DOI QR코드

DOI QR Code

In Vivo Measurements of Blood Flow in a Chicken Embryo Using Micro PIV Technique

유정란 배아 혈액유동의 in vivo 계측

  • 여창섭 (포항공과대학교 대학원 기계공학과) ;
  • 한규연 (포항공과대학교 생명과학과) ;
  • 이상준 (포항공과대학교 기계공학과)
  • Published : 2006.04.01

Abstract

To analyze in-vivo blood flow characteristics in a chicken embryo, in-vivo experiment was carried out using micro-PIV technique. Because endothelial cells in blood vessels are subject to shear stress of blood flow, it is important to get velocity field information of the placental blood flow. Instantaneous velocity fields of an extraembryonic blood vessel using a high-speed camera and intravital microscope. The flow images of RBCs were obtained with a spatial resolution of $20\times20{\mu}m$ in the whole blood vessels. The mean velocity field data confirm that the blood flow does show non-Newtonian fluid characteristic. The blood in a branched vessel merged smoothly without any flow separation into the main blood vessel with the presence of a slight bump. This in-vivo micro-PIV measurement technique can be used as a powerful tool in various blood flow researches.

References

  1. Malek, A. M., Alper, S. L. and Izumo, S., 1999, 'Hemodynamic Shear Stress and its Role .in Atherosclerosis,' Journal of American Medical Association, Vol. 282, pp. 2035-2042 https://doi.org/10.1001/jama.282.21.2035
  2. Hove, J. R., Koster, R. W., Forouhar, A. S., Acevedo-Bolton, G, Fraser. S. E. and Gharib, M., 2003, 'Intracardiac Fluid Forces are an Essential Epigenetic Factor for Embryonic Cardiogenesis,' Nature, Vol. 421, pp. 172-177 https://doi.org/10.1038/nature01282
  3. Sugii, Y., Nishio, S. and Okamoto, K., 2002, 'Measurement of a Velocity Field in Microvessels Using a High Resolution PIV Technique,' Annals of The New York Academy of Sciences, Vol. 972, pp. 331-336 https://doi.org/10.1111/j.1749-6632.2002.tb04592.x
  4. Vennemann, P., Kiger, K. T., Lindken, R., Groenendijk, B. W., Stekelenburg-de Vos, S., ten Hagen, T. M., Ursem, N. C., Poelmann, R., Westerweel, J. and Hierck, B., 2005, 'In vivo Micro Particle Image Velocimetry Measurements of Blood-plasma in the Embryonic Avian Heart,' Journal of Biomechanics, in press https://doi.org/10.1016/j.jbiomech.2005.03.015
  5. Hogers, B., DeRuiter, M. C., Gittenberger-de Groot, A. C. and Poelmann, R. E., 1999, 'Extraembryonic Venous Obstructions Lead to Cardiovascular Malformations and can be Embryolethal,' Cardiovascular Research, Vol. 41, pp. 87-99 https://doi.org/10.1016/S0008-6363(98)00218-1
  6. Santiago, J. G., Wereley, S. T., Meinhart, C. D., Beebe, D. J. and Adrian, R. J., 1998, 'A Particle Image Velocimetry System for Microfluidics,' Experimental in Fluids, Vol. 25, pp. 316-319 https://doi.org/10.1007/s003480050235
  7. Meinhart, C. D., Wereley, S. T. and Santiago, J. G., 1999, 'PIV Measurement of a Microchannel Flow,' Experimental in Fluids, Vol. 27, pp. 414-419 https://doi.org/10.1007/s003480050366
  8. Koutsiaris, A. G., Mathioulakis, D. S. and Tsangaris, S., 1999, 'Microscope PIV for Velocity-field Measurement of Particle Suspensions Flowing Inside Glass Capillaries,' Measurement Science and Technology, Vol. 10, pp. 1037-1046 https://doi.org/10.1088/0957-0233/10/11/311