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Protein Composition of Domestic and Glyphosate-Tolerant Soybean

국내산 및 제초제 내성 콩의 단백질 조성 분석

  • Wei, Chun-Hua (College of Pharmacy, Chungnam National University) ;
  • Sok, Dai-Eun (College of Pharmacy, Chungnam National University) ;
  • Yang, Yun-Hyoung (Dept. of Food and Nutrition, Chungnam National University) ;
  • Oh, Sang-Hee (Dept. of Food and Nutrition, Chungnam National University) ;
  • Kim, Hyoung-Chin (Bio-Evaluation Center, korea Research Institute of Bioscience, and Biotechnology) ;
  • Yoon, Won-Kee (Bio-Evaluation Center, korea Research Institute of Bioscience, and Biotechnology) ;
  • Kim, Hwan-Mook (Bio-Evaluation Center, korea Research Institute of Bioscience, and Biotechnology) ;
  • Kim, Mee-Ree (Dept. of Food and Nutrition, Chungnam National University)
  • Published : 2006.04.01

Abstract

In order to elucidate the differences of protein profiles among soybean cultivars, the protein composition of three conventional domestic soybean cultivars and two imported ones including glyphosate-tolerant HS2906 was analyzed by total nitrogen measurement, amino acid analysis and PAGE/densitometry. There were no statistically significant differences in the levels of any amino acid, including aromatic amino acids, between glyphosale-tolerant soybean and the conventional soybean WS82. In the extraction of protein, the SDS/buffer system was more efficient than the defatting/water system. The SDS-PAGE/densitometry analysis showed that there was a similar profile of proteins among cultivars, although the amount of total protein ranged from 380.2 mg/g to 423.9 mg/g. In addition, there was no discernable difference of protein profile between glyphosate- tolerant soybean (total protein amount, 380.2 mg/g) and the conventional soybean WS82 (390.2 mg/g), although the amount of ${\beta}$-conglycinin (55 kDa) was lower in glyphosate-tolerant soybean. Meanwhile, the amount of 25 kDa protein was greater in domestic soybean cultivars than imported ones. Thus, normal PAGE/ densitometry method would be useful to analyze the difference in protein profiles of soybean proteins, and furthermore Evaluate the protein profile of proteins between GMO and conventional soybean.

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