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Characterization of Physiological Properties in Vibrio fluvialis by the Deletion of Oligopeptide Permease (oppA) Gene

Vibrio fluvialis oligopeptide permease (oppA) 유전자 deletion에 의한 생리적 특성

  • Ahn Sun Hee (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Lee Eun Mi (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Kim Dong Gyun (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Hong Gyoung Eun (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Park Eun Mi (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Kong In Soo (Department of Biotechnology and Bioengineering, Pukyong National University)
  • 안선희 (부경대학교 생물공학과) ;
  • 이은미 (부경대학교 생물공학과) ;
  • 김동균 (부경대학교 생물공학과) ;
  • 홍경은 (부경대학교 생물공학과) ;
  • 박은미 (부경대학교 생물공학과) ;
  • 공인수 (부경대학교 생물공학과)
  • Published : 2006.02.01

Abstract

Oligopeptide is known to be an essential nitrogen nutrient for bacterial growth. Oligopeptide can be transported into cytoplasm by a specific transport system, Opp system. Opp system is composed of five proteins, which are transcribed by an operon. These are responsible for oligopeptide binding protein (OppA), permease (OppB and OppC) and energy generation system (OppD and OppF), respectively. Previously, we isolated the opp operon from Vibrio fluvialis and constructed the oppA mutant by allelic exchange method. In this study, we investigated the growth pattern and biofilm production under the different growth condition. When the cells were cultivated using brain heart infusion(BHI) medium, the wild type was faster than the mutant in growth during the exponential phase. However, it showed that the growth pattern of two strains in M9 medium is very similar. The growth of wild type showed better than that of the mutant grown at pH 8. At pH 7, there was no an obvious difference in growth. After 5 mM $H_2O_2$ was treated to the cells $(OD_{600}=1.2)$, the cell survival was examined. The oppA mutation did not affect in survivability. In the presence of $10{\mu}g/ml$ polymyxin B, the biofilm production of the oppA mutant was higher than that of the wild type.

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