bacteria by DETA-tromethamine. Vet Res Comm 1993, 17, 77-84
  8. Farca AM, Piromalli G, Maffei F, Re G. Potentiating effect of EDTA-Tris on the activity of antibiotics against resistant bacterial associated with otitis, dermatitis and cystitis. J Small Anim Pract 1997, 38, 243-245
  9. Gilleland HE, Jr., Stinnett JD, Roth IL, Eagon RG. Freeze-Etch study of Pseudomonas aeruginosa: localization within the cell wall of an ethylenediaminetetraacetic acid component. J Bacteriol 1973, 113, 417-432
  10. Goldschmidt MC, Kuhn CR, Perry K, Johnson DE. EDTA and lysozyme lavage in the treatment of pseudomonas and coliform bladder infections. J Urol 1972, 107, 969-972
  11. Goldschmidt MC, Wyss O. The role of tris in EDTA toxicity and lysozyme lysis. J Gen Microbiol 1967, 47, 421-431
  12. Gray GW, Wilkinson SG. The action of ethylenediaminetetraacetic acid on Pseudomonas aeruginosa. J Appl Bacteriol 1965, 28, 153-164
  13. Haslam DF, Best GK, Durham NN. Quantitation of the action of ethylenediamine-tetraacetic acid and tris (hydroxymethyl) aminomthane on a gram-negative bacterium by vancomycin adsorption. J Bacteriol 1970, 103, 523-524
  14. Hedlund CS. Surgery of the perineum, rectum, and anus. In: Small Animal Surgery. 2nd ed. pp. 415-447, Mosby, St. Louis, 2002
  15. Neu HC. The role of amine buffers in EDTA toxicity and their effect on osmotic shock. J Gen Microbiol 1969, 57, 215-220
  16. Russell AD. Effect of magnesium ions and ethylenediamine-tetraacetic acid on the activity of vancomycin against Escherichia coli and Staphylococcus aureus. J Appl Bacteriol 1967, 30, 395-401
  17. Senior DF. Management of difficult urinary tract infections. In: Current Veterinary Therapy XIII. Small Animal Practice. pp. 883-886, Saunders, Philadelphia. 2000
  18. Sparks TA, Kemp DT, Wooley RE, Gibbs PS. Antimicrobial effect of combinations of EDTA-Tris and amikacin or neomycin on the microorganisms associated with otitis externa in dogs. Vet Res Comm 1994, 18, 241-249
  19. Voss JG. Effects of organic cations on the gramnegative cell wall and their bactericidal activity with ethylenediamine-tetraacetic acid and surface active agents. J Gen Microbiol 1967, 48, 391-400
  20. Wilson LA. Chelatin in experimental Pseudomonas keratitis. Br J Ophthalmol 1970, 54, 587-593
  21. Wooley RE, Berman AP, Shotts EB Jr. Antibiotictromethamine-EDTA lavage for the treatment of bacterial rhinitis in a dog. J Am Vet Med Assoc 1979, 175, 817-818
  22. Wooley RE, Jones MS, Gilbert JP, Shotts EB Jr. In vitro action of combinations of antimicrobial agents with EDTA-tromethamine on Escherichia coli. Am J Vet Res 1983, 44, 1154-1158
  23. Wooley RE, Jones MS, Gilbert JP, Shotts EB Jr. In vitro effect of combinations of antimicrobial agents with EDTA-tromethamine on certain Gram positive bacteria. Am J Vet Res 1983, 44, 2167-2169
  24. Wooley RE, Schall WD, Eagon RG, Scott TA. Efficacy of EDTA-tris-lysozyme lavage in the treatment of experimentally induced Pseudomona aeruginosa cystitis in the dog. Am J Vet Res 1974, 35, 27-29
  25. Youngquist RS. Pseudomonas metritis in a mare. Vet Med Small Anim Clin 1975, 70, 340-342