Characteristics of Structure and Expression Pattern of ADSF/resistin Gene in Korean Native Cattle

  • Kang, Hye Kyeong (Department of Animal Sciences & Biotechnology, Jinju National University) ;
  • Park, Ji Ae (Department of Animal Sciences & Biotechnology, Jinju National University) ;
  • Seo, Kang Seok (National Livestock Research Institute, RDA) ;
  • Kim, Sang Hoon (Department of Biology, Kyung Hee University) ;
  • Choi, Yun Jai (School of Agricultural Biotechnology, Seoul National University) ;
  • Moon, Yang Soo (Department of Animal Sciences & Biotechnology, Jinju National University)
  • Received : 2005.07.17
  • Accepted : 2005.09.01
  • Published : 2006.03.01


Adipocyte-specific secretory factor (ADSF)/resistin, a hormone, is a small cysteine-rich protein secreted from adipose tissue and has been implicated in modulating adipogenesis in humans and rodents. The objective of this study was to clone a gene encoding ADSF/resistin and to characterize its function in Korean Native Cattle (Hanwoo). The coding sequence was 330 base pairs and it encoded a protein of 109 amino acids. An NCBI BLAST-search revealed the cloned cDNA fragment shared significant homology (82%) with the cDNA encoding the human ADSF/resistin. The nucleotide sequence homology of the Hanwoo sequence was 73% and 64% for the rat and mouse, respectively. A 654 bp ADSF/resistin gene promoter was cloned and putative binding sites of transcription factors were identified. Tissue distribution of ADSF mRNA was examined in liver, skeletal muscles (tenderloin, biceps femoris), subcutaneous fat, and perirenal fat by RT-PCR. ADSF mRNAs were detected in fat tissues but not in liver and muscles, suggesting that ADSF/resistin expression may be induced during adipogenesis. Although, the physiological function of ADSF/resistin in the cow remains to be determined, these data indicate ADSF is related to the adipocyte phenotype and may have a possibly regulatory role in adipocyte function.


