Protein Composition of Domestic and Glyphosate-Tolerant Soybean

국내산 및 제초제 내성 콩의 단백질 조성 분석

  • Wei, Chun-Hua (College of Pharmacy, Chungnam National University) ;
  • Sok, Dai-Eun (College of Pharmacy, Chungnam National University) ;
  • Yang, Yun-Hyoung (Dept. of Food and Nutrition, Chungnam National University) ;
  • Oh, Sang-Hee (Dept. of Food and Nutrition, Chungnam National University) ;
  • Kim, Hyoung-Chin (Bio-Evaluation Center, korea Research Institute of Bioscience, and Biotechnology) ;
  • Yoon, Won-Kee (Bio-Evaluation Center, korea Research Institute of Bioscience, and Biotechnology) ;
  • Kim, Hwan-Mook (Bio-Evaluation Center, korea Research Institute of Bioscience, and Biotechnology) ;
  • Kim, Mee-Ree (Dept. of Food and Nutrition, Chungnam National University)
  • Published : 2006.04.01


In order to elucidate the differences of protein profiles among soybean cultivars, the protein composition of three conventional domestic soybean cultivars and two imported ones including glyphosate-tolerant HS2906 was analyzed by total nitrogen measurement, amino acid analysis and PAGE/densitometry. There were no statistically significant differences in the levels of any amino acid, including aromatic amino acids, between glyphosale-tolerant soybean and the conventional soybean WS82. In the extraction of protein, the SDS/buffer system was more efficient than the defatting/water system. The SDS-PAGE/densitometry analysis showed that there was a similar profile of proteins among cultivars, although the amount of total protein ranged from 380.2 mg/g to 423.9 mg/g. In addition, there was no discernable difference of protein profile between glyphosate- tolerant soybean (total protein amount, 380.2 mg/g) and the conventional soybean WS82 (390.2 mg/g), although the amount of ${\beta}$-conglycinin (55 kDa) was lower in glyphosate-tolerant soybean. Meanwhile, the amount of 25 kDa protein was greater in domestic soybean cultivars than imported ones. Thus, normal PAGE/ densitometry method would be useful to analyze the difference in protein profiles of soybean proteins, and furthermore Evaluate the protein profile of proteins between GMO and conventional soybean.


  1. Ju JS. 1985. Nutrition of soybean. Korea Soybean Digest 2: 16-19
  2. Utsumi S, Gidamis AB, Mikami B, Kito M. 1993. Crystallization and preliminary X-ray crystallographic analysis of the soybrean proglycinin expressed in Esch-erichia coli. J Mol Biol 233: 177-178
  3. Utsumi S, Katsube T, Ishige T, Takaiwa F. 1997. Molecular design of soybean glycinins with enhanced food qualities and development of crops producing such glycinins. Adv Exp Med Biol 415: 1-15
  4. Cho JS. 2002. Changes in dietary culture of soybean. Korea Soybean Digest 19: 34-54
  5. Kito Y, Takahashi J, Endo M, Agishi T, Kitamura S, Matsuda H, Fujiwara T, Dohi T, Ito T, Kawashima Y. 1993. The effect of LDL-apheresis on the long-term prognosis of hypercholesterolemic patients with coronary artery bypass grafts: a multicenter study. Kyobu Geka 46: 399- 404
  6. Fukushima D. 2000. Recent progress on biotechnology of soybean proteins and soybean protein food products. Proceedings of the Third International Soybean Processing and Utilization Conference, Tsukuba, Tokyo, Japan. p 11-16
  7. Hermansson AM. 1978. Some physico-chemical aspects of the structure formation of proteins. Proceedings of the FEBS Meeting Volume Date 1977, 44 (Biochem. Aspects New Protein Food). p 99-108
  8. Zhang X, Shu XO, Gao YT, Yang G, Li Q, Li H, Jin F, Zheng W. 2003. Soy food consumption is associated with lower risk of coronary heart disease in Chinese women. J Nutr 133: 2874-2878
  9. Kim SD, Hong EH, Ryu YH. 1988. Trends of soybean demand/supply and it's utilization in Korea. Korean Soybean Digest 15: 25-38
  10. Kim YH. 2002. Current achievement and perspectives of seed quality evaluation in soybean. Korean J Crop Sci 47: 95-106
  11. AOAC. 1990. Official Methods of Analysis. 15th ed. Association of official analytical chemists, Washington DC
  12. Gornall AG, Bardawill J, David MM. 1949. Determination of serum proteins by means of the biuret reaction. J Biol Chem 177: 751-766
  13. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
  14. Kim JG, Kim SG, Lee JS. 1988. Fatty acid composition and electrophoretic patterns of protein of Korean soybeans. Korean J Food Sci Technol 20: 263-271
  15. Kown SH, Oh JH, Kim JR, Song HS, Kim BW. 1975. Diversity of protein and oil contents of the Korean native soybean seeds. Korean J Breeding 7: 40-44
  16. Yaklich RW. 2001. beta-Conglycinin and glycinin in high- protein soybean seeds. J Agric Food Chem 49: 729-735
  17. KRDA. 1996. Food Composition Table. 5th ed. National Rural Living Science Institute, Korea Rural Development Administration. p 64, 538
  18. International Life Sciences Institute. 2003.
  19.  Taylor NB, Fuchs RL, MacDonald J, Shariff AR, Padgette SR. 1999. Compositional analysis of glyphosate-tolerant soy-beans treated with glyphosate. J Agric Food Chem 47: 4469-4473
  20. Zhang GY, Hayashi Y, Matsumoto S, Matsumura Y, Mori T. 2002. Molecular species of glycinin in some soybean cultivars. Phytochemistry 60: 675-681
  21. So EH, Chae YA, Kim YH, Lee YH, Yang MH. 1999. Varietal variation of 7S and 11S proteins in soybean. Korean J Breed 31: 393-399
  22. Mujoo R, Trinh DT, Ng PKW. 2002. Evaluation of soybean varieties for soymilk and tofu production potential using laboratory-developed procedures. Food Sci Biotechnol 11: 470-476

Cited by

  1. Heavy Metal and Amino Acid Contents of Soybean by Application of Sewage and Industrial Sludge vol.42, pp.2, 2013,