DOI QR코드

DOI QR Code

Anticarcinogenic Effects of Extracts from Gloiopeltis tenax

참가사리 분획물의 암 예방효과

  • Jung, Young-Hwa (Dept. of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University) ;
  • Jung, Bok-Mi (Dept. of Food Science and Nutrition, Yosu National University) ;
  • Shin, Mi-Ok (Dept. of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University) ;
  • Bae, Song-Ja (Dept. of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University)
  • 정영화 (신라대학교 식품영양학과. 마린-바이오 산업화지원센터) ;
  • 정복미 (여수대학교 식품영양학과) ;
  • 신미옥 (신라대학교 식품영양학과. 마린-바이오 산업화지원센터) ;
  • 배송자 (신라대학교 식품영양학과. 마린-바이오 산업화지원센터)
  • Published : 2006.04.01

Abstract

In this study, we investigated anticarcinogenic effects of extracts from Gloiopeltis tenax (GT). GT was extracted with methanol (GTM), which was then further fractionated into four fractions by using solvent fractionation method, affording methanol (GTMM), hexane (GTMH), butanol (GTMB) and aqueous (GTMA) soluble fractions. We determined the cytotoxic effects of these fractions on cancer cells by MTT assay. Among various fractions of GT, the GTMM showed the strongest cytotoxic effect at concentration of $150{\mu}g/mL$, displaying 95.97% on HepG2 cell lines and 93.64% on HT-29 cell lines, respectively. And, the anti-proliferative effect of GT was accompanied by a marked in increase of levels of Bad, Bax, Bok and Bak protein and activation of caspase-3, caspase-7 and PARP protein. Also, we observed quinone reductase (QR) induced effects in all fraction layers of GT on HepG2 cells. The QR induced effects of the GTMM and GTMB on HepG2 cells at concentration of $60{\mu}g/mL$ showing inductive indexes of 2.86 and 2.04 compared to the control value of 1.0.

