Comparison of Antibacterial and Antioxidant Activities of Mushroom Mycelium Culture Extracts Cultivated in the Citrus Extracts

감귤농축액 첨가배지에서 배양한 버섯균사체 추출물의 항균활성 및 항산화활성 비교

  • Kim Man-Chul (Faculty of marine science, Cheju National University) ;
  • Kim Min-Joo (Faculty of marine science, Cheju National University) ;
  • Kim Taeg (Dae-Woo Environment Co.) ;
  • Park Guen-Tae (The Research-Industry University Liaison, Pusan National University) ;
  • Son Hong-Joo (Department of Biotechnology, Miryang National University) ;
  • Kim Gi-Young (Faculty of marine science, Cheju National University) ;
  • Choi Woo-Bong (Department of Biotechnology & Bioengineering, Dongeui University) ;
  • Oh Duck-Chul (Depariment of Life Science, Cheju National University) ;
  • Heo Moon-Soo (Faculty of marine science, Cheju National University)
  • 김만철 (제주대학교 해양과학부) ;
  • 김민주 (제주대학교 해양과학부) ;
  • 김택 ((주) 대우환경) ;
  • 박근태 (부산대학교 산학협력단) ;
  • 손홍주 (밀양대학교 생명공학과) ;
  • 김기영 (제주대학교 해양과학부) ;
  • 최우봉 (동의대학교 생명공학과/바이오물질제어학과) ;
  • 오덕철 (제주대학교 생명과학과) ;
  • 허문수 (제주대학교 해양과학부)
  • Published : 2006.02.01


This study was carried out to investigate the antimicrobial and antioxidative effects of mycelium cultural extract from mushroom. Mushroom mycelium was grown in a defined synthetic liquid medium and citrus extracts, and the culture extracts were examined for antioxidant and antibacterial activity. Myceliums of Phellinus linteus, Cordyceps militaris, Coriolus versicolor, Sparassic crispa, Agaricus blazei, lnonotus obliquus, Lentinus edodes, Hericium erinacium, Gonoderma lucidium in 10% citrus extract supplemented medium and synthesis medium were incubated in a shaking incubator (120 rpm, $24{\sim}30^{\circ}C$ ) for $7{\sim}15$ days. The antimicrobial activity of the culture fluid of mushroom mycelium grown in submerged liquid culture was tested against 12 microorganisms which were fish pathogens and common bacterial species. The culture extracts showed high activity against Vibrio sp. and had poor effect on Streptocouus sp., S. parauberis, S. iniae. The culture extracts obtained from the synthetic medium showed $30{\sim}93%$ of the 1,1-diphenyl-2-picrylhydrazyl radical scavenger activity, the culture extracts obtained from the citrus extracts medium exhibited antioxidant activity up to 55%.


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