Evaluation of DPPH Radical Scavenging Activity of Jeju Seaweeds Using High Throughput Screening (HTS) Technique

High Throughput Screening (HTS) 기법을 통한 제주 자생 해조류의 DPPH 라디칼 소거활성 평가

  • Kim, Kil-Nam (Faculty of Applied Marine Science, Cheju National University) ;
  • Heo, Soo-Jin (Faculty of Applied Marine Science, Cheju National University) ;
  • Cha, Seon-Heui (Faculty of Applied Marine Science, Cheju National University) ;
  • Jeon, You-Jin (Faculty of Applied Marine Science, Cheju National University)
  • 김길남 (제주대학교 해양생산과학부) ;
  • 허수진 (제주대학교 해양생산과학부) ;
  • 차선희 (제주대학교 해양생산과학부) ;
  • 전유진 (제주대학교 해양생산과학부)
  • Published : 2006.09.30

Abstract

As a rapid and quick bioactive compound evaluation technique, in this study we utilized a automatic system of High Throughput Screening (HTS) to investigate DPPH radical scavenging efficacy of seaweeds, collected from Jeju Island in Korea. In this study, 6 species of green seaweeds, 18 species of brown seaweeds and 22 species of red seaweeds extracted with methanol at $20^{\circ}C$ and $70^{\circ}C$ were subjected to HTS. Of the green seaweeds tested, Enteromorpha compressa (20G6) of the green seaweeds showed DPPH radical scavenging activity of over 60%. On the other hand, the other green seaweeds showed poor DPPH radical scavenging activities (lees than 40%) at 1 mg/ml. Sargassum siliquastrum (20B17, 70B17), Dictyota dichotoma (70B1), Sargassum coreanum (70B16) and Ecklonia cava (70B26) among the brown seaweeds showed significantly high DPPH radical scavenging activity with 96%, 97%, 92%, 92%, 87%. Polysiphonia japonica (20R24), Schizymenia dubyi (20R17), Gelidium amansii (20R18) and Acrosorium flabellatum (20R23) among the red seaweeds showed remarkable DPPH radical scavenging activity of over 90%.

Acknowledgement

Supported by : 해양수산부