Separation of racemic enantiomer carvedilol and its metabolites using capillary electrophoresis

Capillary Electrophoresis에 의한 carvedilol 및 그 대사체의 거울상이성질체 분리

  • 조천호 (경기대학교 이과대학 화학과) ;
  • 구소현 (경기대학교 이과대학 화학과) ;
  • 명승운 (경기대학교 이과대학 화학과)
  • Received : 2006.06.23
  • Accepted : 2006.08.04
  • Published : 2006.08.28

Abstract

A capillary electrophoresis method for the separation of carvedilol and its metabolites enantiomers using cyclodextrins as the chiral selectors was developed. The effect of several types of cyclodextrins, concentration and capillary temperature for enantiomer resolution were investigated. Best results were obtained by 15 mM carboxymethyl-${\beta}$-cyclodextrin in the run buffer. Also the effect of capillary temperature on efficiency was assessed. The optimized method was applied for separation of chiral carvedilol and its three metabolites.

Keywords

carvedilol;metabolite;enantiomer;capillary electrophoresis;separation

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