Single Amino Acid Changes in the 5-Enolpyruvylshikimate-3-phosphate Synthase from Agrobacterium sp. Strain CP4 and Synechocystis sp. PCC6803 Alter Enzyme Activity and Glyphosate Sensitivity

  • Kang, Kyung-Su (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Min-Kyun (School of Agricultural Biotechnology, Seoul National University)
  • Published : 2006.06.30

Abstract

References

  1. Kishore, G. M. and Shah, D. M. (1988) Amino acid biosynthesis inhibitors as herbicides. Ann. Rev. Biochem. 57, 627-663 https://doi.org/10.1146/annurev.bi.57.070188.003211
  2. Herrmann, K. M. (1995) The shikimate pathway: early steps in the biosynthesis of aromatic compounds. Plant Cell 7, 907-919 https://doi.org/10.1105/tpc.7.7.907
  3. Malik, J., Barry, G. and Kishore, G. (1989) The herbicide glyphosate. Biofactors 2, 17-25
  4. Steinrucken, H. C. and Amerhein, N. (1984) 5-Enolpyruvylshikimate- 3-phosphate synthase of Klebsiella pneumonia 2. Inhibition by glyphosate [N-(phosphonomethyl)glycine]. Eur. J. Biochem. 143, 351-357 https://doi.org/10.1111/j.1432-1033.1984.tb08379.x
  5. Boocock, M. R. and Coggins, J. R. (1983) Kinetics of 5- enolpyruvylshikimate-3-phosphate synthase inhibition by glyphosate. FEBS Lett. 154, 127-133 https://doi.org/10.1016/0014-5793(83)80888-6
  6. Schonbrunn, E., Eschenburg, S., Shuttleworth, W. A., Schloss, J. V., Amrhein, N., Evans, J. N. and Kabsch, W. (2001) Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikikate 3-phosphate synthase in atomic detail. Proc. Natl. Acad. Sci. USA 98, 1376-1380
  7. Eschenburg, S., Healy, M. L., Priestman, M. A., Lushington, G. H. and Schonbrunn, E. (2002) How the mutation glycine96 to alanine confers glyphosate insensitivity to 5- enolpyruvyl shikimate-3-phophate synthase from Escherichia coli. Planta 216, 129-135 https://doi.org/10.1007/s00425-002-0908-0
  8. Sost, D. and Amerhein, N. (1990) Substitution of Gly-96 to Ala in the 5-enolpyruvylshikimate 3-phosphate synthase of Klebsiella pneumoniae results in a greatly reduced affinity for the herbicide glyphosate. Arch. Biochem. Biophys. 282, 433-436 https://doi.org/10.1016/0003-9861(90)90140-T
  9. Padgette, S. R., Re, D. B., Grasser, C. S., Eichholtz, D. A., Frazier, R. B., Hironaka, C. M., Levine, E. B., Shah, D. M., Fraley, R. T. and Kishore, G. M. (1991) Site-directed mutagenesis of a conserved region of the 5-enolpyru-vylshikimate-3-phosphate synthase active site. J. Biol. Chem. 266, 22364-22369
  10. Ye, G. N., Hajdukiewicz, P. T., Broyles, D., Rodriguez, D., Xu, C. W., Nehra, N. and Staub, J. M. (2001) Plastidexpressed 5-enolpyruvylshikimate-3-phosphate synthase genes provide high level glyphosate tolerance in tobacco. Plant J. 25, 261-270 https://doi.org/10.1046/j.1365-313x.2001.00958.x
  11. Padgette, S. R., Re, D. B., Barry, G. F., Eichholtz, D. E., Delannay, X., Fuchs, R. L., Kishore, G. M. and Fraley, R. T. (1996) New weed control opportunities: development of soybeans with a Roundup Ready gene, In Herbicide-Resistant Crops: Agricultural, Economic, Environmental, Regulatory and Technological Aspects, Duck, S.O. (ed), pp. 53-84, CRC press, Boca Raton
  12. Miller, J. H. (1972) In Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, New York
  13. Powell, H. A., Kerby, N. W. and Rowell, P. (1991) Natural tolerance to the herbicide glyphosate. New Phytol. 119, 421-426 https://doi.org/10.1111/j.1469-8137.1991.tb00042.x
  14. Chiesa, M. D., Mayes, S. R., Maskell, D. J., Nixon, P. J. and Baber, J. (1994) An aroA homologue from Synechocystis sp. PCC6803. Gene 144, 145-146 https://doi.org/10.1016/0378-1119(94)90222-4
  15. Lee, S. M., Kang K., Chung, H., Yoo, S. H., Xu, X. M., Lee, S. B., Cheong, J. J., Daniell, H. and Kim, M. (2006) Plastid transformation in the monocotyledonous cereal crop, rice (Oryza sativa) and transmission of transgenes to their progeny. Mol. Cells 21, 401-410