References

  1. Wynder EL, Gori GB. 1997. Contribution of environment to cancer medicine. J Natl Cancer Inst 58: 826-832
  2. Reddy L, Odhav B, Bhoola KD. 2003. Natural products for cancer prevention a global perspective. Parmacol Ther 99: 1-13 https://doi.org/10.1016/S0163-7258(03)00042-1
  3. Hartwell LH, Weinert TA. 1989. Checkpoint: controls that ensure the order of cell cycle events. Science 246: 629-634 https://doi.org/10.1126/science.2683079
  4. Banner SE, Pastorino U, Lippman SM, Hong WK. 1994. Second international cancer chemoprevention conference. Cancer Res 54: 854-860
  5. Chiarugi V, Magnelli L, Cinelli BG. 1994. Apoptosis and the cell cycle. Cell Mol Biol Res 40: 603-612
  6. Antonsson B, Martinou JC. 2000. The Bcl-2 protein family. Exp Cell Res 256: 50-57 https://doi.org/10.1006/excr.2000.4839
  7. Reed JC. 1998. The Bcl-2 family proteins. Oncogene 17: 3225-3236 https://doi.org/10.1038/sj.onc.1202591
  8. Ha YL, Michael WP. 1981. Naturally-occuring novel anticarconogenes: Conjugated dienoic derivatices of linoleic acid (CLA). J Korean Soc Food Nutr 20: 401-407
  9. Chihara G, Hamuro J, Maeda Y, Arai Y, Fukuoka F. 1970. Fractionation and purification of the polysaccharides with marked antitumor activity, especially lentinan, from Lentinus edodes (Berk.) Sing. Cancer Res 30: 2776-2781
  10. Alley MC, Scuiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, Boyd MR. 1988. Feasibility of drug screening with panel of human tumor cell line using a microculture tetrazolium assay. Cancer Res 48: 589-601
  11. Carmichael J, Degraff WG, Gazder AF, Minna JD, Mitchell JB. 1987. Evaluation of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47: 936-942
  12. Prochaska HJ, Santamaria AB. 1988. Direct measurement of NAD(P)H: Quinone reductase from cells cultured in mic-rotiter wells: A screening assay for anticarcinogenic en-zyme inducers. Anal Biochem 169: 328-336 https://doi.org/10.1016/0003-2697(88)90292-8
  13. Park YJ, Jeon KH, Kim SH, Bae SJ. 2004. The effect on antimicrobial and cytotoxicity of Brassica oleracea L. fractions. J Life Sci 14: 567-572 https://doi.org/10.5352/JLS.2004.14.4.567
  14. Jung BM, Lim SS, Park YJ, Bae SJ. 2005. Inhibitory effects on cell survival and quinone reductase induced activity of Aster yomena fractions on human cancer cells. J Korean Soc Food Sci Nutr 34: 8-12 https://doi.org/10.3746/jkfn.2005.34.1.008
  15. Bae SJ. 2004. Studies on the antioxidative and antimicrobial effects of Chondria crassicaulis. J Life Sci 14: 411-416 https://doi.org/10.5352/JLS.2004.14.3.411
  16. Bae SJ. 2004. Anticarcinogenic effects of Sargassum fulvellum fractions on several human cancer cell lines in vitro. J Korean Soc Food Sci Nutr 33: 480-486 https://doi.org/10.3746/jkfn.2004.33.3.480
  17. Rocha AB, Lopes RM, Schwartsmann G. 2001. Natural products in anticancer therapy. Curr Opin Pharmacol 1: 364-369 https://doi.org/10.1016/S1471-4892(01)00063-7
  18. Tounekti O, Pron G, Belehradek J, Mir LM. 1993. An apoptosismimetic drug that induces two types of cell death depending on the number of molecules internalized. Cancer Res 53: 5462-5469
  19. Lee JH, Lee E, Park J, Kim J. 2003. In vivo p53 function is DNA damage-induced apoptotic signaling in Drosophlia. FEBS Letters 550: 5-10 https://doi.org/10.1016/S0014-5793(03)00771-3
  20. Chiarugi V, Magnelli L, Cinelli BG. 1994. Apoptosis and the cell cycle. Cell Mol Biol Res 40: 603-612
  21. Antonsson B, Martinou JC. 2000. The Bcl-2 protein family. Exp Cell Res 256: 50-57 https://doi.org/10.1006/excr.2000.4839
  22. Reed JC. 1998. Bcl-2 family proteins. Oncogene 17: 3225- 3236 https://doi.org/10.1038/sj.onc.1202591
  23. Kaufmann SH, Desnoyers S, Ottaviano Y, Davidson NE, Poirier GG. 1993. Specific proteolytic cleavage of poly (ADP-ribose)polymerse: an early marker of chemotherapy-induced apoptosis. Cancer Res 53: 3976-3985
  24. Steinhusen U, Badock V, Bauer A, Behrens J, Wittman- Liebold B, Dorken B, Bommert K. 2000. Apoptosis-induced cleavage of $\beta$-catenin by caspase-3 result in proteolytic fragments with reduced transactivation potential. J Biol Chem 275: 16345-16353 https://doi.org/10.1074/jbc.M001458200
  25. Han EK, Arber N, Yamamoto H, Lim JT, Delohery T, Pamukcu R, Piazza GA, Xing WQ, Weinstein IB. 1998. Effects of sulindac sulfide and its matabolites on growth and apoptosis in human mammary epithelial and breast carcinoma cell lines. Breast Cancer Res Treat 48: 195-203 https://doi.org/10.1023/A:1005924730450
  26. Jeon KH, Shin MO, Bae SJ. 2005. A study on the effects of anticarcinogenic activity of Chondria crassicaulis. Korean J Nutr 38: 503-511
  27. Bae SJ. 2005. Anticarcinogenic and antioxidant effects of Rhodiola sachalinensis. J Korean Soc Food Sci Nutr 34: 1302-1307 https://doi.org/10.3746/jkfn.2005.34.9.1302

Cited by

  1. Mechanism of Inhibition of HepG2 Cell Proliferation by a Glycoprotein from Hizikia fusiformis vol.45, pp.6, 2012, https://doi.org/10.5657/KFAS.2012.0553
  2. Growth Inhibitory and Quinone Reductase Activity Stimulating Effects of Internal Organs of Aplysia kurodai Fractions on Cancer Cell Lines In vitro vol.20, pp.6, 2010, https://doi.org/10.5352/JLS.2010.20.6.877
  3. Antimicrobial- and Anticarcinogenic Activities of Amphitrite albicostatu Fractions vol.20, pp.10, 2010, https://doi.org/10.5352/JLS.2010.20.10.1505
  4. Effects of light and temperature on the attachment and development of Gloiopeltis tenax and Gloiopeltis furcata tetraspores vol.23, pp.6, 2011, https://doi.org/10.1007/s10811-010-9638-z
  5. Effect of Growth Inhibition and Quinone Reductase Activity Stimulation of Makgeoly Fractions in Various Cancer Cells vol.37, pp.3, 2008, https://doi.org/10.3746/jkfn.2008.37.3.288