The study on the measurement of formaldehyde in saliva and urine by GC-MS

가스크로마토그래프-질량분석기에 의한 타액 및 뇨 중 포름알데하이드 분석법 연구

  • Shin, Ho-Sang (Department of Environmental Education, Drug Abuse Research Center, Kongju National University) ;
  • Ahn, Hye-Sil (Department of Environmental Science, Kongju National University)
  • 신호상 (공주대학교, 환경교육과, 약물남용연구소) ;
  • 안혜실 (공주대학교, 환경과학과 대학원)
  • Received : 2005.12.08
  • Accepted : 2006.02.20
  • Published : 2006.04.27

Abstract

A gas chromatography-mass spectrometric method was developed for the determination of formaldehyde in urine and saliva. In a 20 mL glass tube, 0.2 mL of urine or saliva was taken. Further, 1.8 mL of 0.1 M HCl, 0.1 mL of 2,000 mg/L 2,4-dinitrophenyl hydrazine and $20{\mu}l$ of 500 mg/L acetone-$d_6$ as internal standard were added in the tube and sealed tightly with cap. The solution was shaken for 20 min at room temperature and extracted using 4 mL of toluene. The extract was concentrated and redissolved with $100{\mu}l$ of acetonitrile, and then measured by gas chromatography-mass spectrometer (selected ion monitoring). The detection limit was 2.0 ng/mL and 0.5 ng/mL in saliva and urine, respectively. The calibration curves showed good linearity with r = 0.997 and 0.998 for saliva and urine, respectively. The method was used to analyze formaldehyde in rat urine after oral exposure. The developed method may be use ful to the monitoring for formaldehyde exposure in human.

Keywords

Gas chromatography-mass spectrometric method;formaldehyde;saliva;urine

Acknowledgement

Supported by : 환경기술진흥원

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