Supported by : Ministry of Agriculture and Forestry


  1. Ahima, R. S. and J. S. Flier. 2000. Adipose tissue as an endocrine organ. Trends Endocrinol. Metab. Review. 11(8):327-332
  2. Engert, J. C., M. C. Vohl, S. M. Williams, P. Lepage, J. C. Loredo- Osti, J. Faith, C. Dore, Y. Renaud, N. P. Burtt, A. Villeneuve, J. N. Hirschhorn, D. Altshuler, L. C. Groop, J. P. Despres, D. Gaudet and T. J. Hudson. 2002. 5' flanking variants of resistin are associated with obesity. Diabetes. 51(5):1629-1634
  3. Ghosh, S., A. K. Singh, B. Aruna, S. Mukhopadhyay and N. Z. Ehtesham. 2003. The genomic organization of mouse resistin reveals major differences from the human resistin: functional implications. Gene. 305(1):27-34
  4. Hartman, H. B, X. Hu, K. X. Tyler, C. K. Dalai and M. A. Lazar. 2002. Mechanisms regulating adipocyte expression of resistin. J. Biol. Chem. 277:19754-19761
  5. Janke, J., S. Engeli, K. Gorzelniak, F. C. Luft and A. M. Sharma. 2002. Resistin gene expression in human adipocytes is not related to insulin resistance. Obes Res. 10(1):1-5
  6. Kim, K-H. and Y. S. Moon. 2003. Molecular cloning of adipocyte tissue-specific genes by cDNA microarray. Asian-Aust. J. Anim. 16(12):1837-1841
  7. Holcomb, I. N., R. C. Kabakoff, B. Chan, T. W. Baker, A. Gurney, W. Henzel, C. Nelson, H. B. Lowman, B. D. Wright, N. J. Skelton, G. D. Frantz, D. B. Tumas, F. V. Peale, Jr., D. L. Shelton and C. C. Hebert. 2000. Fizz1, a novel cysteine-rich secreted protein associated with pulmonary inflammation, defines a new gene family. Embo. J. 19:4046-4055
  8. Kim, J. W., J. M. Hong, Y. S. Lee, S. H. Chae, C. B. Choi, I. H. Choi and J. S. Yeo. 2004. Identification of new microsatellite DNAs in the chromosomal DNA of the Korean Cattle (Hanwoo). Asian-Aust. J. Anim. 17(10):1329-1333
  9. Kim, K. H., K. Lee, Y. S. Moon and H. S. Sul. 2001. A cysteinerich adipose tissue-specific secretory factor inhibits adipocyte differentiation. J. Biol. Chem. 276:11252-11256
  10. Kim, K. H., L. Zhao, Y. Moon, C. Kang and H. S. Sul. 2004. Dominant inhibitory adipocyte-specific secretory factor (ADSF)/resistin enhances adipogenesis and improves insulin sensitivity. Proc. Natl. Acad. Sci. USA. 101(17):6780-6785
  11. Kyte, J. and R. Doolittle. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157:105-132
  12. Maebuchi, M., M. Machidori, R. Urade, T. Ogawa and T. Moriyama. 2003. Low resistin levels in adipose tissues and serum in high-fat fed mice and genetically obese mice: development of an ELISA system for quantification of resistin. Arch Biochem. Biophys. 416(2):164-170
  13. McTernan, C. L., P. G. McTernan, A. L. Harte, P. L. Levick, A. H. Barnett and S. Kumar. 2002. Resistin, central obesity, and type 2 diabetes. The Lancet. 359:46-47
  14. Patel, L., A. C. Buckels, I. J. Kinghorn, P. R. Murdock, J. D. Holbrook, C. Plumpton, C. H. Macphee and S. A. Smith. 2003. Resistin is expressed in human macrophages and directly regulated by PPAR gamma activators. Biochem. Biophys. Res. Commun. 300(2):472-476
  15. Patel, S. D., M, W. Rajala, L. Rossetti, P. E. Scherer and L. Shapiro. 2004. Disulfide-dependent multimeric assembly of resistin family hormones. Sci. 304:1154-1158
  16. Rajala, M. W., Y. Lin, M. Ranalletta, X. M. Yang, H. Qian, R. Gingerich, N. Barzilai and P. E. Scherer. 2002. Cell typespecific expression and coregulation of murine resistin and resistin-like molecule-alpha in adipose tissue. Mol. Endocrinol. 16(8):1920-1930
  17. Saltiel, A. R. 2001. You are what you secrete. Nat Med. 7(8):887- 888
  18. Sambrook, J. and D. W. Russell. 2001. Molecular cloning-A Laboratory Manual. CSHL Press. Cold Spring Harbor, New York
  19. Savage, D. B., C. P. Sewter, E. S. Klenk, D. G. Segal, A. Vidal- Puig, R. V. Considine and S. O'Rahilly. 2001. Resistin / Fizz3 expression in relation to obesity and peroxisome proliferatoractivated receptor-gamma action in humans. Diabetes. 50(10):2199-2202
  20. Seo, J. B., M. J. Noh, E. J. Yoo, S. Y. Park, J. Park, I. K. Lee, S. D. Park and J. B. Kim. 2003. Functional characterization of the human resistin promoter with adipocyte determination- and differentiation-dependent factor 1/sterol regulatory element binding protein 1c and CCAAT enhancer binding protein-alpha. Mol. Endocrinol. 17(8):1522-1533
  21. Steppan, C. M., S. T. Bailey, S. Bhat, E. J. Brown, R. R. Banerjee, C. M. Wright, H. R. Patel, R. S. Ahima and M. A. Lazar. 2001. The hormone resistin links obesity to diabetes. Nature 409:307-312
  22. Way, J. M., C. Z. Gorgun, Q. Tong, K. T. Uysal, K. K. Brown, W. W. Harrington, W. R. Oliver Jr, T. M. Willson, S. A. Kliewer and G. S. Hotamisligil. 2001. Adipose tissue resistin expression is severely suppressed in obesity and stimulated by peroxisome proliferator-activated receptor gamma agonists. J. Biol. Chem. 276(28):25651-25653